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NGS Library Prep kits

Molecular Research

Drug Discovery

Conventional RT-PCR

174.00
Article number:
R312-01/02
HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the most suitable for reverse transcription optimization. Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across introns. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR quantification.   Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product; Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs; gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns    Store at -30~-15°C (restore 10*RT Mix to room temperature and shake well for use)
174.00
Article number:
R312-01/02
Number of views:
8935
Keywords:
synthesis
the
reverse
primers
for
and
of
to
can
transcription
144.00
Article number:
R302-01
HiScript® III Reverse Transcriptase is an upgraded version of HiScript® II Reverse Transcriptase, which can perform highly efficient reverse transcription reactions at 37°C. HiScript® III Reverse Transcriptase still retains the thermal stability of the second-generation product HiScript® II Reverse Transcriptase. For RNA with complex secondary structure, the reverse transcription temperature can be increased To 50~55℃, avoid the inhibition of cDNA synthesis by RNA complex secondary structure, and can effectively synthesize high-quality cDNA. In addition, this product still has superior continuous synthesis ability and super Strong impurity tolerance.    Extensive template compatibility: compatible with various templates such as animals, plants, viruses, etc.; Super impurity tolerance: It has super tolerance to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid); Excellent reverse transcription efficiency: reverse transcription efficiency is higher than that of second-generation products    Store at -30~-15°C    
144.00
Article number:
R302-01
Number of views:
3986
Keywords:
hiscript
reverse
transcription
of
the
can
transcriptase
super
impurity
20.00
Article number:
R301-01/02/03
Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript® II Reverse Transcriptase, HiScript® Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).   The enhanced antioxidant capacity will not form the disulfide bonds that human-derived proteins would produce under oxidative conditions; High-purity E. coli soluble expression, no RNase residue, compatible with RT-PCR/qPCR system   Store at -20℃
20.00
Article number:
R301-01/02/03
Number of views:
3169
Keywords:
R301-01/02/03
900.00
Article number:
R212-01/02
HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper) is a special kit for cDNA one-strand synthesis that contains a genomic DNA removal step. The HiScript® II Reverse Transcriptase based on the Kit has a significant improvement compared with the previous generation HiScript® Reverse Transcriptase. Thermal stability, template affinity and inhibitor tolerance. The gDNA wiper used in the Kit can quickly remove genomic DNA contamination at 42°C for 2 minutes, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across exons. 10 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo dT23VN included in the kit has a stronger anchoring ability for Poly A+ RNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo dT23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can synthesize full-length cDNA for cloning (up to 20 kb), but also synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR   Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 10 pg-5 μg total RNA, can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.; gDNA wiper Mix can quickly and completely remove genome contamination   Store at -20℃
900.00
Article number:
R212-01/02
Number of views:
6435
Keywords:
R212-01/02
105.00
Article number:
R211-01/02
HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation of HiScript® Reverse Transcriptase, HiScript® II has further greatly improved thermal stability and is very suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript® II adds multiple point mutations, which further enhances template affinity and progress, and has higher tolerance to common reverse transcription inhibitors, which greatly improves the ability to synthesize full-length cDNA. HiScript® II 1st Strand cDNA Synthesis Kit is a first-strand cDNA synthesis kit based on HiScript® II Reverse Transcriptase. It contains all the components needed to synthesize high-quality first-strand cDNA. The product is suitable for subsequent PCR, qPCR and other experiments. 2 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo (dT)23VN included in the kit has a stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo (dT)23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can not only synthesize full-length cDNA (up to 20 kb) for cloning, but also efficiently synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR.   Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 1 pg-5 μg total RNA, and can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.   Store at -20℃
105.00
Article number:
R211-01/02
Number of views:
5528
Keywords:
R211-01/02
31.88
Article number:
R201-01/02
HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation HiScript® Reverse Transcriptase, HiScript® II Transcriptase has further greatly improved thermal stability, with a half-life of more than 4 hours at 50°C, and can be reacted for a long time at 55°C to maintain stability. It is very suitable for complex secondary Reverse transcription of structural RNA template. In addition, HiScript® II Transcriptase adds a number of point mutations, which further enhances template affinity and progression, greatly improving the synthesis ability of full-length cDNA, and obtaining cDNA up to 20 kb. It has a higher tolerance to common reverse transcription inhibitors and is very suitable for the reverse transcription reaction of plant tissue RNA rich in polysaccharides and polyphenols.   The half-life at 50℃ exceeds 240 min, and the half-life at 55℃ can reach 60 min; CDNA up to 10 kb can be synthesized in 5 minutes, which is suitable for rapid reverse transcription reaction; Higher impurity tolerance and higher detection sensitivity, reverse transcriptase is the first choice for molecular diagnosis   Store at -20℃
31.88
Article number:
R201-01/02
Number of views:
4621
Keywords:
R201-01/02
9999.00
Article number:
R111-01/02
HiScript® Reverse Transcriptase is a brand-new reverse transcriptase obtained through multiple mutations on the basis of M-MLV (RNase H-) Reverse Transcriptase. HiScript® Reverse Transcriptase has the highest activity at 50°C and can withstand reaction temperatures as high as 55°C. It can be used for reverse transcription of RNA templates with secondary structure. By enhancing the affinity with the template, more full-length cDNA can be obtained, and the total RNA can be detected as low as 1 pg. It is especially suitable for reverse transcription of a small number of templates and low-copy genes. In addition, the mismatch rate of HiScript® Reverse Transcriptase is also lower than that of M-MLV (RNase H-) Reverse Transcriptase, which improves the fidelity of cloning. The HiScript® First-strand cDNA Synthesis Kit is based on HiScript® Reverse Transcriptase and contains all the ingredients needed to synthesize high-quality first-strand cDNA, suitable for subsequent PCR, qPCR and PCR cloning. 2 × RT Mix contains optimized buffer system and dNTP; Enzyme Mix contains HiScript®Reverse Transcriptase and RNase inhibitor. Oligo (dT)18, Random hexamers, or gene-specific primers can be selected as reverse transcription primers according to needs.    HiScript ® Reverse Transcriptase provides reliable reverse transcription performance; A wide range of template amount, which can be successfully reverse transcribed from 10 pg-5 μg total RNA; Efficient synthesis ability: can synthesize up to 10 kb cDNA; Different primers can be selected for different downstream applications. The synthesized one-strand cDNA can be widely used in molecular cloning, hybridization, PCR amplification, and qPCR reactions.    Store at -20℃
9999.00
Article number:
R111-01/02
Number of views:
3922
Keywords:
R111-01/02
9999.00
Article number:
R101-01/02
HiScript® Reverse Transcriptase is a new reverse transcriptase based on M-MLV (RNase H-) Reverse Transcriptase through multiple mutations. HiScript® Reverse Transcriptase has the highest activity at 50°C and can withstand reaction temperatures up to 55°C, and can be used for reverse transcription of RNA templates with secondary structure. By enhancing the affinity with the template, more full-length cDNA can be obtained, and the total RNA can be detected as low as 1 pg. It is especially suitable for reverse transcription of a small number of templates and low-copy genes. In addition, the mismatch rate of HiScript® Reverse Transcriptase is also lower than that of M-MLV (RNase H-) Reverse Transcriptase, which improves the fidelity of cloning.    Tolerate a reaction temperature of 50℃, suitable for RNA templates with secondary structure; The template has strong affinity, and more full-length cDNA can be obtained; Highly sensitive, can detect as little as 10 pg of total RNA   Store at -20℃
9999.00
Article number:
R101-01/02
Number of views:
3179
Keywords:
R101-01/02
47.00
Article number:
R021-01
Wild-type M-MLV (Moloney Murine Leukemia Virus) has the following activities: RNA-dependent DNA polymerase activity; DNA-dependent DNA polymerase activity; RNase H activity. Because RNase H activity can catalyze the degradation of RNA in the DNA/RNA hybrid strand, the template RNA may be degraded in the cDNA one-strand synthesis reaction. M-MLV (H-) Reverse Transcriptase is an M-MLV mutant with loss of RNase H activity obtained by site-directed mutagenesis. Compared with the common mutants obtained by deleting the RNase H domain, this product retains the complete protein structure, so it has the same polymerase activity as the wild type, and can be used for longer cDNA synthesis and full-length cDNA library Construction, etc. It also has strand displacement activity and can be used in experiments such as 5'RACE.   Point mutations eliminate RNase H activity to obtain long cDNA products; Stable and reliable reverse transcription performance for RNA templates above 100 ng; Synthetic fragment ≤5 kb; Can be used for experiments such as 5’-RACE reaction and cDNA library construction   Store at -20℃
47.00
Article number:
R021-01
Number of views:
3282
Keywords:
R021-01

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