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NGS Library Prep kits

Molecular Research

Drug Discovery

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HiScript 1st Strand cDNA Synthesis Kit

HiScript® Reverse Transcriptase is a brand-new reverse transcriptase obtained through multiple mutations on the basis of M-MLV (RNase H-) Reverse Transcriptase. HiScript® Reverse Transcriptase has the highest activity at 50°C and can withstand reaction temperatures as high as 55°C. It can be used for reverse transcription of RNA templates with secondary structure. By enhancing the affinity with the template, more full-length cDNA can be obtained, and the total RNA can be detected as low as 1 pg. It is especially suitable for reverse transcription of a small number of templates and low-copy genes. In addition, the mismatch rate of HiScript® Reverse Transcriptase is also lower than that of M-MLV (RNase H-) Reverse Transcriptase, which improves the fidelity of cloning. The HiScript® First-strand cDNA Synthesis Kit is based on HiScript® Reverse Transcriptase and contains all the ingredients needed to synthesize high-quality first-strand cDNA, suitable for subsequent PCR, qPCR and PCR cloning. 2 × RT Mix contains optimized buffer system and dNTP; Enzyme Mix contains HiScript®Reverse Transcriptase and RNase inhibitor. Oligo (dT)18, Random hexamers, or gene-specific primers can be selected as reverse transcription primers according to needs.    HiScript ® Reverse Transcriptase provides reliable reverse transcription performance; A wide range of template amount, which can be successfully reverse transcribed from 10 pg-5 μg total RNA; Efficient synthesis ability: can synthesize up to 10 kb cDNA; Different primers can be selected for different downstream applications. The synthesized one-strand cDNA can be widely used in molecular cloning, hybridization, PCR amplification, and qPCR reactions.    Store at -20℃
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Product serial number
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HiScript® Reverse Transcriptase is a brand-new reverse transcriptase obtained through multiple mutations on the basis of M-MLV (RNase H-) Reverse Transcriptase. HiScript®
Reverse Transcriptase has the highest activity at 50°C and can withstand reaction temperatures as high as 55°C. It can be used for reverse transcription of RNA templates with secondary structure. By enhancing the affinity with the template, more full-length cDNA can be obtained, and the total RNA can be detected as low as 1 pg. It is especially suitable for reverse transcription of a small number of templates and low-copy genes. In addition, the mismatch rate of HiScript® Reverse Transcriptase is also lower than that of M-MLV (RNase H-) Reverse Transcriptase, which improves the fidelity of cloning. The HiScript® First-strand cDNA Synthesis Kit is based on HiScript® Reverse Transcriptase and contains all the ingredients needed to synthesize high-quality first-strand cDNA, suitable for subsequent PCR, qPCR and PCR cloning. 2 × RT Mix contains optimized buffer system and dNTP; Enzyme Mix contains HiScript®Reverse Transcriptase and RNase inhibitor. Oligo (dT)18, Random hexamers, or gene-specific primers can be selected as reverse transcription primers according to needs. 

 

HiScript ® Reverse Transcriptase provides reliable reverse transcription performance;
A wide range of template amount, which can be successfully reverse transcribed from 10 pg-5 μg total RNA;
Efficient synthesis ability: can synthesize up to 10 kb cDNA;
Different primers can be selected for different downstream applications. The synthesized one-strand cDNA can be widely used in molecular cloning, hybridization, PCR amplification, and qPCR reactions. 

 

Store at -20℃

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R111-01/02
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