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VAHTS Stranded mRNA-seq Library Prep Kit for Illumina

NGS Library Prep kits

Molecular Research

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VAHTS Stranded mRNA-seq Library Prep Kit for Illumina

VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina® is a special kit for transcriptome library construction targeted for the Illumina® high-throughput sequencing platform. This kit is suitable for constructing a starting template with an input amount of 0.1-4 μg total RNA A chain-specific transcriptome library of eukaryotes such as animals, plants, and fungi. The difference from conventional transcriptome library construction is that the total RNA of the sample is separated, fragmented, and first-strand cDNA is synthesized. During synthesis, dUTP was incorporated to label it, and at the same time, the end repair was completed at this step; followed by the steps of adding A-tailing, ligation adapter, ligation product purification, fragment size sorting, library amplification, etc., before PCR enrichment of the library The U-labeled second-strand template is digested with UDG enzyme. Therefore, the final sequencing information comes from the first-strand cDNA, which preserves the strand directionality of the mRNA. Data analysis after sequencing can determine whether the transcript comes from the sense strand or Antisense DNA strand. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet different needs. The kit includes three independent packages of NR1, NR5 and NR6, which contain all the enzymes and buffers needed for the library construction process. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the library to the greatest extent Stability and repeatability of the construction.    Includes Ribo-OffTM rRNA depletion module, which can remove ribosomal RNA from human, mouse, rat, cow, dog, horse, chicken, monkey, pig, zebrafish and other species; Chain-specific library construction; Optimized large fragment library construction scheme   NR 1, 2-8°C storage; NR 5, stored at -20°C; NR 6, stored at -20°C. 
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VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina® is a special kit for transcriptome library construction targeted for the Illumina® high-throughput sequencing platform. This kit is suitable for constructing a starting template with an input amount of 0.1-4 μg total RNA A chain-specific transcriptome library of eukaryotes such as animals, plants, and fungi. The difference from conventional transcriptome library construction is that the total RNA of the sample is separated, fragmented, and first-strand cDNA is synthesized. During synthesis, dUTP was incorporated to label it, and at the same time, the end repair was completed at this step; followed by the steps of adding A-tailing, ligation adapter, ligation product purification, fragment size sorting, library amplification, etc., before PCR enrichment of the library The U-labeled second-strand template is digested with UDG enzyme. Therefore, the final sequencing information comes from the first-strand cDNA, which preserves the strand directionality of the mRNA. Data analysis after sequencing can determine whether the transcript comes from the sense strand or Antisense DNA strand.
This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet different needs. The kit includes three independent packages of NR1, NR5 and NR6, which contain all the enzymes and buffers needed for the library construction process. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the library to the greatest extent Stability and repeatability of the construction. 

 

Includes Ribo-OffTM rRNA depletion module, which can remove ribosomal RNA from human, mouse, rat, cow, dog, horse, chicken, monkey, pig, zebrafish and other species;
Chain-specific library construction;
Optimized large fragment library construction scheme
 

NR 1, 2-8°C storage;
NR 5, stored at -20°C;
NR 6, stored at -20°C. 

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