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NGS Library Prep kits

Molecular Research

Drug Discovery

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Number of views:
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Fast-T1

Fast-T1 is currently the fastest growing competent cell. Outer membrane receptor mutation (tonA) confers resistance to lytic phage T1 and T5 in competent cells; deletion of endonuclease (endA) increases the yield and quality of plasmid DNA; recombinase deficiency (recA) reduces inserts The probability of homologous recombination ensures the stability of inserted DNA; lacZΔM15 makes Fast-T1 competent cells available for blue-white screening. The company’s Fast-T1 competent cells are prepared by a special process, and the pUC19 plasmid is used to detect the transformation efficiency ≥108 cfu/μg DNA.   Fast growth: clones can be seen after 8-9 hours of culture on ampicillin-resistant plates; single clones cultured overnight in 2 ml of LB liquid medium can be cultured for 4-5 hours to extract a small amount of plasmid DNA; Compatible resistance: T1 and T5 phage resistance; Simple operation: for ampicillin-resistant plasmids and ligation products, rapid transformation can be completed in 5 minutes   Store at -70°C (do not store in -20°C or liquid nitrogen!)
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Product serial number
C505-02/03
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Fast-T1 is currently the fastest growing competent cell. Outer membrane receptor mutation (tonA) confers resistance to lytic phage T1 and T5 in competent cells; deletion of endonuclease (endA) increases the yield and quality of plasmid DNA; recombinase deficiency (recA) reduces inserts The probability of homologous recombination ensures the stability of inserted DNA; lacZΔM15 makes Fast-T1 competent cells available for blue-white screening. The company’s Fast-T1 competent cells are prepared by a special process, and the pUC19 plasmid is used to detect the transformation efficiency ≥108 cfu/μg DNA.

 

Fast growth: clones can be seen after 8-9 hours of culture on ampicillin-resistant plates; single clones cultured overnight in 2 ml of LB liquid medium can be cultured for 4-5 hours to extract a small amount of plasmid DNA;
Compatible resistance: T1 and T5 phage resistance;
Simple operation: for ampicillin-resistant plasmids and ligation products, rapid transformation can be completed in 5 minutes

 

Store at -70°C (do not store in -20°C or liquid nitrogen!)

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C505-02/03
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