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NGS Library Prep kits

Molecular Research

Drug Discovery

CUT&Tag

1124.00
Article number:
TD902-01/02
HyperactiveTM In-Situ ChIP Library Prep Kit for Illumina is a special kit developed for CUT&Tag technology. CUT&Tag technology is a new method for studying protein-DNA interaction. By fusing Protein G or Protein A with the engineered ultra-highly active Tn5 transposase, a new fusion enzyme (Hyperactive pG- Tn5/pA-Tn5 Transposase), under the guidance of the antibody, accurately target and cut the DNA sequence near the target protein. Compared with traditional ChIP-Seq, this technology has the advantages of low cell input, short experiment cycle, high signal-to-noise ratio, and good repeatability. It is especially suitable for early embryonic development, stem cells, tumors, and epigenetics. field. All reagents in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.   Simple operation: using enzyme digestion instead of ultrasonic disruption, it only takes 1 day to realize the transformation from cells to next-generation sequencing libraries High activity: DNA fragmentation activity is extremely high Low starting cell volume: compatible with as few as 50 cells High purity: high protein purity, low nucleic acid residue     BOX 1: ConA beads are stored at 2 ~ 8℃, and the remaining components are stored at room temperature. BOX 2: 5% Digitonin stored at -30 ~ -15℃, and can be stored at room temperature for 1 week; 10 × Binding Buffer -30 ~ -15 ℃ storage, 2 ~ 8 ℃ can be stored for 6 months; Store the remaining components at -30 ~ -15°C.  
1124.00
Article number:
TD902-01/02
Number of views:
2680
Keywords:
illumina
the
and
for
at
stored
high
is
of
protein
1124.00
Article number:
TD901-01/02
HyperactiveTM In-Situ ChIP Library Prep Kit for Illumina is a dedicated kit developed for CUT&Tag technology. CUT&Tag technology is a new method for studying protein-DNA interactions. By fusing Protein G or Protein A with the engineered ultra-highly active Tn5 transposase, a new fusion enzyme with dual activity (Hyperactive pG- Tn5/pA-Tn5 Transposase), under the guidance of the antibody, accurately target and cut the DNA sequence near the target protein. Compared with traditional ChIP-Seq, this technology has the advantages of low cell input, short experiment cycle, high signal-to-noise ratio, and good repeatability. It is especially suitable for early embryonic development, stem cells, tumors, and epigenetics. field. All reagents in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.   Simple operation: DNA fragmentation and adaptor ligation can be realized in one step, and the transformation from cell to next-generation sequencing library can be realized in only 9 hours Super high activity: both Protein A&Tn5 activity, extremely high DNA fragmentation activity Low initial cell volume: compatible with as few as 60 cells, even single cells High purity: high protein purity, low nucleic acid residue   BOX 1: ConA beads are stored at 2 ~ 8℃, and the remaining components are stored at room temperature. BOX 2: 5% Digitonin stored at -30 ~ -15℃, and can be stored at room temperature for 1 week; 10 × Binding Buffer -30 ~ -15 ℃ storage, 2 ~ 8 ℃ can be stored for 6 months; Store the remaining components at -30 ~ -15°C.  
1124.00
Article number:
TD901-01/02
Number of views:
3812
Keywords:
illumina
the
and
for
at
stored
high
protein
be
activity
9999.00
Article number:
S701-01/02
 Hyperactive pA-MNase for CUT&RUN is the fusion of Protein A and engineered ultra-highly active MNase to form a new fusion enzyme with both MNase exonuclease activity and Protein A activity, which is specifically suitable for protein-genome interaction research CUT&RUN technology. Compared with the traditional protein-genome interaction research method ChIP-Seq, CUT&RUN has significant advantages. This technology has a short experimental cycle, high signal-to-noise ratio, good repeatability, and low cell input. It is especially suitable for early embryo development, Research fields such as stem cells, tumors and epigenetics.   (1) Simple and fast workflow: DNA denaturation and bisulfite conversion are completed in one step, and the conversion reaction time is only 140 min (2) High yield: 10 pg-100 ng genomic DNA starting amount, recovery efficiency ≥ 80%, conversion efficiency of unmethylated cytosine ≥ 99% (3) Suitable for various downstream applications: Bisulfite-converted DNA can be used for stable PCR amplification for downstream analysis of NGS sequencing, etc. (4) Compatible with DNA templates from different sources: DNA extracted from cells or tissues of animals, plants, microorganisms; cfDNA; purified PCR products, etc.   Store at -30 ~ -15℃, and transport at -20 ~ 0℃.    
9999.00
Article number:
S701-01/02
Number of views:
1895
Keywords:
cut&run
and
for
dna
is
of
the
research
conversion
958.00
Article number:
S602-01/02
 Hyperactive pG-Tn5 Transposase for CUT&Tag is a fusion of Protein G and engineered ultra-highly active Tn5 transposase to form a new fusion enzyme with both transposase and Protein G activities. It is specially suitable for protein-genome interactions. Researched CUT&Tag technology. Compared with the traditional protein-genome interaction research method ChIP-Seq, CUT&Tag has significant advantages. This technology has a short experimental period, high signal-to-noise ratio, good repeatability, and low cell input. It is especially suitable for early embryo development, Research fields such as stem cells, tumors and epigenetics.   Simple operation: DNA fragmentation and adaptor ligation can be realized in one step, and the transformation from cell to next-generation sequencing library can be realized in only 9 hours Super high activity: both Protein A&Tn5 activity, extremely high DNA fragmentation activity Low initial cell volume: compatible with as few as 60 cells, even single cells High purity: high protein purity, low nucleic acid residue   The entire kit is stored at -85 ~ -65°C and shipped on dry ice; Hyperactive pG-Tn5 Transposase can be stored at -85 ~ -65°C after receipt, and the remaining components can be stored at -30 ~ -15°C; the resulting transposition The seeds are stored at -30 ~ -15℃, and they are valid for one year.    
958.00
Article number:
S602-01/02
Number of views:
3361
Keywords:
cut&tag
and
the
high
protein
is
at
be
stored
transposase
958.00
Article number:
S603-01/02
958.00
Article number:
S603-01/02
Number of views:
3361
Keywords:

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