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NGS Library Prep kits

NGS Library Prep kits

Molecular Research

Drug Discovery

NGS Library Prep kits

799.00
Article number:
NR605
VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina is specially designed for the preparation of transcriptome libraries for Illumina platform. The kit is universal and suitable for RNA library construction of RNA that have been obtained by Poly(A)-based mRNA enrichment or rRNA depletion. The kit contains two types of cDNA 2nd Strand synthesis buffer, which can be chosen for library construction for non-stranded or stranded RNA-Seq transcriptome analysis. This kit combines 2nd Strand cDNA synthesis, end-repair and dA-Tailing into one step, with no need of purification, which greatly simplifies the process of library construction and shortens the operation time. The optimized reaction system improves the library construction efficiency, is compatible with lower-input RNA, and has uniform coverage for different amounts of input-RNA. Libraries of specific sizes, which can be customized, can be obtained after size selection with magnetic beads. All the enzymes and buffer provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability.
799.00
Article number:
NR605
Number of views:
1000
Keywords:
kit
100.00
Article number:
VAHTS TM HiFi Amplification Mix is a new High-fidelity PCR amplification master mix, which is applicable to high-throughput sequencing library PCR amplification. The master mix is based on VAHTS TM HiFi DNA Polymerase, which is the new generation of DNA polymerase with high yield and fidelity engineered from Pfu DNA Polymerase. The sensitivity of this enzyme has been greatly improved, with extremely high amplification efficiency and extensive adaptability of the template. It greatly improves the amplification output and the platform period.
100.00
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Number of views:
1000
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1000
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1000
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1000
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VAHTS TM HiFi Amplification Mix is a new High-fidelity PCR amplification master mix, which is applicable to high-throughput sequencing library PCR amplification. The master mix is based on VAHTS TM HiFi DNA Polymerase, which is the new generation of DNA polymerase with high yield and fidelity engineered from Pfu DNA Polymerase. The sensitivity of this enzyme has been greatly improved, with extremely high amplification efficiency and extensive adaptability of the template. It greatly improves the amplification output and the platform period.
0.00
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Number of views:
1000
Keywords:
165.00
Article number:
N101-01
In the presence of templates and primers, T4 DNA polymerase catalyzes the synthesis of DNA along the 5´→3´ direction. This enzyme also has 3´→5´ exonuclease activity, which is stronger than DNA polymerase I. Unlike DNA polymerase I, T4 DNA polymerase does not have 5´→3´ exonuclease activity.   Connect the sticky end or blunt end quickly in 5 minutes at room temperature; T/A cloning; dsDNA cut repair   Store at -20℃
165.00
Article number:
N101-01
Number of views:
1000
Keywords:
301.00
Article number:
P507-02
301.00
Article number:
P507-02
Number of views:
1000
Keywords:
71.00
Article number:
P507-01
71.00
Article number:
P507-01
Number of views:
1000
Keywords:
0.00
Article number:
N611-02
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Article number:
N611-02
Number of views:
1000
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N611-01
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Article number:
N611-01
Number of views:
1000
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0.00
Article number:
TD601
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Article number:
TD601
Number of views:
1000
Keywords:
48.00
Article number:
N106-01
48.00
Article number:
N106-01
Number of views:
1000
Keywords:
870.00
Article number:
NA102-01
VAHTSTM AmpSeq Cancer HotSpot Panel contains 207 primer pairs that can be used to amplify hotspot mutation regions of human tumor-related genes. The products cover a total of 50 proto-oncogenes and suppressor genes. 2800 common mutation sites of oncogenes. When this product is used in conjunction with VAHTSTM AmpSeq Library Prep Kit V2 (Vazyme #NA201), 20 μl multiple amplification system needs to add 10 μl of this Panel (that is, the concentration of this Panel is 2×), which can perform highly uniform multiplex amplification on the target area. Amplifier builds library, and provides VAHTSTM AmpSeq Adapters 1-96 for Illumina (Vazyme #NA111) and VAHTSTM AmpSeq Adapters 1-96 for Ion Torrent (Vazyme #NA121) for different sequencing platforms. The premixed primer panel saves researchers the time to design and prepare primers, and is suitable for starting DNA templates as low as 1ng and a variety of complex templates, such as FFPE samples and cfDNA. The fragment size of the amplified product using this product is between 111 bp-187 bp.   Store at -30 ~ -15℃
