Discover-sc® Single Cell WGA Kit uses an isothermal amplification system based on Multiple Strand Displacement Amplification (MDA) and enables Phi29 DNA polymerase modified by directed evolution, which has higher amplification efficiency and amplification uniformity, and can be used in Achieve undifferentiated whole genome amplification of 1-1000 cells or small samples within 2 hours. The single-cell genome can reach more than 95% coverage after N603 amplification. The size of the amplified product is between 2-100 kb, and the average product length is greater than 20 kb. It is widely used for preimplantation chromosome detection (PGS/PGD) , Tumor cells, stem cells, metagenomics and other research fields. All enzymes and buffers in Discover-sc® Single Cell WGA Kit have undergone strict quality control and functional verification. The optimized experimental system maximizes the uniformity of amplification and ensures stability and repeatability.
Low template starting volume;
High amplification sensitivity;
Good product integrity;
Operation success rate is high;
Wide volume compatibility
Store at -30 ~ -15℃. For longer-term storage, please store below -70℃.
Discover-scTM Single Cell Kit uses an isothermal amplification system based on MDA, which can use a single cell or a small sample as a template to achieve indiscriminate amplification of the whole genome. The single cell genome can reach a coverage of more than 95% after amplification. The size of the amplified product is between 2-100 kb, and the average product length is greater than 20 kb. It can be widely used in whole genome and exome sequencing, large fragment copy number variation analysis, microsatellite analysis, qPCR analysis, and gene chip analysis.
The Phi29 DNA polymerase used in this kit is a DNA polymerase cloned from phage. It has strong strand displacement activity and can realize the melting and replication of complex structures of DNA; and Phi29 DNA polymerase has strong strand affinity , A single polymerization reaction can achieve continuous polymerization extension up to 100 kb, and its amplified products are suitable for almost all downstream applications; Phi29 DNA polymerase also has strong 3'-5' exonuclease activity, and its fidelity The degree is 1000 times that of Taq enzyme, which guarantees the high fidelity of DNA synthesis. Under normal circumstances, one reaction of Discover-scTM Single Cell Kit can produce 30-40 μg of genomic DNA with high coverage.
Store at -20℃. For longer-term storage, please store below -70℃.
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