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NGS Library Prep kits

Molecular Research

Drug Discovery

DNA Methylation

57.00
Article number:
EM202-01/02/03
This product contains EpiArtTM HS Taq DNA Polymerase, dNTP and optimized buffer system, which is suitable for the PCR reaction of bisulfite conversion processing DNA. Compared with ordinary DNA, the DNA damage after bisulfite conversion is serious, which will affect the PCR reaction performance. This product uses a hot-start EpiArtTM HS Taq DNA Polymerase modified by enzyme engineering and an optimized buffer system. Compared with ordinary PCR reagents, this product has better amplification efficiency, sensitivity and specificity for the bisulfite-converted DNA . The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification when used in PCR reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. EM202 contains blue dye, which can be directly used for electrophoresis after the reaction, which is convenient to use. This product can be used with EpiArtTM DNA Methylation Bisulfite Kit (Vazyme #EM101). The PCR product has A at the 3'end, which can be directly cloned into the T vector. It is suitable for ClonExpress® rapid cloning kit (Vazyme #C112/C113/C115) ) And 5 min TA/Blunt-Zero Cloning Kit (Vazyme #C601).   (1) Optimized buffer system, suitable for PCR reaction of bisulfite conversion processing DNA (2) Compared with ordinary PCR reagents, it has better amplification efficiency, sensitivity and specificity for DNA after bisulfite conversion (3) EM202 contains blue dye, which can be directly electrophoresed after the reaction, which is convenient to use   Store at -30 ~ -15℃, transport at -20 ~ 0℃    
57.00
Article number:
EM202-01/02/03
Number of views:
1684
Keywords:
epiart
the
dna
which
and
pcr
for
is
bisulfite
product
57.00
Article number:
EM201-01/02/03
This product contains EpiArtTM HS Taq DNA Polymerase, dNTP and optimized buffer system, which is suitable for the PCR reaction of bisulfite conversion processing DNA. Compared with ordinary DNA, the DNA damage after bisulfite conversion is serious, which will affect the PCR reaction performance. This product uses a hot-start EpiArtTM HS Taq DNA Polymerase modified by enzyme engineering and an optimized buffer system. Compared with ordinary PCR reagents, this product has better amplification efficiency, sensitivity and specificity for the bisulfite-converted DNA . The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification when used in PCR reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. EM202 contains blue dye, which can be directly used for electrophoresis after the reaction, which is convenient to use. This product can be used with EpiArtTM DNA Methylation Bisulfite Kit (Vazyme #EM101). The PCR product has A at the 3'end, which can be directly cloned into the T vector. It is suitable for ClonExpress® rapid cloning kit (Vazyme #C112/C113/C115) ) And 5 min TA/Blunt-Zero Cloning Kit (Vazyme #C601).   (1) Optimized buffer system, suitable for PCR reaction of bisulfite conversion processing DNA (2) Compared with ordinary PCR reagents, it has better amplification efficiency, sensitivity and specificity for DNA after bisulfite conversion (3) EM202 contains blue dye, which can be directly electrophoresed after the reaction, which is convenient to use    Store at -30 ~ -15℃, transport at -20 ~ 0℃
57.00
Article number:
EM201-01/02/03
Number of views:
1510
Keywords:
epiart
the
dna
which
and
pcr
for
is
bisulfite
product
177.00
Article number:
EM101-01/02
DNA methylation is closely related to gene expression and gene function. It plays an important role in physiological and pathological processes such as genetic imprinting, embryonic development, chromosome gene silencing, and cell cycle regulation. Efficient and accurate detection of DNA methylation is important for biological Research in many aspects such as science, genetics, pathology, pharmacology, medical diagnosis, etc. has become more and more important. The most commonly used method to detect DNA methylation is the bisulfite conversion method. The principle of this technology is to use bisulfite Salt treatment of DNA converts unmethylated cytosine into uracil, while methylated cytosine remains unchanged during the conversion process. After conversion, the methylation of DNA can be detected by PCR amplification or DNA sequencing determination. This kit uses thermal denaturation to replace the previous chemical denaturation, combining DNA denaturation and bisulfite conversion into one step, and the conversion reaction time only takes 140 minutes; using membrane desulphonation technology to expand the DNA input range to 100 pg-2 μg; recovery efficiency ≥ 80%, conversion efficiency of unmethylated cytosine ≥ 99%. The transformed product is suitable for downstream applications such as PCR amplification and NGS sequencing.   (1) Optimized buffer system, suitable for PCR reaction of bisulfite conversion processing DNA (2) Compared with ordinary PCR reagents, it has better amplification efficiency, sensitivity and specificity for DNA after bisulfite conversion (3) EM202 contains blue dye, which can be directly electrophoresed after the reaction, which is convenient to use   Store at 15-20℃. Transport at room temperature. The dissolved CT Conversion Mix can be stored at room temperature (15 ~ 25℃) for 24 hours, and frozen (-30 ~ -15℃) for 1 month; Note that it needs to be stored away from light.  
177.00
Article number:
EM101-01/02
Number of views:
2489
Keywords:
epiart
the
dna
and
conversion
to
is
for
of
bisulfite

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