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AceQ Universal SYBR qPCR Master Mix Q511
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Product Introduction
This product is a special reagent for qPCR using the SYBR® Green I chimeric fluorescence method. It uses a chemically modified hot-start DNA polymerase AceTaq® DNA Polymerase, with the optimal buffer optimized for qPCR, which can effectively inhibit non-specific amplification. Thus, the amplification efficiency is significantly improved, and it is suitable for high-sensitivity qPCR reactions. This product is a 2× premixed reagent containing the optimal concentration of SYBR® Green I for qPCR reaction. It can obtain a good standard curve in a wide quantitative area, and accurately quantify and detect target genes with good repeatability and credibility. Degree is high. In addition, the product contains a special ROX Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments. You only need to add primers and templates when preparing the reaction system to perform amplification.
Product Advantages
Universal on all platforms: Special ROX reference dye, suitable for all qPCR instruments, no need to adjust ROX concentration on different instruments
Rigorous hot-start enzyme: AceTaq DNA Polymerase, based on chemical hot-start, provides perfect amplification specificity;
Optimized reaction body: patented buffer formula, which minimizes non-specific amplification and primer dimerization, without repeated optimization of conditions;
Excellent amplification performance: repeatable and reliable quantitative results to meet the data requirements of high-level magazines
Components
| Components | Q511-02 | Q511-03 |
| 2 × AceQ Universal SYBR qPCR Master Mix* | 4 × 1.25 ml | 5 × Q511-02 |
*It contains dNTP, Mg2+, AceTaq DNA Polymerase, SYBR Green I, Specific ROX Passive Reference Dye, etc.
Storage
Store at -30 ~ -15℃, transport at ≤0℃, and protect from light.
Citation
Chen, Suzhen, et al. "The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity." Cell Metabolism 33.3 (2021): 565-580.Impact Factor:27.287
Wang, Dezhong, et al. "FGF1ΔHBS prevents diabetic cardiomyopathy by maintaining mitochondrial homeostasis and reducing oxidative stress via AMPK/Nur77 suppression." Signal transduction and targeted therapy 6.1 (2021): 1-12.Impact Factor:18.187
Huang, Jing, et al. "Inhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury." Cell Death & Differentiation 28.4 (2021): 1174-1192.Impact Factor:15.828
Zhang, Zhi, et al. "HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis." Molecular Therapy-Nucleic Acids 24 (2021): 711-727.Impact Factor:8.886
Chen, Dawei, et al. "PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3." Cell death & disease 12.8 (2021): 1-11.Impact Factor:8.469
Application Notes Abstract
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