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NGS Library Prep kits

Molecular Research

Drug Discovery

Competent Cell

9999.00
Article number:
C505-02/03
Fast-T1 is currently the fastest growing competent cell. Outer membrane receptor mutation (tonA) confers resistance to lytic phage T1 and T5 in competent cells; deletion of endonuclease (endA) increases the yield and quality of plasmid DNA; recombinase deficiency (recA) reduces inserts The probability of homologous recombination ensures the stability of inserted DNA; lacZΔM15 makes Fast-T1 competent cells available for blue-white screening. The company’s Fast-T1 competent cells are prepared by a special process, and the pUC19 plasmid is used to detect the transformation efficiency ≥108 cfu/μg DNA.   Fast growth: clones can be seen after 8-9 hours of culture on ampicillin-resistant plates; single clones cultured overnight in 2 ml of LB liquid medium can be cultured for 4-5 hours to extract a small amount of plasmid DNA; Compatible resistance: T1 and T5 phage resistance; Simple operation: for ampicillin-resistant plasmids and ligation products, rapid transformation can be completed in 5 minutes   Store at -70°C (do not store in -20°C or liquid nitrogen!)
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Article number:
C505-02/03
Number of views:
3843
Keywords:
C505-02/03
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Article number:
C504-02/03
This product is a chemically transformed super competent cell prepared using E. coli BL21 (DE3) strain, with a transformation efficiency of over 107 cfu/μg. Suitable for expression vectors of T7 promoter system (such as pET series), genotype F-ompThsdS(rB-mB-) galdcm (DE3).   The genetic background is clear; BL21 (DE3) strain, a high-efficiency protein expression host with T7 RNA polymerase as the expression system; Suitable for non-toxic protein recombinant expression   Store at -80°C (do not store in -20°C or liquid nitrogen!)
9999.00
Article number:
C504-02/03
Number of views:
3484
Keywords:
C504-02/03
9999.00
Article number:
C503-02/03
This product is prepared from chemically competent cells for cloning using a modified Inoue method, which enhances the transformation efficiency of large fragments of DNA and greatly increases the growth rate of host bacterial cells. The transformation efficiency exceeds 108 cfu/μg, which is widely used in plasmid transformation, gene cloning, blue-white spot screening and other purposes. Improved Escherichia coli enhances the transformation efficiency of large fragments of DNA and greatly increases the growth rate of host bacteria cells. Genotype Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lacTetr Hte [F′ proAB lacI qZΔM15 Tn10(Tet r) Amy Camr]   XL10 strain, resistant to tetracycline (Tetr) and chloramphenicol (Camr), a new cloned strain; Suitable for efficient conversion of PCR products, cDNA and other sources of unmethylated DNA; EndA1, RecA1 mutations ensure the stability of foreign DNA in the host bacteria and facilitate the extraction of high-quality plasmid DNA; Hte genotype, enhances the transformation efficiency of large fragments of DNA, and greatly increases the growth rate of host bacterial cells; No foreign plasmid DNA residue, transformation efficiency ≥108 cfu/μg pUC19   Store at -80°C (do not store in -20°C or liquid nitrogen!)
9999.00
Article number:
C503-02/03
Number of views:
2640
Keywords:
C503-02/03
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Article number:
C502-02/03
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Article number:
C502-02/03
Number of views:
6212
Keywords:
C502-02/03

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