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Hyperactive In-Situ ChIP Library Prep Kit for Illumina(pG-Tn5)

NGS Library Prep kits

Molecular Research

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Hyperactive In-Situ ChIP Library Prep Kit for Illumina(pG-Tn5)

HyperactiveTM In-Situ ChIP Library Prep Kit for Illumina is a dedicated kit developed for CUT&Tag technology. CUT&Tag technology is a new method for studying protein-DNA interactions. By fusing Protein G or Protein A with the engineered ultra-highly active Tn5 transposase, a new fusion enzyme with dual activity (Hyperactive pG- Tn5/pA-Tn5 Transposase), under the guidance of the antibody, accurately target and cut the DNA sequence near the target protein. Compared with traditional ChIP-Seq, this technology has the advantages of low cell input, short experiment cycle, high signal-to-noise ratio, and good repeatability. It is especially suitable for early embryonic development, stem cells, tumors, and epigenetics. field. All reagents in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.   Simple operation: DNA fragmentation and adaptor ligation can be realized in one step, and the transformation from cell to next-generation sequencing library can be realized in only 9 hours Super high activity: both Protein A&Tn5 activity, extremely high DNA fragmentation activity Low initial cell volume: compatible with as few as 60 cells, even single cells High purity: high protein purity, low nucleic acid residue   BOX 1: ConA beads are stored at 2 ~ 8℃, and the remaining components are stored at room temperature. BOX 2: 5% Digitonin stored at -30 ~ -15℃, and can be stored at room temperature for 1 week; 10 × Binding Buffer -30 ~ -15 ℃ storage, 2 ~ 8 ℃ can be stored for 6 months; Store the remaining components at -30 ~ -15°C.  
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$
1124.0
Market price
$
1124.0
Number of views:
3812
Product serial number
TD901-01/02
Category
CUT&Tag
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HyperactiveTM In-Situ ChIP Library Prep Kit for Illumina is a dedicated kit developed for CUT&Tag technology. CUT&Tag technology is a new method for studying protein-DNA interactions. By fusing Protein G or Protein A with the engineered ultra-highly active Tn5 transposase, a new fusion enzyme with dual activity (Hyperactive pG- Tn5/pA-Tn5 Transposase), under the guidance of the antibody, accurately target and cut the DNA sequence near the target protein. Compared with traditional ChIP-Seq, this technology has the advantages of low cell input, short experiment cycle, high signal-to-noise ratio, and good repeatability. It is especially suitable for early embryonic development, stem cells, tumors, and epigenetics. field. All reagents in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.

 

Simple operation: DNA fragmentation and adaptor ligation can be realized in one step, and the transformation from cell to next-generation sequencing library can be realized in only 9 hours
Super high activity: both Protein A&Tn5 activity, extremely high DNA fragmentation activity
Low initial cell volume: compatible with as few as 60 cells, even single cells
High purity: high protein purity, low nucleic acid residue
 

BOX 1: ConA beads are stored at 2 ~ 8℃, and the remaining components are stored at room temperature.
BOX 2: 5% Digitonin stored at -30 ~ -15℃, and can be stored at room temperature for 1 week;
10 × Binding Buffer -30 ~ -15 ℃ storage, 2 ~ 8 ℃ can be stored for 6 months;
Store the remaining components at -30 ~ -15°C.
 

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  • TD901 TD902 V9.1 Hyperactive In-Situ ChIP Library Prep Kit for Illumina.pdf

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