870.00
Article number:
NA102-01
Number of views:
1000
Keywords:
vahts
the
and
of
is
vahtstm
panel
ampseq
this
for
794.00
Article number:
NDM607-01/02
VAHTS® Universal DNA Library Prep Kit for MGI is a library construction kit optimized for the MGI high-throughput sequencing platform. This kit can convert 100 pg-4 μg Input DNA into a library dedicated to MGI high-throughput sequencing platform. As a new upgraded version, VAHTS® Universal DNA Library Prep Kit for MGI greatly improves the library conversion rate and amplified library output through the overall improvement of the end repair module, the connection module and the library amplification module. It is widely applicable to a variety of Construction of PCR library of samples. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.   Efficient repair of DNA damage: effective repair of base damage, nicks, gaps and 3’ end closures High library construction efficiency: both library conversion rate and amplification output are improved Wide template compatibility: compatible with samples such as gDNA, FFPE DNA, cfDNA, Amplicons, etc. Perfect compatibility with different quality samples: DNA repair enzymes have excellent performance on FFPE DNA with different DIN values Short time-consuming library construction: the shortest single library construction only takes 90 minutes      Store at -30 ~ -15℃. Can be transported at -20 ~ 0℃
794.00
Article number:
NDM607-01/02
Number of views:
1668
Keywords:
vahts
library
the
dna
of
and
construction
kit
mgi
repair
1617.00
Article number:
NRM604-01/02
VAHTS® Universal V6 RNA-seq Library Prep Kit for MGI is a special kit for transcriptome library construction targeted for the MGI high-throughput sequencing platform. The kit contains two types of cDNA two-strand synthesis buffers, which can be selected for general transcriptome or strand-specific transcription according to needs. The kit combines two-strand synthesis, end repair, and dA-Tailing into one step. No purification steps are required in the middle, which greatly simplifies the operation process and shortens the time for library construction. The optimized reaction system improves the efficiency of library conversion, is compatible with lower initial input, and has uniform coverage for different initial amounts of RNA. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet the individual needs of different experiments. All enzymes and buffers included in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.    Store at -30 ~ -15℃.
1617.00
Article number:
NRM604-01/02
Number of views:
1473
Keywords:
vahts
the
library
for
and
kit
of
to
which
mgi
908.00
Article number:
NDM617-01/02
VAHTS® Universal Plus DNA Library Prep Kit for MGI is a DNA library construction kit developed for the MGI high-throughput sequencing platform. This kit combines DNA fragmentation, end repair and dA tailing into one step. The product does not need to be purified. It is directly connected to adapters, library enriched and sorted, and can convert 100 pg-1 μg of template DNA into MGI high-throughput The library dedicated to the sequencing platform does not require mechanical interruption of the genome, which simplifies the library construction process and shortens the operation time, and is suitable for PCR library construction. This kit is perfectly compatible with DNA from different sources and different input amounts, and only needs to adjust the fragmentation time according to the size of the target insert to obtain the required library. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.    Store at -30 ~ -15℃. Can be transported at -20 ~ 0℃
908.00
Article number:
NDM617-01/02
Number of views:
1528
Keywords:
vahts
the
and
to
library
dna
kit
is
of
mgi
1315.00
Article number:
NM108-01/02
VAHTS® DNA Adapters set8 for MGI is a special kit for BGI high-throughput sequencing platform library construction, suitable for BGI high-throughput sequencing platform multi-sample DNA library construction. The set 8 kit (NM108) contains 96 different Index connectors. All adapters provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.   Provide 24 kinds of Index tags; Strict quality control and functional verification of each batch    Store at -30 ~ -15℃.
