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HiScript II 第一链 cDNA 合成试剂盒 R211

HiScript II 第一链 cDNA 合成试剂盒 R211

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产品介绍

HiScript II Reverse Transcriptase is a novel reverse transcriptase obtained by in vitro molecular evolution technology based on M-MLV (RNase H-) Reverse Transcriptase. Compared with HiScript Reverse Transcriptase, HiScript II further improves the thermal stability and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript II adds several point mutations that further enhances template binding affinity and cDNA synthesis efficiency, and has higher tolerance to common reverse transcription inhibitors.
This product is 1st strand cDNA synthesis kit based on HiScript II Reverse Transcriptase. It contains all the components necessary for high quality first strand cDNA synthesis, and the product is suitable for subsequent PCR, qPCR and other experiments. The 2 × RT Mix contains an optimized buffer and dNTPs. The HiScript II Enzyme Mix contains the HiScript II Reverse Transcriptase and the RNase inhibitor. Oligo (dT)23VN has stronger anchoring ability for poly A+ mRNA than Oligo (dT)18, which makes reverse transcription more efficient. Oligo (dT)23VN, Random primers or Gene Specific Primers (GSP) can be selected as reverse transcription primers according to requirements. The kit can be used to synthesize full-length cDNA (up to 20 kb) for cloning and other downstream experiments, as well as highly uniform cDNA for qPCR.

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产品优势

基于高效的HiScript ® II Reverse Transcriptase,超强的耐温性保证了RNA复杂的二级结构在高温条件下可以打开,获得更长的cDNA;
模板起始量范围广,从1 pg-5 μg总RNA,可扩增片段达15 kb以上;
Anchored Oligo(dT) 23 VN专为高特异性结合位点锚定而设计,确保第一链cDNA合成的效率和成功率;
针对不同的下游应用选择不同的引物。 合成的单链cDNA广泛用于分子克隆、杂交、PCR扩增和qPCR反应等。

组件

R211 组件

存储

-30~-15℃可保存18个月,≤0℃运输。

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