LOGO
Search
Submit
Cancel
这是描述信息

NGS Library Prep kits

Molecular Research

Drug Discovery

RT-qPCR SuperMix

242.00
Article number:
R323-01
HiScript® III RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript® II Q RT SuperMix for qPCR (+gDNA wiper), including a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the optimal buffer optimized for reverse transcription further improve the efficiency of cDNA synthesis and are suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the kit can completely remove the remaining genomic DNA in the RNA template, ensure that the subsequent quantitative results are more reliable, and simplify the design of qPCR primers without the need to design primers across introns; 5 × HiScript III qRT SuperMix contains All components required for reverse transcription reaction can be reacted quickly by adding template RNA and water, and at the same time, the gDNA wiper function is terminated to ensure the integrity of cDNA. The reverse transcription product is compatible with dye method and probe method qPCR, which can perform high-performance gene expression analysis.    Easy and quick operation: 5 × HiScript III qRT SuperMix contains all the components required for reverse transcription reaction, only need to add template RNA, the reverse transcription reaction can be completed within 20 minutes; Extensive template compatibility: Compatible with RNA templates of different species and poor integrity; Super impurity tolerance: It has super tolerance to common reverse transcription impurities such as ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid, etc.; Excellent reverse transcription efficiency: Compared with common commercial reverse transcription products, HiScript ® III RT SuperMix for qPCR (+gDNA wiper) reverses the smallest cDNA quantitative C T value and has excellent reverse transcription efficiency.   Store at -30~-15°C
242.00
Article number:
R323-01
Number of views:
13662
Keywords:
gdna
the
reverse
transcription
and
for
hiscript
of
supermix
iii
180.00
Article number:
R233-01
This product is based on the cost-effective HiScript ® II Reverse Transcriptase and uses uniquely designed Oligo(dT) 23 VN primers to improve the specificity and sensitivity of the reaction and obtain better quality cDNA. Different types of reverse transcription primers can be used flexibly for different experimental designs to meet diverse experimental needs. Oligo (dT) 23 VN primer/Random Hexamers or gene-specific primers (GSP) can be selected according to the needs of subsequent reverse transcription experiments. At the same time, the reaction system is greatly simplified. 5 × qRT SuperMix contains all the reagents required for reverse transcription reaction except the primers and will not freeze at -20°C, making it easy to use. The 4 × gDNA wiper Mix in the kit can completely remove residual genomic DNA contamination, ensuring that the subsequent quantitative results are more reliable. The product obtained by reverse transcription can be used in SYBR ® Green qPCR analysis method and probe analysis method for high-performance gene expression analysis.   Based on the efficient HiScript ® II Reverse Transcriptase; Different types of reverse transcription primers can be used flexibly for different experimental designs; gDNA wiper Mix can quickly and completely remove genome contamination   Store at -20℃
180.00
Article number:
R233-01
Number of views:
5377
Keywords:
R233-01
169.00
Article number:
R232-01
HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Greatly improve thermal stability and cDNA synthesis efficiency. HiScript® II Q Select RT SuperMix for qPCR is suitable for two-step qRT-PCR detection. 5 × HiScript II Select qRT SuperMix contains Buffer, dNTP Mix, HiScript II Reverse Transcriptase and RNase inhibitor. Oligo (dT) can be selected as required 23VN, Random primers or gene-specific primers are used as reverse transcription primers. Oligo (dT)23VN has stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. 5 × HiScript® II Select qRT SuperMix will not freeze at -20℃, and the reaction can be performed quickly by adding template RNA and primers. The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for use in conjunction with high-performance gene expression analysis.    Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher; Different types of reverse transcription primers can be used flexibly for different experimental designs; Ready-to-use SuperMix premix is ​​easy to use and time-saving   Store at -20℃
169.00
Article number:
R232-01
Number of views:
4421
Keywords:
R232-01
290.00
Article number:
R223-01
HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Greatly improve thermal stability and cDNA synthesis efficiency. HiScript® II Q RT SuperMix for qPCR (+gDNA wiper) is suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the RNA template product can completely remove residual genomic DNA contamination, ensuring that the subsequent quantitative results are more reliable. 5 × HiScript qRT SuperMix II contains all the components required for the reverse transcription reaction. Add template RNA and water to quickly react and terminate the gDNA wiper function to ensure the integrity of the cDNA. This product is specially optimized for qPCR. The optimized ratio of Random primers/Oligo(dT)23VN primer mix enables cDNA synthesis to start from various regions of the RNA transcript and has the same reverse transcription efficiency, ensuring the maximum qPCR results Authenticity and repeatability. The reverse transcription product is compatible with SYBR Green and probe qPCR, and the corresponding reagents can be selected according to the purpose of the experiment to perform high-performance gene expression analysis.    Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher; Anchored Oligo (dT) 23 VN is uniquely designed to ensure reaction specificity and improve the efficiency and success rate of first-strand cDNA synthesis; The ready-to-use SuperMix premix is ​​convenient and time-saving to use; gDNA wiper Mix can quickly and completely remove genome contamination   Store at -20℃
290.00
Article number:
R223-01
Number of views:
12661
Keywords:
169.00
Article number:
R222-01
HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology based on M-MLV (RNase H-) Reverse Transcriptase, which greatly improves thermal stability and cDNA synthesis efficiency. HiScript® II Q RT SuperMix for qPCR is suitable for two-step qRT-PCR detection. 5 × HiScript II qRT SuperMix contains all the components required for reverse transcription reaction. The reaction can be performed quickly by adding template RNA and water. The volume of RNA template can be added up to the total volume It is very suitable for the reverse transcription reaction of low-concentration RNA template. 5 × HiScript® II qRT SuperMix will not freeze at -20°C and is easy to use. This product is specially optimized for qPCR. Oligo (dT)23VN has stronger anchoring ability to Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. The optimized ratio of Random primers/Oligo (dT)23VN primer mix enables cDNA synthesis to start from each region of the RNA transcript and has the same reverse transcription efficiency, ensuring the authenticity and repeatability of qPCR results to the greatest extent. The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for use in conjunction with high-performance gene expression analysis.    Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher; Anchored Oligo (dT)23 VN is uniquely designed to ensure reaction specificity and improve the efficiency and success rate of first-strand cDNA synthesis; The ready-to-use SuperMix is ​​easy to use and time-saving   Store at -20℃
169.00
Article number:
R222-01
Number of views:
5380
Keywords:
9999.00
Article number:
R122-01
HiScript® Reverse Transcriptase is a new reverse transcriptase derived from M-MLV (RNase H-) Reverse Transcriptase through multiple mutations, which improves thermal stability and cDNA synthesis efficiency. HiScript® Q RT SuperMix for qPCR is suitable for both Step qRT-PCR detection, 5 × qRT SuperMix contains all the components required for reverse transcription reaction, and the reaction can be carried out quickly by adding template RNA and water. The volume of RNA template can be added up to 80% of the total volume, which is very suitable Reverse transcription reaction for low-concentration RNA template. 5×Mix will not freeze at -20°C and is easy to use. This product is specially optimized for qPCR. The optimized ratio of Random primers/Oligo dT primer mix enables cDNA synthesis to start from each region of the RNA transcript and has the same reverse transcription efficiency, ensuring the authenticity of the qPCR results to the greatest extent. Repeatability. The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for use in conjunction with high-performance gene expression analysis.    Ready-to-use SuperMix premix is ​​convenient and time-saving   Store at -20℃
9999.00
Article number:
R122-01
Number of views:
3974
Keywords:
R122-01

The products on this website may not be sold in all countries or regions. Please contact Vazyme or your local distributor for further information.

这是描述信息

Add:Nanjing State Economy & Technology Development Zone, Red Maple Technology Industrial Park, building C 1-2 Tel:400-600-9335
Copyright 2015 Vazyme Biotech Co.,Ltd All rights reserved 
苏ICP备12014779号  Power by: www.300.cn nanjing.300.cn