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NGS Library Prep kits

Molecular Research

Drug Discovery

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Number of views:
4621

HiScript II Reverse Transcriptase

HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation HiScript® Reverse Transcriptase, HiScript® II Transcriptase has further greatly improved thermal stability, with a half-life of more than 4 hours at 50°C, and can be reacted for a long time at 55°C to maintain stability. It is very suitable for complex secondary Reverse transcription of structural RNA template. In addition, HiScript® II Transcriptase adds a number of point mutations, which further enhances template affinity and progression, greatly improving the synthesis ability of full-length cDNA, and obtaining cDNA up to 20 kb. It has a higher tolerance to common reverse transcription inhibitors and is very suitable for the reverse transcription reaction of plant tissue RNA rich in polysaccharides and polyphenols.   The half-life at 50℃ exceeds 240 min, and the half-life at 55℃ can reach 60 min; CDNA up to 10 kb can be synthesized in 5 minutes, which is suitable for rapid reverse transcription reaction; Higher impurity tolerance and higher detection sensitivity, reverse transcriptase is the first choice for molecular diagnosis   Store at -20℃
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31.88
Market price
$
31.88
Number of views:
4621
Product serial number
R201-01/02
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Stock:
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HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation HiScript® Reverse Transcriptase, HiScript® II Transcriptase has further greatly improved thermal stability, with a half-life of more than 4 hours at 50°C, and can be reacted for a long time at 55°C to maintain stability. It is very suitable for complex secondary Reverse transcription of structural RNA template. In addition, HiScript® II Transcriptase adds a number of point mutations, which further enhances template affinity and progression, greatly improving the synthesis ability of full-length cDNA, and obtaining cDNA up to 20 kb. It has a higher tolerance to common reverse transcription inhibitors and is very suitable for the reverse transcription reaction of plant tissue RNA rich in polysaccharides and polyphenols.

 

The half-life at 50℃ exceeds 240 min, and the half-life at 55℃ can reach 60 min;
CDNA up to 10 kb can be synthesized in 5 minutes, which is suitable for rapid reverse transcription reaction;
Higher impurity tolerance and higher detection sensitivity, reverse transcriptase is the first choice for molecular diagnosis

 

Store at -20℃

Keyword:
R201-01/02
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