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NGS Library Prep kits

Molecular Research

Drug Discovery

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Discover-sc Single Cell Kit V2

Discover-scTM Single Cell Kit V2 uses an MDA-based isothermal amplification system, which can use a single cell or a small sample as a template to realize the undifferentiated amplification of the whole genome. The single-cell genome can reach more than 95% coverage after amplification The size of the amplified product is between 2-100 kb, and the average product length is greater than 20 kb. It can be widely used in whole genome and exome sequencing, large fragment copy number variation analysis, microsatellite analysis, qPCR analysis, and gene chip analysis. The Phi29 DNA polymerase used in this kit is a DNA polymerase cloned from phage. It has strong strand displacement activity and can realize the melting and replication of complex structures of DNA; and Phi29 DNA polymerase has strong strand affinity , A single polymerization reaction can achieve continuous polymerization extension up to 100 kb, and its amplified products are suitable for almost all downstream applications; Phi29 DNA polymerase also has strong 3'-5' exonuclease activity, and its fidelity The degree is 1000 times that of Taq enzyme, which guarantees the high fidelity of DNA synthesis. Under normal circumstances, Discover-scTM Single Cell Kit V2 can generate 30-40 μg of genomic DNA in one reaction.    Wide sample compatibility: suitable for samples of animal and plant cells, bacteria or blastomeres, nourishing outer embryonic layer cells, sperm, etc. High coverage: the single-cell genome can reach more than 95% coverage after amplification High uniformity: can be used for analysis of chromosome copy number variation >1 Mb High fidelity: The fidelity of Phi29 DNA polymerase is 1000 times that of Taq enzyme Simple operation: single-tube reaction, operation time is less than 10 min, no need to purify the amplified product   Box1: Store at -20°C, please store below -70°C for longer storage; Box2: Store at -20°C away from light;
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Number of views:
2252
Product serial number
N602-02
Category
WGA
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Discover-scTM Single Cell Kit V2 uses an MDA-based isothermal amplification system, which can use a single cell or a small sample as a template to realize the undifferentiated amplification of the whole genome. The single-cell genome can reach more than 95% coverage after amplification The size of the amplified product is between 2-100 kb, and the average product length is greater than 20 kb. It can be widely used in whole genome and exome sequencing, large fragment copy number variation analysis, microsatellite analysis, qPCR analysis, and gene chip analysis.
The Phi29 DNA polymerase used in this kit is a DNA polymerase cloned from phage. It has strong strand displacement activity and can realize the melting and replication of complex structures of DNA; and Phi29 DNA polymerase has strong strand affinity , A single polymerization reaction can achieve continuous polymerization extension up to 100 kb, and its amplified products are suitable for almost all downstream applications; Phi29 DNA polymerase also has strong 3'-5' exonuclease activity, and its fidelity The degree is 1000 times that of Taq enzyme, which guarantees the high fidelity of DNA synthesis. Under normal circumstances, Discover-scTM Single Cell Kit V2 can generate 30-40 μg of genomic DNA in one reaction. 

 

Wide sample compatibility: suitable for samples of animal and plant cells, bacteria or blastomeres, nourishing outer embryonic layer cells, sperm, etc.
High coverage: the single-cell genome can reach more than 95% coverage after amplification
High uniformity: can be used for analysis of chromosome copy number variation >1 Mb
High fidelity: The fidelity of Phi29 DNA polymerase is 1000 times that of Taq enzyme
Simple operation: single-tube reaction, operation time is less than 10 min, no need to purify the amplified product
 

Box1: Store at -20°C, please store below -70°C for longer storage;
Box2: Store at -20°C away from light;

We could not find any corresponding parameters, please add them to the properties table

 

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