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High-Sensitive ECL Chemiluminescence Detection Kit (Ready-to-Use)

NGS Library Prep kits

Molecular Research

Drug Discovery

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Number of views:
3374

High-Sensitive ECL Chemiluminescence Detection Kit (Ready-to-Use)

This kit uses chemiluminescence method to detect protein or nucleic acid biological macromolecules. The protein or nucleic acid biological macromolecules are transferred to the blotting membrane after electrophoresis, and the primary antibody and the secondary antibody labeled with horseradish peroxidase (HRP) are used to sequentially bind the target protein on the membrane; or labeled with HRP The probe directly or indirectly binds the target nucleic acid on the membrane. After washing the membrane, incubate the membrane with freshly prepared ECL working solution for 2 min-3 min in the dark at room temperature. Cover the blot membrane with plastic wrap and fix it on the X-ray exposure cassette, then transfer it to the dark room to press the X-ray film The exposure time on the film can range from several seconds to several hours. After developing and fixing, the protein or nucleic acid bands can be clearly displayed on the X-ray film, or the X-ray film exposure and development step is not required, and the blot film can be directly scanned by CCD. This kit uses a unique luminescent substrate system, which effectively reduces the exposure background, improves the detection sensitivity, and has a significant improvement in detection durability and signal-to-noise ratio compared with the ECL enhanced luminescence kit (Vazyme #E411).   High-quality Western developing reagents; optimized formula to enhance luminous intensity; strong anti-quenching ability; compatible with film exposure and CCD scanning   Store at 2-8℃ in the dark. If not used for a long time, it can be stored at -20℃ and protected from light
Retail price
$
63.0
Market price
$
63.0
Number of views:
3374
Product serial number
E412-01/02
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+
Stock:
15096
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This kit uses chemiluminescence method to detect protein or nucleic acid biological macromolecules. The protein or nucleic acid biological macromolecules are transferred to the blotting membrane after electrophoresis, and the primary antibody and the secondary antibody labeled with horseradish peroxidase (HRP) are used to sequentially bind the target protein on the membrane; or labeled with HRP The probe directly or indirectly binds the target nucleic acid on the membrane. After washing the membrane, incubate the membrane with freshly prepared ECL working solution for 2 min-3 min in the dark at room temperature. Cover the blot membrane with plastic wrap and fix it on the X-ray exposure cassette, then transfer it to the dark room to press the X-ray film The exposure time on the film can range from several seconds to several hours. After developing and fixing, the protein or nucleic acid bands can be clearly displayed on the X-ray film, or the X-ray film exposure and development step is not required, and the blot film can be directly scanned by CCD. This kit uses a unique luminescent substrate system, which effectively reduces the exposure background, improves the detection sensitivity, and has a significant improvement in detection durability and signal-to-noise ratio compared with the ECL enhanced luminescence kit (Vazyme #E411).

 

High-quality Western developing reagents; optimized formula to enhance luminous intensity; strong anti-quenching ability; compatible with film exposure and CCD scanning

 

Store at 2-8℃ in the dark. If not used for a long time, it can be stored at -20℃ and protected from light

Keyword:
E412-01/02
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  • E412-High-Sensitive ECL Chemiluminescence Detection Kit (Ready-to-Use).pdf

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