RoomTemp TM Sample Lysis Kit is a simple and fast blood lysis kit at room temperature. This kit contains two components, Lysis Buffer and Stabilizing Buffer. Lysis Buffer contains special components that quickly destroy cell membrane proteins and membrane structure, which can fully release the genomic DNA in the cell. Stabilizing Buffer adds protective proteins and stabilizing factors. Eliminate the inhibitory effect of inhibitors in the lysed sample on downstream qPCR and PCR reactions, so that the lysed DNA solution can be stored stably for a long time. Applicable blood sample types include fresh blood, frozen blood and conventional anticoagulant blood (EDTA, citrate, heparin sodium, etc.). After lysis at room temperature for 3 minutes, genomic DNA can be released from the whole blood sample. The lysed DNA solution can be directly used as a template for SNP detection by Taqman ® probe method, qPCR probe method quantification, PCR amplification, etc. Without complicated extraction operations, it can achieve the same effect as traditional genome extraction methods. In addition to blood, this kit is also compatible with pyrolysis FTA blood cards, buccal swabs, plant tissues and other samples.
Simple operation: 3 minutes at room temperature, no need for complex template extraction.
High lysis efficiency: The lysis reagent has the same effect as traditional kits for extracting genome.
Wide range of applications: Compatible with Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification and other sample compatibility: lysis of fresh blood, frozen blood, conventional anticoagulant blood, tissue homogenate, oral swabs, Various samples such as FTA card.
Store at 2~8℃.
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