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NGS Library Prep kits

Molecular Research

Drug Discovery

CUT&Tag

9999.00
Article number:
S701-01/02
 Hyperactive pA-MNase for CUT&RUN is the fusion of Protein A and engineered ultra-highly active MNase to form a new fusion enzyme with both MNase exonuclease activity and Protein A activity, which is specifically suitable for protein-genome interaction research CUT&RUN technology. Compared with the traditional protein-genome interaction research method ChIP-Seq, CUT&RUN has significant advantages. This technology has a short experimental cycle, high signal-to-noise ratio, good repeatability, and low cell input. It is especially suitable for early embryo development, Research fields such as stem cells, tumors and epigenetics.   (1) Simple and fast workflow: DNA denaturation and bisulfite conversion are completed in one step, and the conversion reaction time is only 140 min (2) High yield: 10 pg-100 ng genomic DNA starting amount, recovery efficiency ≥ 80%, conversion efficiency of unmethylated cytosine ≥ 99% (3) Suitable for various downstream applications: Bisulfite-converted DNA can be used for stable PCR amplification for downstream analysis of NGS sequencing, etc. (4) Compatible with DNA templates from different sources: DNA extracted from cells or tissues of animals, plants, microorganisms; cfDNA; purified PCR products, etc.   Store at -30 ~ -15℃, and transport at -20 ~ 0℃.    
9999.00
Article number:
S701-01/02
Number of views:
1895
Keywords:
cut&run
and
for
dna
is
of
the
research
conversion
958.00
Article number:
S602-01/02
 Hyperactive pG-Tn5 Transposase for CUT&Tag is a fusion of Protein G and engineered ultra-highly active Tn5 transposase to form a new fusion enzyme with both transposase and Protein G activities. It is specially suitable for protein-genome interactions. Researched CUT&Tag technology. Compared with the traditional protein-genome interaction research method ChIP-Seq, CUT&Tag has significant advantages. This technology has a short experimental period, high signal-to-noise ratio, good repeatability, and low cell input. It is especially suitable for early embryo development, Research fields such as stem cells, tumors and epigenetics.   Simple operation: DNA fragmentation and adaptor ligation can be realized in one step, and the transformation from cell to next-generation sequencing library can be realized in only 9 hours Super high activity: both Protein A&Tn5 activity, extremely high DNA fragmentation activity Low initial cell volume: compatible with as few as 60 cells, even single cells High purity: high protein purity, low nucleic acid residue   The entire kit is stored at -85 ~ -65°C and shipped on dry ice; Hyperactive pG-Tn5 Transposase can be stored at -85 ~ -65°C after receipt, and the remaining components can be stored at -30 ~ -15°C; the resulting transposition The seeds are stored at -30 ~ -15℃, and they are valid for one year.    
958.00
Article number:
S602-01/02
Number of views:
3361
Keywords:
cut&tag
and
the
high
protein
is
at
be
stored
transposase
958.00
Article number:
S603-01/02
958.00
Article number:
S603-01/02
Number of views:
3361
Keywords:

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