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NGS Library Prep kits

Molecular Research

Drug Discovery

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Number of views:
2476

Plant Direct PCR Kit

This product is suitable for direct amplification of plant leaves, seeds, etc., and can be used for high-throughput screening of non-polysaccharide and polyphenol plant samples. Direct-expanded DNA polymerase modified by directed evolution has strong tolerance to PCR inhibitors in plants, while maintaining high amplification performance, suitable for the amplification of DNA fragments within 5 kb; in the kit The unique lysis buffer A can be used to lyse fresh or frozen plant tissues. The operation is simple, and the resulting lysate can be used as an amplification template without purification. The protective agent added to the lysate allows the crude product to be effectively amplified after multiple freeze-thaw cycles. The pre-configured 2 × Plant Direct Master Mix only needs to add primers and templates to perform amplification reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. The amplified products are blunt-ended and are suitable for ClonExpress® and TOPOTM rapid cloning kits (C112/C113/C115/C601).   Unique super-resistant DNA polymerase: Direct-expanded DNA polymerase modified by directed evolution has strong tolerance to PCR inhibitors in plants, while maintaining extremely high amplification performance; Broad sample compatibility: suitable for the amplification of various common plant leaves and crude seeds; Very high stability: 50 times of repeated freezing and thawing, the activity has not been significantly reduced; Convenient operation: Without complicated DNA extraction steps, PCR can be performed using a few leaves or simple lysis, saving a lot of time.    2× Plant Direct Master Mix can be stored at -20℃; Plant Direct Lysis Buffer can be stored at -20℃ or 2-8℃
Retail price
$
600.0
Market price
$
0.0
Number of views:
2476
Product serial number
PD105-01/02
Category
Direct PCR
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Stock:
3996
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This product is suitable for direct amplification of plant leaves, seeds, etc., and can be used for high-throughput screening of non-polysaccharide and polyphenol plant samples. Direct-expanded DNA polymerase modified by directed evolution has strong tolerance to PCR inhibitors in plants, while maintaining high amplification performance, suitable for the amplification of DNA fragments within 5 kb; in the kit The unique lysis buffer A can be used to lyse fresh or frozen plant tissues. The operation is simple, and the resulting lysate can be used as an amplification template without purification. The protective agent added to the lysate allows the crude product to be effectively amplified after multiple freeze-thaw cycles. The pre-configured 2 × Plant Direct Master Mix only needs to add primers and templates to perform amplification reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. The amplified products are blunt-ended and are suitable for ClonExpress® and TOPOTM rapid cloning kits (C112/C113/C115/C601).

 

Unique super-resistant DNA polymerase: Direct-expanded DNA polymerase modified by directed evolution has strong tolerance to PCR inhibitors in plants, while maintaining extremely high amplification performance;
Broad sample compatibility: suitable for the amplification of various common plant leaves and crude seeds;
Very high stability: 50 times of repeated freezing and thawing, the activity has not been significantly reduced;
Convenient operation: Without complicated DNA extraction steps, PCR can be performed using a few leaves or simple lysis, saving a lot of time. 

 

2× Plant Direct Master Mix can be stored at -20℃; Plant Direct Lysis Buffer can be stored at -20℃ or 2-8℃

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