1315.00
Article number:
NM108-01/02
Number of views:
1463
Keywords:
vahts
the
for
library
and
kit
of
sequencing
dna
strict
438.00
Article number:
NM208-01/02
VAHTS® RNA Adapters set8 for MGI is a special kit for the construction of BGI's high-throughput sequencing platform library, which is suitable for the construction of multi-sample DNA library of BGI's high-throughput sequencing platform. The set 8 kit (NM208) contains 96 different Index adapters. All adapters provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.   Strand specific library: retain the directionality of transcription; Optimal reaction system: wide compatibility of species types; Wider template compatibility range: the initial amount of RNA can be as low as 50 ng; Minimal library construction process: complete library construction and quality inspection in one day    Store at -30 ~ -15℃.
438.00
Article number:
NM208-01/02
Number of views:
1241
Keywords:
vahts
the
of
library
construction
for
and
kit
sequencing
adapters
822.00
Article number:
NDM608-01/02
VAHTS® Universal Pro DNA Library Prep Kit for MGI is a MGI high-throughput sequencing platform library construction kit containing FFPE DNA repair module, which can convert 100 pg – 1 μg Input DNA into a library dedicated to the BGI platform. This kit contains a DNA damage repair module, which can effectively repair the DNA damage caused by formalin-fixed paraffin embedding (FFPE), such as cytosine deamination, nicking and nicking, base oxidation, 3'end blocking, etc. Compatible with ordinary DNA samples and does not affect the quality of the library of normal DNA samples. Through the overall improvement of the end repair module, the connection module and the library amplification module, the library conversion rate and the output of the amplified library are greatly improved, which is widely applicable to library construction of various samples and is compatible with the targeted capture process. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.   Wide compatibility: easily cope with different species sources, different starting amounts, and different template types Simple operation: only need to adjust the fragmentation time according to the target insert size to get the required library High library yield: excellent library conversion rate and better sample quality      Store at -30 ~ -15℃.
822.00
Article number:
NDM608-01/02
Number of views:
1542
Keywords:
vahts
the
library
and
dna
to
of
module
repair
kit
93.00
Article number:
EQ121-01/02
93.00
Article number:
EQ121-01/02
Number of views:
1880
Keywords:
1124.00
Article number:
TD902-01/02
HyperactiveTM In-Situ ChIP Library Prep Kit for Illumina is a special kit developed for CUT&Tag technology. CUT&Tag technology is a new method for studying protein-DNA interaction. By fusing Protein G or Protein A with the engineered ultra-highly active Tn5 transposase, a new fusion enzyme (Hyperactive pG- Tn5/pA-Tn5 Transposase), under the guidance of the antibody, accurately target and cut the DNA sequence near the target protein. Compared with traditional ChIP-Seq, this technology has the advantages of low cell input, short experiment cycle, high signal-to-noise ratio, and good repeatability. It is especially suitable for early embryonic development, stem cells, tumors, and epigenetics. field. All reagents in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.   Simple operation: using enzyme digestion instead of ultrasonic disruption, it only takes 1 day to realize the transformation from cells to next-generation sequencing libraries High activity: DNA fragmentation activity is extremely high Low starting cell volume: compatible with as few as 50 cells High purity: high protein purity, low nucleic acid residue     BOX 1: ConA beads are stored at 2 ~ 8℃, and the remaining components are stored at room temperature. BOX 2: 5% Digitonin stored at -30 ~ -15℃, and can be stored at room temperature for 1 week; 10 × Binding Buffer -30 ~ -15 ℃ storage, 2 ~ 8 ℃ can be stored for 6 months; Store the remaining components at -30 ~ -15°C.  
