LOGO
Search
Submit
Cancel
这是描述信息
/
/
/
/
RNA Library Prep - for Illumina®

NGS Library Prep kits

Molecular Research

Drug Discovery

RNA Library Prep - for Illumina®

799.00
Article number:
NR605
VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina is specially designed for the preparation of transcriptome libraries for Illumina platform. The kit is universal and suitable for RNA library construction of RNA that have been obtained by Poly(A)-based mRNA enrichment or rRNA depletion. The kit contains two types of cDNA 2nd Strand synthesis buffer, which can be chosen for library construction for non-stranded or stranded RNA-Seq transcriptome analysis. This kit combines 2nd Strand cDNA synthesis, end-repair and dA-Tailing into one step, with no need of purification, which greatly simplifies the process of library construction and shortens the operation time. The optimized reaction system improves the library construction efficiency, is compatible with lower-input RNA, and has uniform coverage for different amounts of input-RNA. Libraries of specific sizes, which can be customized, can be obtained after size selection with magnetic beads. All the enzymes and buffer provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability.
799.00
Article number:
NR605
Number of views:
1000
Keywords:
kit
799.00
Article number:
NR604-01/02
VAHTS® Universal V6 RNA-seq Library Prep Kit for Illumina is a special kit for transcriptome library construction developed for Illumina high-throughput sequencing platform. The kit contains two types of cDNA two-strand synthesis buffers, which can be selected according to needs Generic transcriptome or strand-specific transcription build library. The kit combines two-strand synthesis, end repair, and dA-Tailing into one step. No purification steps are required in the middle, which greatly simplifies the operation process and shortens the time for library construction. The optimized reaction system improves the efficiency of library conversion, is compatible with lower initial input, and has uniform coverage for different initial amounts of RNA. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet the individual needs of different experiments. All enzymes and buffers included in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.     Shorter time-consuming database construction; More efficient linker connection efficiency, higher library conversion rate; Wider template compatibility range, RNA starting amount can be as low as 10ng; Wide species applicability, can be applied to different species such as humans, animals, plants, etc.    Store at -30°C~-15°C
799.00
Article number:
NR604-01/02
Number of views:
4561
Keywords:
vahts
the
library
and
kit
of
for
to
can
as
1884.00
Article number:
NR603-01/02
VAHTSTM Total RNA-seq (H/M/R) Library Prep Kit for Illumina® is a special kit for chain-specific transcriptome library construction targeted for the Illumina high-throughput sequencing platform. This kit is suitable for the amount of initial template The total RNA of human, mouse, and rat is 0.1-1 μg. The difference from conventional transcriptome library is that this kit can combine rRNA (including cytoplasm 28S, 18S, 5S rRNA and mitochondrial 16S, 12S, 5.8S rRNA). ) Is removed from total RNA, leaving mRNA and other non-coding RNAs Used for the analysis of non-coding RNA such as LncRNA; degraded RNA samples can also be used for library construction with this kit. When the second-strand cDNA is synthesized in this kit, dUTP is added to label it, and the labeled second-strand is digested with UDG enzyme before PCR, so the sequencing information comes from the first-strand cDNA, which ensures the strand directionality of RNA Analyze the sequencing data to determine whether the transcript is from the sense or antisense DNA strand. The kit includes three independent packages of NR4, NR5 and NR6, which contain the library construction process All necessary enzymes, buffers, and all components have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent. Adapters and DNA and RNA purification magnetic beads need to be prepared separately. Adapters can be selected from VAHTSTM RNA Adapters (Vazyme#N803, Vazyme #N804 or Vazyme #N809, Vazyme #N810, Vazyme #N811, Vazyme#N812). For details on adapter selection, please refer to Common Problems and Solutions 6 (Page17); DNA purification magnetic Beads can choose VAHTSTM DNA Clean Beads (Vazyme #N411) or Agencourt® AMPure® XP reagent (Beckman #A63880,#A63881, #A63882); RNA purification magnetic beads can choose VAHTSTM RNA Clean Beads (Vazyme #N412) or Agencourt® RNAClean ® XP Beads (Beckman #A63987).    Fast: The minimalist operation process reduces the time for building a database by 2 h Compatibility: Compatible with a variety of RNA enrichment modules, with any combination General: Flexible and free choice of chain-specific or non-chain-specific libraries   All components are stored at -20℃
1884.00
Article number:
NR603-01/02
Number of views:
4715
Keywords:
vahts
the
and
rna
is
for
of
beads
library
vazyme
9999.00
Article number:
NR612-01/02
VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina v2 is a special kit for the construction of strand-specific transcriptome libraries developed for the Illumina high-throughput sequencing platform. This kit is suitable for starting template amount of 0.05-4 μg completeness A good chain-specific transcriptome library construction of total RNA of eukaryotes such as animals, plants, and fungi. Unlike conventional transcriptional library construction, the substrate is incorporated into dUTP during the synthesis of the second strand, and is enriched by PCR Use UDG enzyme for the U-labeled second-strand template before the library Digestion ensures that the final sequencing information comes from the first-strand cDNA, thereby preserving the strand directionality of the mRNA. Later data analysis can determine whether the transcript comes from the sense or antisense strand of DNA. This kit is an upgraded product of VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina. This version greatly simplifies the operation process, improves the library conversion efficiency, is compatible with a lower initial input, and uses magnetic bead sorting. A library of a specific length can be quickly obtained to meet the individual needs of different experiments. All enzymes and buffers included in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.    Box1: Store at 2 ~ 8℃; Box2: Store at -30 ~ -15℃. 
9999.00
Article number:
NR612-01/02
Number of views:
3549
Keywords:
vahts
the
of
library
is
for
and
kit
construction
this
9999.00
Article number:
NR611-01/02
VAHTSTM mRNA-seq V3 Library Prep Kit for Illumina® is a special kit for transcriptome library construction targeted for the Illumina high-throughput sequencing platform. This kit is suitable for activities with a starting template amount of 0.01-4 μg and good integrity. The total RNA of eukaryotes such as plants and fungi. The total RNA sample goes through the steps of mRNA isolation, mRNA fragmentation, double-stranded cDNA synthesis, end repair, adaptor ligation, ligation product purification, fragment size sorting, library amplification and other steps. Obtain a sequencing library suitable for the Illumina platform. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet the individual needs of different experiments. The kit includes three independent packages of NR1, NR21 and NR31 , Contains all the enzymes and buffers required for the library construction process. All components have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent. VAHTSTM mRNA-seq V3 Library Prep Kit for Illumina® is an upgraded version developed on the basis of VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina®, which simplifies the operation process, improves library conversion efficiency, and is compatible with lower initial investment The amount of RNA has uniform coverage for different initial amounts of RNA.    Provide 24 kinds of Index connectors; Strict quality control and functional verification of each batch   NR 1, 2-8°C storage; NR 21, stored at -20°C; NR 31, stored at -20°C
9999.00
Article number:
NR611-01/02
Number of views:
4398
Keywords:
vahts
the
of
library
for
and
kit
illumina
is
prep
9999.00
Article number:
NR602-01/02
VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina® is a special kit for transcriptome library construction targeted for the Illumina® high-throughput sequencing platform. This kit is suitable for constructing a starting template with an input amount of 0.1-4 μg total RNA A chain-specific transcriptome library of eukaryotes such as animals, plants, and fungi. The difference from conventional transcriptome library construction is that the total RNA of the sample is separated, fragmented, and first-strand cDNA is synthesized. During synthesis, dUTP was incorporated to label it, and at the same time, the end repair was completed at this step; followed by the steps of adding A-tailing, ligation adapter, ligation product purification, fragment size sorting, library amplification, etc., before PCR enrichment of the library The U-labeled second-strand template is digested with UDG enzyme. Therefore, the final sequencing information comes from the first-strand cDNA, which preserves the strand directionality of the mRNA. Data analysis after sequencing can determine whether the transcript comes from the sense strand or Antisense DNA strand. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet different needs. The kit includes three independent packages of NR1, NR5 and NR6, which contain all the enzymes and buffers needed for the library construction process. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the library to the greatest extent Stability and repeatability of the construction.    Includes Ribo-OffTM rRNA depletion module, which can remove ribosomal RNA from human, mouse, rat, cow, dog, horse, chicken, monkey, pig, zebrafish and other species; Chain-specific library construction; Optimized large fragment library construction scheme   NR 1, 2-8°C storage; NR 5, stored at -20°C; NR 6, stored at -20°C. 
9999.00
Article number:
NR602-01/02
Number of views:
2865
Keywords:
vahts
the
library
of
and
is
kit
for
construction
at
1020.00
Article number:
NR801-01/02
VAHTSTM Small RNA Library Prep Kit for Illumina® is a special kit for constructing small RNA libraries targeted for the Illumina® high-throughput sequencing platform. This kit is suitable for the total RNA of animals and plants with a template starting amount of 0.1-1 μg, as well as the separation and purification of small RNA. Connect the 3'and 5'ends of small RNA with universal adapters, and then go through the steps of reverse transcription, PCR amplification, and purification by PAGE gel or magnetic beads to finally obtain a sequencing library suitable for the Illumina® platform. The kit contains all the enzymes and buffers required for library construction. All components have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.   RL5 Adaptor, stored at -70°C; Filtration Column, stored at room temperature; other components, stored at -20°C.
