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NGS Library Prep kits

Molecular Research

Drug Discovery

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Number of views:
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HiScript II U+ One Step qRT-PCR Probe Kit

The dUTP/UDG anti-contamination system is a very effective means to control the contamination of PCR amplification products. In PCR detection reagents using DNA as a template, it has gradually become a mandatory standard. However, the commonly used E. coli UDG It still has high activity at the usual reverse transcription temperature (42-55℃), which will degrade cDNA, which will reduce the sensitivity of One Step RT-PCR/qPCR. HiScript® II U+ One Step qRT-PCR Probe Kit contains an optimized ratio of dUTP/dNTP mix and heat-labile UDG derived from psychrophilic marine bacteria. Heat-labile UDG has high activity at room temperature and can fully degrade U-containing double-stranded DNA during the mixing process of the reaction system. When the reaction system is heated to 50-55°C (the optimal reaction temperature of HiScript® II), Heat-labile UDG is quickly and completely inactivated, maintaining the integrity of the cDNA, and ensuring that the detection sensitivity is not affected.    Support multiple probe detection; UDG anti-pollution system: Heat-labile UDG rapidly degrades U-containing double-stranded DNA contaminants at room temperature. Heat-labile UDG is rapidly and completely inactivated at 50-55°C to maintain the integrity of cDNA; Ultra-high detection sensitivity: single-digit copies of templates or 0.1 pg total RNA can be detected Ultra-high amplification specificity: AceTaq® DNA Polymerase based on chemical hot-start, completely blocked enzyme activity before 95°C, and equipped with a patented specific promotion factor Exactor, which makes amplification more specific;   Store at -20℃
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339.0
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$
339.0
Number of views:
4673
Product serial number
Q223-01
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The dUTP/UDG anti-contamination system is a very effective means to control the contamination of PCR amplification products. In PCR detection reagents using DNA as a template, it has gradually become a mandatory standard. However, the commonly used E. coli UDG It still has high activity at the usual reverse transcription temperature (42-55℃), which will degrade cDNA, which will reduce the sensitivity of One Step RT-PCR/qPCR.
HiScript® II U+ One Step qRT-PCR Probe Kit contains an optimized ratio of dUTP/dNTP mix and heat-labile UDG derived from psychrophilic marine bacteria. Heat-labile UDG has high activity at room temperature and can fully degrade U-containing double-stranded DNA during the mixing process of the reaction system. When the reaction system is heated to 50-55°C (the optimal reaction temperature of HiScript® II), Heat-labile UDG is quickly and completely inactivated, maintaining the integrity of the cDNA, and ensuring that the detection sensitivity is not affected. 

 

Support multiple probe detection;
UDG anti-pollution system: Heat-labile UDG rapidly degrades U-containing double-stranded DNA contaminants at room temperature. Heat-labile UDG is rapidly and completely inactivated at 50-55°C to maintain the integrity of cDNA;
Ultra-high detection sensitivity: single-digit copies of templates or 0.1 pg total RNA can be detected
Ultra-high amplification specificity: AceTaq® DNA Polymerase based on chemical hot-start, completely blocked enzyme activity before 95°C, and equipped with a patented specific promotion factor Exactor, which makes amplification more specific;

 

Store at -20℃

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