1124.00
Article number:
TD902-01/02
Number of views:
2680
Keywords:
illumina
the
and
for
at
stored
high
is
of
protein
1124.00
Article number:
TD901-01/02
HyperactiveTM In-Situ ChIP Library Prep Kit for Illumina is a dedicated kit developed for CUT&Tag technology. CUT&Tag technology is a new method for studying protein-DNA interactions. By fusing Protein G or Protein A with the engineered ultra-highly active Tn5 transposase, a new fusion enzyme with dual activity (Hyperactive pG- Tn5/pA-Tn5 Transposase), under the guidance of the antibody, accurately target and cut the DNA sequence near the target protein. Compared with traditional ChIP-Seq, this technology has the advantages of low cell input, short experiment cycle, high signal-to-noise ratio, and good repeatability. It is especially suitable for early embryonic development, stem cells, tumors, and epigenetics. field. All reagents in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.   Simple operation: DNA fragmentation and adaptor ligation can be realized in one step, and the transformation from cell to next-generation sequencing library can be realized in only 9 hours Super high activity: both Protein A&Tn5 activity, extremely high DNA fragmentation activity Low initial cell volume: compatible with as few as 60 cells, even single cells High purity: high protein purity, low nucleic acid residue   BOX 1: ConA beads are stored at 2 ~ 8℃, and the remaining components are stored at room temperature. BOX 2: 5% Digitonin stored at -30 ~ -15℃, and can be stored at room temperature for 1 week; 10 × Binding Buffer -30 ~ -15 ℃ storage, 2 ~ 8 ℃ can be stored for 6 months; Store the remaining components at -30 ~ -15°C.  
1124.00
Article number:
TD901-01/02
Number of views:
3812
Keywords:
illumina
the
and
for
at
stored
high
protein
be
activity
9999.00
Article number:
S701-01/02
 Hyperactive pA-MNase for CUT&RUN is the fusion of Protein A and engineered ultra-highly active MNase to form a new fusion enzyme with both MNase exonuclease activity and Protein A activity, which is specifically suitable for protein-genome interaction research CUT&RUN technology. Compared with the traditional protein-genome interaction research method ChIP-Seq, CUT&RUN has significant advantages. This technology has a short experimental cycle, high signal-to-noise ratio, good repeatability, and low cell input. It is especially suitable for early embryo development, Research fields such as stem cells, tumors and epigenetics.   (1) Simple and fast workflow: DNA denaturation and bisulfite conversion are completed in one step, and the conversion reaction time is only 140 min (2) High yield: 10 pg-100 ng genomic DNA starting amount, recovery efficiency ≥ 80%, conversion efficiency of unmethylated cytosine ≥ 99% (3) Suitable for various downstream applications: Bisulfite-converted DNA can be used for stable PCR amplification for downstream analysis of NGS sequencing, etc. (4) Compatible with DNA templates from different sources: DNA extracted from cells or tissues of animals, plants, microorganisms; cfDNA; purified PCR products, etc.   Store at -30 ~ -15℃, and transport at -20 ~ 0℃.    
9999.00
Article number:
S701-01/02
Number of views:
1895
Keywords:
cut&run
and
for
dna
is
of
the
research
conversion
365.00
Article number:
N603-01/02
Discover-sc® Single Cell WGA Kit uses an isothermal amplification system based on Multiple Strand Displacement Amplification (MDA) and enables Phi29 DNA polymerase modified by directed evolution, which has higher amplification efficiency and amplification uniformity, and can be used in Achieve undifferentiated whole genome amplification of 1-1000 cells or small samples within 2 hours. The single-cell genome can reach more than 95% coverage after N603 amplification. The size of the amplified product is between 2-100 kb, and the average product length is greater than 20 kb. It is widely used for preimplantation chromosome detection (PGS/PGD) , Tumor cells, stem cells, metagenomics and other research fields. All enzymes and buffers in Discover-sc® Single Cell WGA Kit have undergone strict quality control and functional verification. The optimized experimental system maximizes the uniformity of amplification and ensures stability and repeatability.   Low template starting volume; High amplification sensitivity; Good product integrity; Operation success rate is high; Wide volume compatibility    Store at -30 ~ -15℃. For longer-term storage, please store below -70℃.
