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NGS Library Prep kits

Molecular Research

Drug Discovery

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T4 Polynucleotide Kinase

T4 Polynucleotide Kinase can catalyze the transfer of the γ-phosphate group of ATP to the 5´-hydroxyl end and 3´-monophosphate nucleoside of the oligonucleotide chain (double-stranded or single-stranded DNA or RNA). T4 Polynucleotide Kinase also has 3´-phosphatase activity, which hydrolyzes 3´-phosphate groups from the 3´ phosphate ends of oligonucleotides, deoxy 3´-monophosphate nucleosides and deoxy 3´-diphosphate nucleosides .   Prepare probes with random primers; Cut off the 3′ protruding end or fill in the 5′ protruding end to form a flat end   Store at -20℃
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T4 Polynucleotide Kinase can catalyze the transfer of the γ-phosphate group of ATP to the 5´-hydroxyl end and 3´-monophosphate nucleoside of the oligonucleotide chain (double-stranded or single-stranded DNA or RNA). T4 Polynucleotide Kinase also has 3´-phosphatase activity, which hydrolyzes 3´-phosphate groups from the 3´ phosphate ends of oligonucleotides, deoxy 3´-monophosphate nucleosides and deoxy 3´-diphosphate nucleosides .

 

Prepare probes with random primers;
Cut off the 3′ protruding end or fill in the 5′ protruding end to form a flat end
 

Store at -20℃

Keyword:
t4
the
of
end
or
phosphate
polynucleotide
kinase
nucleosides
deoxy
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