The dUTP/UDG anti-contamination system is a very effective means to control the contamination of PCR amplification products. In PCR detection reagents using DNA as a template, it has gradually become a mandatory standard. However, the commonly used E. coli UDG It still has high activity at the usual reverse transcription temperature (42-55℃), which will degrade cDNA, which will reduce the sensitivity of One Step RT-PCR/qPCR.
HiScript® II U+ One Step qRT-PCR Probe Kit contains an optimized ratio of dUTP/dNTP mix and heat-labile UDG derived from psychrophilic marine bacteria. Heat-labile UDG has high activity at room temperature and can fully degrade U-containing double-stranded DNA during the mixing process of the reaction system. When the reaction system is heated to 50-55°C (the optimal reaction temperature of HiScript® II), Heat-labile UDG is quickly and completely inactivated, maintaining the integrity of the cDNA, and ensuring that the detection sensitivity is not affected.
Support multiple probe detection;
UDG anti-pollution system: Heat-labile UDG rapidly degrades U-containing double-stranded DNA contaminants at room temperature. Heat-labile UDG is rapidly and completely inactivated at 50-55°C to maintain the integrity of cDNA;
Ultra-high detection sensitivity: single-digit copies of templates or 0.1 pg total RNA can be detected
Ultra-high amplification specificity: AceTaq® DNA Polymerase based on chemical hot-start, completely blocked enzyme activity before 95°C, and equipped with a patented specific promotion factor Exactor, which makes amplification more specific;
Store at -20℃
The detection rate of low copy number templates or weak positive samples is still one of the challenges of clinical diagnosis. HiScript® II One Step qRT-PCR Probe Kit contains the best ratio of HiScript® II Reverse Transcriptase and Champagne TaqTM DNA Polymerase, as well as a fully optimized buffer system, whether it is total RNA, in vitro transcription RNA, or viral RNA, HiScript® II The One Step qRT-PCR Probe Kit has extremely high sensitivity and provides stable and reliable amplification performance. Without too much optimization, multiple amplification can be achieved.
Ultra-high detection sensitivity: single-digit copies of templates or 0.1 pg total RNA can be detected;
Multiple detection: support multiple probe detection
Store at -20℃
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HiScript® II One Step qRT-PCR Probe Kit 以RNA为模板的探针法qRT-PCR试剂盒
HiScript® II One Step qRT-PCR SYBR® Green Kit uses the SYBR® Green I chimeric fluorescence method and is designed for quantitative PCR detection using RNA as a template (such as RNA viruses). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. Integrating the superior performance of HiScript® II Reverse Transcriptase and Champagne TaqTM DNA Polymerase, with an optimized buffer system, the detection sensitivity of the One Step qRT-PCR SYBR® Green Kit can reach 1 pg of total RNA. The enhancement factor added to the buffer can effectively reduce the formation of primer dimers and improve specificity. The kit is provided in a convenient Master Mix format. 2 × One Step SYBR® Green Mix contains optimized buffer system, dNTP, specific enhancement factor and SYBR® Green I fluorescent dye; One Step SYBR® Green Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM DNA Polymerase
Excellent specificity: special additive components can minimize the generation of primer dimers, and the quantitative results are more accurate;
Ultra-high detection sensitivity: taking into account high amplification efficiency, it can detect as little as 1 pg of total RNA template
Store at -20°C. 2 × One Step SYBR® Green Mix Store at -20℃ and avoid light
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HiScript® II One Step qRT-PCR SYBR® Green Kit 以RNA为模板的染料法qRT-PCR试剂盒
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