365.00
Article number:
N603-01/02
Number of views:
1924
Keywords:
single
and
amplification
the
is
product
of
cells
in
system
145.00
Article number:
N411-01/02/03
VAHTSTM DNA Clean Beads are based on the SPRI (Solid Phase Reverse Immobilization) principle and are suitable for DNA purification and fragment size sorting in the construction of high-throughput sequencing libraries. VAHTSTM DNA Clean Beads are compatible with various brands of DNA and RNA library building kits and the library building process reported in the literature. The use method is exactly the same as the currently widely used AMPure XP Beads. The yield and size distribution of the library are highly consistent with AMPure XP Beads. It can seamlessly replace AMPure XP Beads, effectively reducing your library building costs.   High cost performance; Short delivery time; It has exactly the same usage as AMPure XP Beads, no need to re-explore the conditions; The recovery rate, library size and AMPure XP Beads are highly consistent; It is suitable for DNA and RNA library building, and has good compatibility with all brands of library building reagents; Strict batch quality control, better price, shorter delivery time   Store at 2-8℃
145.00
Article number:
N411-01/02/03
Number of views:
8124
Keywords:
vahts
the
and
library
building
dna
beads
ampure
are
xp
174.00
Article number:
N412-01/02/03
VAHTSTM RNA Clean Beads is based on the principle of (Solid Phase Reverse Immobilization), which can effectively remove protein, salt ions and other impurities, and is suitable for RNA purification. VAHTSTM RNA Clean Beads and the widely used AGENCOURT® RNACLEAN®XP are used in exactly the same way. They can seamlessly replace AGENCOURT® RNACLEAN®XP, effectively reducing your experimental cost.   Effectively remove impurities; Use the same way as AGENCOURT® RNACLEAN®XP; Reduce experiment costs   Store at 2-8℃
174.00
Article number:
N412-01/02/03
Number of views:
3071
Keywords:
vahts
the
rnaclean
rna
agencourt
and
effectively
xp
impurities
used
99.00
Article number:
EQ111-01/02
99.00
Article number:
EQ111-01/02
Number of views:
3295
Keywords:
EqualbitTM dsDNA HS Assay Kit
EQ111
129.00
Article number:
EQ211-01/02
Equalbit® RNA HS Assay Kit EqualbitTM RNA HS (High Sensitivity) Assay Kit is a simple, sensitive and accurate RNA fluorescence quantitative detection kit. This kit contains fluorescence detection reagents, buffers and RNA standards. The kit is highly selective for RNA and is not affected by dsDNA. It has an excellent linear relationship with RNA samples in the range of 5 to 100 ng. It can be used for total RNA, rRNA, and rRNA at a concentration of 250 pg/µl to 100 ng/µl. The mRNA samples are accurately quantified and have excellent tolerance to some conventional contaminants such as salt, free nucleotides, proteins, solvents, detergents, etc. This product is easy to operate and can be carried out at room temperature. Before use, the fluorescence detection reagent is diluted with a buffer solution into a working solution, and then the RNA sample to be tested is added to the Qubit® Fluorometer for detection.   Accurate sensitivity: It can accurately quantify total RNA, rRNA, and mRNA samples with a concentration of 1 ng/µl to 1000 ng/µl; Ultra-high specificity: This product is highly selective for RNA, not affected by DNA, and is excellent for some conventional pollutants, such as salt, free nucleotides, proteins, solvents, detergents, etc. Tolerance Operational simplicity: This product is easy to operate. The fluorescence detection reagents are diluted with buffer solution into working solution, and then the RNA sample to be tested is added to the Qubit® Fluorometer for detection. This operation can be performed at room temperature.    The complete kit should be stored at 2~8℃, protected from light; after the first use, Equalbit RNA HS Reagent is recommended to be stored at room temperature and protected from light; Equalbit RNA HS Buffer should be stored at room temperature; Equalbit RNA HS Standard #1 and #2 should be stored at 2 ~ 8℃
129.00
Article number:
EQ211-01/02
Number of views:
2121
Keywords:
Equalbit RNA HS Assay Kit
EQ211-01/02
129.00
Article number:
EQ212-01/02
Equalbit® RNA BR Assay Kit EqualbitTM RNA BR (Broad-Range) Assay Kit is a simple, sensitive and accurate RNA fluorescence quantitative detection kit. This kit contains fluorescence detection reagents, buffers and RNA standards. The kit is highly selective for RNA and is not affected by dsDNA. It has an excellent linear relationship with RNA samples in the range of 20 to 1000 ng, and can be used for total RNA, rRNA, and rRNA with concentrations ranging from 1 ng/µl to 1000 ng/µl. The mRNA samples are accurately quantified and have excellent tolerance to some conventional contaminants such as salt, free nucleotides, proteins, solvents, detergents, etc. This product is easy to operate and can be carried out at room temperature. Before use, the fluorescence detection reagent is diluted with a buffer solution into a working solution, and then the RNA sample to be tested is added to the Qubit® Fluorometer for detection.   