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NGS Library Prep kits

Molecular Research

Drug Discovery

WTA

440.70
Article number:
N712-01/02/03
Single Cell Full Length mRNA Amplication Kit can use 1-500 cells or 10 pg-10 ng total RNA as template, Oligo(dT) VN Primer as reverse transcription primer for cDNA synthesis, and use Sc Reverse Transcriptase reverse transcriptase Template-switching activity adds a linker sequence to the 3'end of the cDNA, and then performs subsequent PCR amplification through the linker sequence to obtain a full-length cDNA amplification product, effectively avoiding 3'preference and rRNA contamination during cDNA synthesis. The obtained full-length cDNA amplification products can be analyzed for genetic regulation information such as gene expression differences, alternative splicing, and fusion genes. The Single Cell Full Length mRNA Amplication Kit is compatible with sample volumes within 2.5 μl. Generally, 2-20 ng cDNA amplification products can be obtained in one reaction.   Low template starting volume; High amplification sensitivity; Good product integrity   Box 1, -70°C storage; Box 2, store at -20°C. 
440.70
Article number:
N712-01/02/03
Number of views:
3408
Keywords:
single
cdna
amplification
the
and
as
reverse
can
mrna
amplication
9999.00
Article number:
N711-01/02/03
Discover-scTM WTA Kit V2 can use 1-1000 cells or 10 pg-10 ng total RNA as a template. Through the synthesis and amplification of the first-strand cDNA, enough samples for sequence analysis can be obtained, thereby solving problems such as single Because of the low RNA content of cells and other small samples, it is a technical problem that conventional mRNA-seq cannot be used for sequencing analysis. The kit uses Oligo dT Primer as the reverse transcription primer for cDNA synthesis, and uses the Template-switching activity of Discover-sc Reverse Transcriptase to add a linker sequence to the 3'end of the cDNA, and then performs subsequent PCR amplification through the linker sequence to obtain the complete Long cDNA amplification product effectively avoids 3'preference and rRNA contamination during cDNA synthesis. Discover-scTM WTA Kit V2 is an upgraded version developed on the basis of Discover-scTM WTA Kit. Its detection sensitivity and volume compatibility have been greatly improved, making it more suitable for the detection of low-abundance genes and low-concentration templates. In general, one reaction can produce 2-20 ng cDNA amplification products.    Low template starting volume; High amplification sensitivity; Good product integrity; Operation success rate is high; Wide volume compatibility   Box 1, -70°C storage; Box 2, store at -20°C. 
9999.00
Article number:
N711-01/02/03
Number of views:
3141
Keywords:
wta
the
and
of
cdna
amplification
for
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as

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