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NGS Library Prep kits

Molecular Research

Drug Discovery

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Number of views:
2832

Discover-sc Single Cell Kit

Discover-scTM Single Cell Kit uses an isothermal amplification system based on MDA, which can use a single cell or a small sample as a template to achieve indiscriminate amplification of the whole genome. The single cell genome can reach a coverage of more than 95% after amplification. The size of the amplified product is between 2-100 kb, and the average product length is greater than 20 kb. It can be widely used in whole genome and exome sequencing, large fragment copy number variation analysis, microsatellite analysis, qPCR analysis, and gene chip analysis. The Phi29 DNA polymerase used in this kit is a DNA polymerase cloned from phage. It has strong strand displacement activity and can realize the melting and replication of complex structures of DNA; and Phi29 DNA polymerase has strong strand affinity , A single polymerization reaction can achieve continuous polymerization extension up to 100 kb, and its amplified products are suitable for almost all downstream applications; Phi29 DNA polymerase also has strong 3'-5' exonuclease activity, and its fidelity The degree is 1000 times that of Taq enzyme, which guarantees the high fidelity of DNA synthesis. Under normal circumstances, one reaction of Discover-scTM Single Cell Kit can produce 30-40 μg of genomic DNA with high coverage.    High sensitivity; High coverage High uniformity High fidelity high production    Store at -20℃. For longer-term storage, please store below -70℃.
Retail price
$
365.0
Market price
$
365.0
Number of views:
2832
Product serial number
N601-01/02
Category
WGA
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Stock:
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Discover-scTM Single Cell Kit uses an isothermal amplification system based on MDA, which can use a single cell or a small sample as a template to achieve indiscriminate amplification of the whole genome. The single cell genome can reach a coverage of more than 95% after amplification. The size of the amplified product is between 2-100 kb, and the average product length is greater than 20 kb. It can be widely used in whole genome and exome sequencing, large fragment copy number variation analysis, microsatellite analysis, qPCR analysis, and gene chip analysis.
The Phi29 DNA polymerase used in this kit is a DNA polymerase cloned from phage. It has strong strand displacement activity and can realize the melting and replication of complex structures of DNA; and Phi29 DNA polymerase has strong strand affinity , A single polymerization reaction can achieve continuous polymerization extension up to 100 kb, and its amplified products are suitable for almost all downstream applications; Phi29 DNA polymerase also has strong 3'-5' exonuclease activity, and its fidelity The degree is 1000 times that of Taq enzyme, which guarantees the high fidelity of DNA synthesis. Under normal circumstances, one reaction of Discover-scTM Single Cell Kit can produce 30-40 μg of genomic DNA with high coverage. 

 

High sensitivity;
High coverage
High uniformity
High fidelity
high production

 

 Store at -20℃. For longer-term storage, please store below -70℃.

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