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这是描述信息

NGS Library Prep kits

Molecular Research

Drug Discovery

One Step RT-PCR

1200.00
Article number:
HF001-1 
HiscriptHighFidelityOneStepRT-PCRKit一步法高效RT-PCR试剂盒逆转录和PCR在同一管内进行检测灵敏度可达0.1pg总RNA可扩增超过10kb的长片段图1A.以Hela细胞总RNA为模板,用Hiscript® HighFidelityOneStepRT-PCRKit (HF001)扩增555bpGAPDH片段。检测灵敏度可达到0.1pg。B.以猪蓝耳病毒PRRSVRNA为模板,用Hiscript®HighFidelityOneStepRT-PCRKit(HF001)扩增660bp片段。检测灵敏度可达到0.2TCLD50。C.以1μgHela细胞总RNA为模板,用Hiscript®HighFidelityOneStepRT-PCRKit(HF001)扩增不同长度的片段。可成功扩增长达12.1kb的UTRN片段。本试剂盒专为以RNA为模板(如RNA病毒)的终点法PCR检测而设计。使用基因特异引物(GSP),逆转录和PCR反应在一管内完成,不需要额外的开管/移液操作,大大提高了检测通量,并降低了污染的风险。整合HiScript®IIReverseTranscriptase以及ChampagneTaqTMDNAPolymerase的优越性能,配合经过优化的缓冲体系,Hiscript®HighFidelityOneStepRT-PCRKit(HF001) 可扩增片段长达10kb以上。试剂盒以便捷的MasterMix形式提供。2×OneStepMix包含优化的缓冲体系和dNTPs;OneStepEnzymeMix包含比例优化的HiScript®IIReverseTranscriptase、RNaseinhibitor以及ChampagneTaqTM plusDNAPolymerase,操作简单快捷。组分HF001-0150rxn(50ul/rxn)RNasefreeddH2O1ml×22×OneStepMixa625μl×2OneStepEnzymeMixb125μl10×Loadingbuffer1.25mla.包含dNTP和缓冲体系b.包含HiScript® IIReverseTranscriptase、RNaseinhibitor以及ChampagneTaqTM plusDNAPolymerase-30~-15℃保存。
1200.00
Article number:
HF001-1 
Number of views:
4965
Keywords:
fidelity
one
step
hiscript
片段
rt-pcr
扩增
rna
kit
检测
HF001-01
HF001
hf001
119.00
Article number:
P612-01
HiScript® II One Step RT-PCR Kit (Dye Plus) is specially designed for end-point PCR detection using RNA as a template (such as RNA virus). PCR products can be directly loaded for electrophoresis, which greatly saves time and labor. Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which improves detection throughput and reduces the risk of contamination. Integrating the superior performance of HiScript® II Reverse Transcriptase and Champagne TaqTM plus DNA Polymerase, with an optimized buffer system, the detection sensitivity of HiScript® II One Step RT-PCR Kit (Dye Plus) can reach 1 pg total RNA and can be amplified Fragments up to 10 kb. The kit is provided in a convenient Master Mix format. 2 × One Step Mix (Dye Plus) contains optimized buffer system, dNTP and loading dye; One Step Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.   Reverse transcription and PCR are performed in the same tube; The detection sensitivity can reach 0.1 pg total RNA; Amplify long fragments up to 10 kb or more; PCR products can be directly loaded for electrophoresis   Store at -20℃
119.00
Article number:
P612-01
Number of views:
4855
Keywords:
P612-01
119.00
Article number:
P611-01
HiScript® II One Step RT-PCR Kit is specially designed for end-point PCR detection using RNA as template (such as RNA virus). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. Integrate HiScript® II Reverse Transcriptase and the superior performance of Champagne TaqTM plus DNA Polymerase designed for long fragment amplification, and cooperate with the optimized buffer system HiScript® II One Step RT-PCR Kit to amplify fragments up to 10 kb or more. The kit is provided in a convenient Master Mix format. 2 × One Step Mix contains optimized buffer system and dNTP; One Step Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.   Reverse transcription and PCR are performed in the same tube; The detection sensitivity can reach 0.1 pg total RNA; Amplify long fragments over 10 kb   Store at -20℃
119.00
Article number:
P611-01
Number of views:
4995
Keywords:
P611-01

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这是描述信息

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