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NGS Library Prep kits

Molecular Research

Drug Discovery

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Hyperactive pA-MNase for CUT&RUN

 Hyperactive pA-MNase for CUT&RUN is the fusion of Protein A and engineered ultra-highly active MNase to form a new fusion enzyme with both MNase exonuclease activity and Protein A activity, which is specifically suitable for protein-genome interaction research CUT&RUN technology. Compared with the traditional protein-genome interaction research method ChIP-Seq, CUT&RUN has significant advantages. This technology has a short experimental cycle, high signal-to-noise ratio, good repeatability, and low cell input. It is especially suitable for early embryo development, Research fields such as stem cells, tumors and epigenetics.   (1) Simple and fast workflow: DNA denaturation and bisulfite conversion are completed in one step, and the conversion reaction time is only 140 min (2) High yield: 10 pg-100 ng genomic DNA starting amount, recovery efficiency ≥ 80%, conversion efficiency of unmethylated cytosine ≥ 99% (3) Suitable for various downstream applications: Bisulfite-converted DNA can be used for stable PCR amplification for downstream analysis of NGS sequencing, etc. (4) Compatible with DNA templates from different sources: DNA extracted from cells or tissues of animals, plants, microorganisms; cfDNA; purified PCR products, etc.   Store at -30 ~ -15℃, and transport at -20 ~ 0℃.    
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Number of views:
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Product serial number
S701-01/02
Category
CUT&Tag
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 Hyperactive pA-MNase for CUT&RUN is the fusion of Protein A and engineered ultra-highly active MNase to form a new fusion enzyme with both MNase exonuclease activity and Protein A activity, which is specifically suitable for protein-genome interaction research CUT&RUN technology. Compared with the traditional protein-genome interaction research method ChIP-Seq, CUT&RUN has significant advantages. This technology has a short experimental cycle, high signal-to-noise ratio, good repeatability, and low cell input. It is especially suitable for early embryo development, Research fields such as stem cells, tumors and epigenetics.

 

(1) Simple and fast workflow: DNA denaturation and bisulfite conversion are completed in one step, and the conversion reaction time is only 140 min
(2) High yield: 10 pg-100 ng genomic DNA starting amount, recovery efficiency ≥ 80%, conversion efficiency of unmethylated cytosine ≥ 99%
(3) Suitable for various downstream applications: Bisulfite-converted DNA can be used for stable PCR amplification for downstream analysis of NGS sequencing, etc.
(4) Compatible with DNA templates from different sources: DNA extracted from cells or tissues of animals, plants, microorganisms; cfDNA; purified PCR products, etc.
 

Store at -30 ~ -15℃, and transport at -20 ~ 0℃.

 

 

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