This kit uses a DNA polymerase with extremely high fidelity and an optimized reaction system to quickly amplify plasmids from bacterial liquid or colonies to obtain DNA that can be used for sequencing reactions. Using this kit to prepare plasmid DNA for sequencing reactions can save a lot of shaking time and cost, simplify the sample preparation process, and accelerate the speed from sample receipt to result output. Under normal circumstances, each reaction can produce 1.5-2.5 µg of plasmid DNA. The product DNA can be used directly in the sequencing reaction.
Rapid amplification of plasmids, directly used in sequencing reactions;
Save shaking time and cost, simplify sample preparation process
Store at -20℃, except for Enzyme Mix v2, the rest can be stored at 4℃ for 2 weeks after defrosting
This product is suitable for direct amplification of plant leaves, seeds, etc., and can be used for high-throughput screening of non-polysaccharide and polyphenol plant samples. Direct-expanded DNA polymerase modified by directed evolution has strong tolerance to PCR inhibitors in plants, while maintaining high amplification performance, suitable for the amplification of DNA fragments within 5 kb; in the kit The unique lysis buffer A can be used to lyse fresh or frozen plant tissues. The operation is simple, and the resulting lysate can be used as an amplification template without purification. The protective agent added to the lysate allows the crude product to be effectively amplified after multiple freeze-thaw cycles. The pre-configured 2 × Plant Direct Master Mix only needs to add primers and templates to perform amplification reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. The amplified products are blunt-ended and are suitable for ClonExpress® and TOPOTM rapid cloning kits (C112/C113/C115/C601).
Unique super-resistant DNA polymerase: Direct-expanded DNA polymerase modified by directed evolution has strong tolerance to PCR inhibitors in plants, while maintaining extremely high amplification performance;
Broad sample compatibility: suitable for the amplification of various common plant leaves and crude seeds;
Very high stability: 50 times of repeated freezing and thawing, the activity has not been significantly reduced;
Convenient operation: Without complicated DNA extraction steps, PCR can be performed using a few leaves or simple lysis, saving a lot of time.
2× Plant Direct Master Mix can be stored at -20℃; Plant Direct Lysis Buffer can be stored at -20℃ or 2-8℃
This kit contains a complete set of crude DNA extraction from animal tissues and QPCR amplification system with anti-pollution probe method, which is suitable for rapid genotyping of mice (Rapid Genotyping). This kit can be used to quickly release genomic DNA from mouse tails, ears, toes and other tissues. The products are directly amplified by QPCR, avoiding operations such as multiple open capping and pipetting. The genotype results are directly obtained by software analysis, avoiding manual checking The input introduces errors and can greatly reduce the time-consuming experiment. The dUTP/UDG anti-pollution system in the reagent can rapidly degrade U-containing pollutants at room temperature, significantly reducing aerosol pollution and improving the detection throughput and reproducibility of results.
The animal tissue can be processed quickly;
The lysate after briefly heating is directly used as a template for quantitative PCR detection based on the dual-probe method based on heat-sensitive UDG anti-contamination, and the corresponding genotype can be directly output by the instrument software to avoid manual verification of data to introduce errors;
The operation is simple and convenient, effectively preventing pipetting contamination and DNA aerosol contamination.
1 x Mouse tissue Lysis Buffer is stored at 4°C; the remaining components are stored at -20°C; please avoid repeated freezing and thawing during use. If the amount is small each time, it is recommended to use small portions.
Blood direct PCR Kit can directly perform PCR on whole blood samples without DNA purification or sample pretreatment. The types of blood samples that can be used for direct amplification include: fresh blood, blood stored at 4°C, and frozen blood
Liquid and dried blood stains stored on Whatman903® and FTA® commercial cards, and compatible with all conventional anticoagulants (EDTA, citrate, heparin, etc.). The kit contains Phanta Blood Super-Fidelity DNA Polymerase, which is engineered on the basis of Phanta Super-Fidelity DNA Polymerase. The enzyme is highly resistant to PCR inhibitors in whole blood samples, and can amplify whole blood concentrations up to 40%. Coupled with carefully optimized 2 × Phanta Blood Buffer V2, Blood Direct PCR Kit V2 can efficiently amplify genome fragments up to 10 kb from whole blood samples. Phanta Blood Super-Fidelity DNA Polymerase amplification products are blunt-ended, suitable for ClonExpress® rapid cloning kit (C112/C113). The kit is equipped with a Positive control primer mix compatible with mammals and many other vertebrates, which can be used for positive control reactions.
Based on the modified Phanta® Blood Super-Fidelity DNA Polymerase, it has extremely strong amplification efficiency and PCR inhibitor tolerance;
Suitable for various blood samples, compatible with conventional anticoagulants;
Can tolerate up to 40% of whole blood template;
Can efficiently amplify genome fragments up to 7.5 kb from whole blood
Store at -20°C
This kit contains a complete set of crude DNA extraction and PCR amplification systems, suitable for rapid genotyping of mice (Rapid Genotyping). This kit can be used to quickly release genomic DNA from mouse tails, ears, and toes. PCR amplification can be performed directly, without homogenization, disruption, overnight digestion, phenol-chloroform extraction, DNA precipitation or column purification, etc., which greatly reduces the time-consuming experiment. When using, soak the tissue in pre-added Proteinase K In the lysate, incubate at 55°C for 20 minutes and heat at 95°C for 5 minutes to inactivate Proteinase K. The lysate can be directly used as a PCR amplification template after centrifugation, and it is widely applicable to the amplification of target fragments within 2 kb after repeated testing. Suitable for multiple PCR reactions within 4 pairs of primers.
The kit is equipped with 2 × Taq Plus Master Mix (Dye Plus), including high-performance Taq Plus DNA Polymerase, dNTP and optimized buffer system. The PCR reaction only needs to add primers and templates to perform amplification, which reduces operations such as tube opening/pipetting, significantly reduces sample cross-contamination and improves detection throughput and results reproducibility. The unique protective agent allows Taq Plus Master Mix to maintain stable activity after repeated freezing and thawing. The system is premixed with electrophoresis buffer and dyes, and electrophoresis can be carried out directly after the reaction, which is convenient and quick to use. The PCR product has A at the 3'end, which can be cloned into the T vector and is suitable for ClonExpress rapid cloning kit (Vazyme #C112/C113/C114).
Special lysate based on proteinase K;
The lysate is directly used for PCR amplification without the need to extract the genome;
It is widely suitable for the amplification of target fragments within 2 kb, and is suitable for multiple PCR reactions within 4 pairs of primers.
1 × Mouse tissue Lysis Buffer is stored at 4°C; the remaining components are stored at -20°C.
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