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NGS Library Prep kits

Molecular Research

Drug Discovery

Traditional / TA Cloning

143.00
Article number:
C311
5minTM Universal Ligation Mix is ​​a ready-to-use 2× premix that mixes enzyme and Buffer. It contains T4 DNA ligase, which catalyzes the formation of adjacent 5´-phosphate ends and 3´-hydroxyl ends on double-stranded DNA or RNA to form phosphate diphosphates. Ester bond. This kit is not only suitable for sticky end ligation, but also compatible with DNA ligation with blunt ends and single-base overhanging ends. The optimized connection enhancer and reaction buffer make the reaction more efficient and convenient. The reaction can be quickly connected at 25°C for 5 minutes. The ligation product can directly transform a variety of chemically competent cells.   Compatible: compatible with sticky ends, smooth ends, TA connection, Linker or Adapter connection; Fast: 25℃, 5 minutes can be connected quickly; High efficiency: High cloning efficiency.    Store at -30 ~ -15°C
143.00
Article number:
C311
Number of views:
3859
Keywords:
5min
and
ends
the
ligation
compatible
dna
connection
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reaction
17.40
Article number:
C301-01
T4 DNA Ligase can catalyze the formation of phosphodiester bonds between the 5'phosphate end and 3'hydroxyl end of the adjacent nucleic acid at the blunt or sticky end of dsDNA. It can also catalyze the connection between RNA and ssDNA or RNA strands in the double strand, but it cannot catalyze the entire single Chain nucleotide connection. Suitable for nucleic acid operations such as labeling RNA 3'-end, circularizing RNA and DNA oligonucleotides and cloning cDNA.   Purity is greater than 99%, no exogenous nuclease activity remains; The classic buffer composition allows the ligation reaction to proceed efficiently   Store at- 20°C
17.40
Article number:
C301-01
Number of views:
4596
Keywords:
C301-01
C301

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