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HiScript II Q Select RT SuperMix for qPCR(+gDNAwiper)R233

HiScript II Q Select RT SuperMix for qPCR(+gDNAwiper)R233

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应用笔记

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产品介绍

HiScript II Reverse Transcriptase is a novel reverse transcriptase obtained by in vitro molecular evolution technology, based on M-MLV (RNase H-) Reverse Transcriptase, HiScript II Reverse Transcriptase greatly improves the thermal stability and cDNA synthesis efficiency. The HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) is specially designed for two-step qRT-PCR. The 4 × gDNA wiper Mix in the product can rapidly remove the residual genomic DNA contamination and ensure more reliable subsequent quantitative results. 5 × HiScript II Select qRT SuperMix II contains Buffer, dNTP mix, HiScript II Reverse Transcriptase and RNase inhibitor. Oligo(dT)23VN, Random primers or Gene Specific Primers (GSP) can be selected as reverse transcription primers according to requirements. Oligo (dT)23VN has stronger anchoring ability to Poly A+ mRNA than Oligo(dT)18, making reverse transcription more efficient. 5 × HiScript II Select qRT SuperMix does not freeze at -20℃, and the reaction proceeds quickly after adding template RNA and primers. The RT product is compatible with dye-based and probe-based qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for high-performance gene expression analysis.

产品优势

基于高效的 HiScript ® II 逆转录酶;
不同类型的逆转录引物可灵活用于不同的实验设计;
gDNAwiper Mix 可以快速彻底地去除基因组污染

组件

R233 Components

存储

All components should be stored at -20℃ for up to 12 months.

引文

Wang, Xiangxiang, et al. "Coevolution of ribosomal RNA expansion segment 7L and assembly factor Noc2p specializes the ribosome biogenesis pathway between Saccharomyces cerevisiae and Candida albicans." Nucleic Acids Research 49.8 (2021): 4655-4667.

Du, Jianfeng, et al. "Silica nanoparticles protect rice against biotic and abiotic stresses." Journal of nanobiotechnology 20.1 (2022): 1-18.

Qian, Bin, et al. "MoErv29 promotes apoplastic effector secretion contributing to virulence of the rice blast fungus Magnaporthe oryzae." New Phytologist (2022).

Ma, Rongze, et al. "LGL1 binds to Integrin β1 and inhibits downstream signaling to promote epithelial branching in the mammary gland." Cell reports 38.7 (2022): 110375.

Zheng, Jie, et al. "A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis." Cell death & disease 12.10 (2021): 1-12.

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