Simple and easy to operate Ready-to-use premix, no need to prepare working fluid, simplifying operation steps Ultra-high sensitivity, accurate quantification of 0.2-100 ng dsDNA Super specificity It detects dsDNA specifically and has excellent tolerance to some conventional pollutants Fast dye binding speed can reach saturation within 2 minutes Extremely high stability Strictly control the production process of standard products to ensure stability between batches   The complete kit should be stored at 2~8℃, protected from light; after the first use, Equalbit RNA BR Reagent is recommended to be stored at room temperature and protected from light; Equalbit RNA BR Buffer should be stored at room temperature; Equalbit RNA BR Standard #1 and #2 should be stored at 2 ~ 8℃
129.00
Article number:
EQ212-01/02
Number of views:
1816
Keywords:
Equalbit RNA BR Assay Kit
EQ212-01/02
799.00
Article number:
NR604-01/02
VAHTS® Universal V6 RNA-seq Library Prep Kit for Illumina is a special kit for transcriptome library construction developed for Illumina high-throughput sequencing platform. The kit contains two types of cDNA two-strand synthesis buffers, which can be selected according to needs Generic transcriptome or strand-specific transcription build library. The kit combines two-strand synthesis, end repair, and dA-Tailing into one step. No purification steps are required in the middle, which greatly simplifies the operation process and shortens the time for library construction. The optimized reaction system improves the efficiency of library conversion, is compatible with lower initial input, and has uniform coverage for different initial amounts of RNA. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet the individual needs of different experiments. All enzymes and buffers included in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.     Shorter time-consuming database construction; More efficient linker connection efficiency, higher library conversion rate; Wider template compatibility range, RNA starting amount can be as low as 10ng; Wide species applicability, can be applied to different species such as humans, animals, plants, etc.    Store at -30°C~-15°C
799.00
Article number:
NR604-01/02
Number of views:
4561
Keywords:
vahts
the
library
and
kit
of
for
to
can
as
1884.00
Article number:
NR603-01/02
VAHTSTM Total RNA-seq (H/M/R) Library Prep Kit for Illumina® is a special kit for chain-specific transcriptome library construction targeted for the Illumina high-throughput sequencing platform. This kit is suitable for the amount of initial template The total RNA of human, mouse, and rat is 0.1-1 μg. The difference from conventional transcriptome library is that this kit can combine rRNA (including cytoplasm 28S, 18S, 5S rRNA and mitochondrial 16S, 12S, 5.8S rRNA). ) Is removed from total RNA, leaving mRNA and other non-coding RNAs Used for the analysis of non-coding RNA such as LncRNA; degraded RNA samples can also be used for library construction with this kit. When the second-strand cDNA is synthesized in this kit, dUTP is added to label it, and the labeled second-strand is digested with UDG enzyme before PCR, so the sequencing information comes from the first-strand cDNA, which ensures the strand directionality of RNA Analyze the sequencing data to determine whether the transcript is from the sense or antisense DNA strand. The kit includes three independent packages of NR4, NR5 and NR6, which contain the library construction process All necessary enzymes, buffers, and all components have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent. Adapters and DNA and RNA purification magnetic beads need to be prepared separately. Adapters can be selected from VAHTSTM RNA Adapters (Vazyme#N803, Vazyme #N804 or Vazyme #N809, Vazyme #N810, Vazyme #N811, Vazyme#N812). For details on adapter selection, please refer to Common Problems and Solutions 6 (Page17); DNA purification magnetic Beads can choose VAHTSTM DNA Clean Beads (Vazyme #N411) or Agencourt® AMPure® XP reagent (Beckman #A63880,#A63881, #A63882); RNA purification magnetic beads can choose VAHTSTM RNA Clean Beads (Vazyme #N412) or Agencourt® RNAClean ® XP Beads (Beckman #A63987).    Fast: The minimalist operation process reduces the time for building a database by 2 h Compatibility: Compatible with a variety of RNA enrichment modules, with any combination General: Flexible and free choice of chain-specific or non-chain-specific libraries   All components are stored at -20℃
1884.00
Article number:
NR603-01/02
Number of views:
4715
Keywords:
vahts
the
and
rna
is
for
of
beads
library
vazyme
9999.00
Article number:
NR612-01/02
VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina v2 is a special kit for the construction of strand-specific transcriptome libraries developed for the Illumina high-throughput sequencing platform. This kit is suitable for starting template amount of 0.05-4 μg completeness A good chain-specific transcriptome library construction of total RNA of eukaryotes such as animals, plants, and fungi. Unlike conventional transcriptional library construction, the substrate is incorporated into dUTP during the synthesis of the second strand, and is enriched by PCR Use UDG enzyme for the U-labeled second-strand template before the library Digestion ensures that the final sequencing information comes from the first-strand cDNA, thereby preserving the strand directionality of the mRNA. Later data analysis can determine whether the transcript comes from the sense or antisense strand of DNA. This kit is an upgraded product of VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina. This version greatly simplifies the operation process, improves the library conversion efficiency, is compatible with a lower initial input, and uses magnetic bead sorting. A library of a specific length can be quickly obtained to meet the individual needs of different experiments. All enzymes and buffers included in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.    Box1: Store at 2 ~ 8℃; Box2: Store at -30 ~ -15℃. 
9999.00
Article number:
NR612-01/02
Number of views:
3549
Keywords:
vahts
the
of
library
is
for
and
kit
construction
this
9999.00
Article number:
NR611-01/02
VAHTSTM mRNA-seq V3 Library Prep Kit for Illumina® is a special kit for transcriptome library construction targeted for the Illumina high-throughput sequencing platform. This kit is suitable for activities with a starting template amount of 0.01-4 μg and good integrity. The total RNA of eukaryotes such as plants and fungi. The total RNA sample goes through the steps of mRNA isolation, mRNA fragmentation, double-stranded cDNA synthesis, end repair, adaptor ligation, ligation product purification, fragment size sorting, library amplification and other steps. Obtain a sequencing library suitable for the Illumina platform. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet the individual needs of different experiments. The kit includes three independent packages of NR1, NR21 and NR31 , Contains all the enzymes and buffers required for the library construction process. All components have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent. VAHTSTM mRNA-seq V3 Library Prep Kit for Illumina® is an upgraded version developed on the basis of VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina®, which simplifies the operation process, improves library conversion efficiency, and is compatible with lower initial investment The amount of RNA has uniform coverage for different initial amounts of RNA.    Provide 24 kinds of Index connectors; Strict quality control and functional verification of each batch   NR 1, 2-8°C storage; NR 21, stored at -20°C; NR 31, stored at -20°C
9999.00
Article number:
NR611-01/02
Number of views:
4398
Keywords:
vahts
the
of
library
for
and
kit
illumina
is
prep
9999.00
Article number:
NR602-01/02
VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina® is a special kit for transcriptome library construction targeted for the Illumina® high-throughput sequencing platform. This kit is suitable for constructing a starting template with an input amount of 0.1-4 μg total RNA A chain-specific transcriptome library of eukaryotes such as animals, plants, and fungi. The difference from conventional transcriptome library construction is that the total RNA of the sample is separated, fragmented, and first-strand cDNA is synthesized. During synthesis, dUTP was incorporated to label it, and at the same time, the end repair was completed at this step; followed by the steps of adding A-tailing, ligation adapter, ligation product purification, fragment size sorting, library amplification, etc., before PCR enrichment of the library The U-labeled second-strand template is digested with UDG enzyme. Therefore, the final sequencing information comes from the first-strand cDNA, which preserves the strand directionality of the mRNA. Data analysis after sequencing can determine whether the transcript comes from the sense strand or Antisense DNA strand. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet different needs. The kit includes three independent packages of NR1, NR5 and NR6, which contain all the enzymes and buffers needed for the library construction process. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the library to the greatest extent Stability and repeatability of the construction.    Includes Ribo-OffTM rRNA depletion module, which can remove ribosomal RNA from human, mouse, rat, cow, dog, horse, chicken, monkey, pig, zebrafish and other species; Chain-specific library construction; Optimized large fragment library construction scheme   NR 1, 2-8°C storage; NR 5, stored at -20°C; NR 6, stored at -20°C. 
