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This product is a special reagent for qPCR using the SYBR® Green I chimeric fluorescence method. It uses a chemically modified hot-start DNA polymerase AceTaq® DNA Polymerase, with the optimal buffer optimized for qPCR, which can effectively inhibit non-specific amplification. Thus, the amplification efficiency is significantly improved, and it is suitable for high-sensitivity qPCR reactions. This product is a 2× premixed reagent containing the optimal concentration of SYBR® Green I for qPCR reaction. It can obtain a good standard curve in a wide quantitative area, and accurately quantify and detect target genes with good repeatability and credibility. Degree is high. In addition, the product contains a special ROX Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments. You only need to add primers and templates when preparing the reaction system to perform amplification.
Universal on all platforms: Special ROX reference dye, suitable for all qPCR instruments, no need to adjust ROX concentration on different instruments
Rigorous hot-start enzyme: AceTaq DNA Polymerase, based on chemical hot-start, provides perfect amplification specificity;
Optimized reaction body: patented buffer formula, which minimizes non-specific amplification and primer dimerization, without repeated optimization of conditions;
Excellent amplification performance: repeatable and reliable quantitative results to meet the data requirements of high-level magazines
|2 × AceQ Universal SYBR qPCR Master Mix*||4 × 1.25 ml||5 × Q511-02|
*It contains dNTP, Mg2+, AceTaq DNA Polymerase, SYBR Green I, Specific ROX Passive Reference Dye, etc.
Store at -30 ~ -15℃, transport at ≤0℃, and protect from light.
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