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Article number:
NR602-01/02
Number of views:
2865
Keywords:
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1020.00
Article number:
NR801-01/02
VAHTSTM Small RNA Library Prep Kit for Illumina® is a special kit for constructing small RNA libraries targeted for the Illumina® high-throughput sequencing platform. This kit is suitable for the total RNA of animals and plants with a template starting amount of 0.1-1 μg, as well as the separation and purification of small RNA. Connect the 3'and 5'ends of small RNA with universal adapters, and then go through the steps of reverse transcription, PCR amplification, and purification by PAGE gel or magnetic beads to finally obtain a sequencing library suitable for the Illumina® platform. The kit contains all the enzymes and buffers required for library construction. All components have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.   RL5 Adaptor, stored at -70°C; Filtration Column, stored at room temperature; other components, stored at -20°C.
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Article number:
NR801-01/02
Number of views:
3347
Keywords:
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9999.00
Article number:
NR601-01/02
VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina® is a special kit for transcriptome library construction targeted for the Illumina® high-throughput sequencing platform. This kit is suitable for animals and plants with an initial template amount of 0.1-4 μg And fungi and other eukaryotic total RNA. The total RNA sample goes through the steps of mRNA isolation, fragmentation, double-stranded cDNA synthesis, end repair, A-tailing, adaptor ligation, ligation product purification, fragment size sorting, library amplification, etc. Finally, a sequencing library suitable for the Illumina® platform is obtained. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet the individual needs of different experiments. The kit includes three NR1, NR2 and NR3 The independent packaging contains all the enzymes and buffers required for the library construction process. All components have undergone strict quality control and functional verification to ensure the stability and repeatability of the library construction to the greatest extent. Adapters and DNA purification magnetic beads need to be prepared separately. Adapters can be selected from VAHTSTM RNA Adapters (Vazyme #N803, Vazyme #N804 or Vazyme #N809, Vazyme #N810, Vazyme #N811, Vazyme #N812). For details on adapter selection, please refer to Common Problems and Solutions 4 (Page16); DNA purification magnetic Beads can choose VAHTSTM DNA Clean Beads (Vazyme #N411) or Agencourt® AMPure® XP reagent (Beckman #A63880, #A63881, #A63882).    The chain directionality of the mRNA is retained, and the gene structure and gene expression information can be obtained more accurately; It has a high success rate for complex plant templates; The library construction process is the same as Illumina TruSeq Stranded mRNA Sample Prep Kit; Optimized large fragment library construction program; The sequencing data is highly consistent with Illumina reagents   NR 1, 2-8°C storage; NR 2, stored at -20°C; NR 3, stored at -20°C
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Article number:
NR601-01/02
Number of views:
3127
Keywords:
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877.00
Article number:
N409-01/02
Ribo-off® rRNA depletion kit (Plant) is a kit for removing rRNA from the total RNA of plant roots, seeds and leaves. This kit is suitable for total plant RNA with a template starting amount of 1-5 μg. The total RNA sample undergoes steps such as rRNA and probe hybridization, RNase H digestion, DNase I digestion and other steps, and finally rRNA (including 5S, 18S and 25S rRNA) Remove from total RNA, retain mRNA and other non-coding RNA, which can be used for the analysis of non-coding RNA such as LncRNA; the kit additionally provides digestion probes targeting mitochondrial rRNA and chloroplast rRNA, which can efficiently remove major plant species rRNA provides the most informative sequencing results. The kit is suitable for both intact and partially degraded RNA samples, and the resulting products are suitable for RNA libraries and other experiments.   High sensitivity; High coverage High uniformity High fidelity high production; easy to use; Low equipment requirements    Store at -30°C~-15°C
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Article number:
N409-01/02
Number of views:
1972
Keywords:
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