1020.00
Article number:
NR801-01/02
Number of views:
3347
Keywords:
vahts
the
and
for
of
small
rna
library
kit
at
9999.00
Article number:
NR601-01/02
VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina® is a special kit for transcriptome library construction targeted for the Illumina® high-throughput sequencing platform. This kit is suitable for animals and plants with an initial template amount of 0.1-4 μg And fungi and other eukaryotic total RNA. The total RNA sample goes through the steps of mRNA isolation, fragmentation, double-stranded cDNA synthesis, end repair, A-tailing, adaptor ligation, ligation product purification, fragment size sorting, library amplification, etc. Finally, a sequencing library suitable for the Illumina® platform is obtained. This kit uses magnetic bead sorting to quickly obtain a library of a specific length, which can meet the individual needs of different experiments. The kit includes three NR1, NR2 and NR3 The independent packaging contains all the enzymes and buffers required for the library construction process. All components have undergone strict quality control and functional verification to ensure the stability and repeatability of the library construction to the greatest extent. Adapters and DNA purification magnetic beads need to be prepared separately. Adapters can be selected from VAHTSTM RNA Adapters (Vazyme #N803, Vazyme #N804 or Vazyme #N809, Vazyme #N810, Vazyme #N811, Vazyme #N812). For details on adapter selection, please refer to Common Problems and Solutions 4 (Page16); DNA purification magnetic Beads can choose VAHTSTM DNA Clean Beads (Vazyme #N411) or Agencourt® AMPure® XP reagent (Beckman #A63880, #A63881, #A63882).    The chain directionality of the mRNA is retained, and the gene structure and gene expression information can be obtained more accurately; It has a high success rate for complex plant templates; The library construction process is the same as Illumina TruSeq Stranded mRNA Sample Prep Kit; Optimized large fragment library construction program; The sequencing data is highly consistent with Illumina reagents   NR 1, 2-8°C storage; NR 2, stored at -20°C; NR 3, stored at -20°C
9999.00
Article number:
NR601-01/02
Number of views:
3127
Keywords:
vahts
the
and
library
for
vazyme
is
kit
of
illumina
250.00
Article number:
N809/N810/N811/N812
VAHTSTM RNA Adapters set3-set6 for Illumina® are dedicated kits for Illumina high-throughput sequencing platform library construction, suitable for Illumina high-throughput sequencing platform multi-sample RNA library construction. The set3 kit (N809) contains a total of 24 different Index adapters from RNA Adapter 96-01 to RNA Adapter 96-24; the set4 kit (N810) contains a total of 24 different indexes from RNA Adapter 96-25 to RNA Adapter 96-48 Adapters; set5 kit (N811) contains 24 different Index adapters from RNA Adapter 96-49 to RNA Adapter96-72; set6 kit (N812) contains 24 different indexes from RNA Adapter 96-73 to RNA Adapter 96-96 Index connector. All adapters provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.   Provide 24 kinds of Index connectors; Strict quality control and functional verification of each batch    Store at -20 °C. Do not store this product in an environment where the temperature is higher than room temperature
250.00
Article number:
N809/N810/N811/N812
Number of views:
2471
Keywords:
vahts
rna
adapter
the
to
adapters
24
kit
of
from
62.50
Article number:
N803/N804
VAHTSTM RNA Adapters set1/set2 for Illumina® is a special kit for Illumina high-throughput sequencing platform library construction, suitable for Illumina high-throughput sequencing platform multi-sample RNA library construction. The set1 kit (N803) contains RNA Adapter Nos. 1-12 with a total of 12 different Index adapters; the set2 kit (N804) contains RNA Adapter Nos. 13-27 with a total of 12 different Index adapters. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent.   Provide 24 kinds of Index connectors; Strict quality control and functional verification of each batch    Store at -20 °C
62.50
Article number:
N803/N804
Number of views:
2829
Keywords:
VAHTS RNA Adapters set1/set2 for Illumina®
N803/N804
145.00
Article number:
N813/N814/N815/N816
VAHTSTM Small RNA Index Primer Kit for Illumina® is a dedicated kit for VAHTSTM Small RNA Library Prep Kit for Illumina® (vazyme#NR801). It contains 1 Universal Primer and 48 different small RNA Index Primer, which can be used for 48 different Preparation of single-ended Index-labeled small RNA library. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library construction to the greatest extent.   All components are stored at -20°C.
145.00
Article number:
N813/N814/N815/N816
Number of views:
2100
Keywords:
1219.00
Article number:
N323/N324
VAHTSTM RNA Multiplex Oligos set 1 for Illumina® is a special kit for Illumina high-throughput sequencing platform library construction. The kit contains VAHTS RNA Adapter-S for Illumina, 8 types of VAHTS i5 PCR Primers and 12 types of VAHTS i7 PCR Primers, combined with VAHTSTM mRNA-seq v3 Library Prep Kit for Illumina (Vazyme #NR611), can be used to construct up to 96 different combinations of double-ended Index tag library construction. At the same time, it can be used in conjunction with VAHTSTM RNA Multiplex Oligos set 2 for Illumina to construct a double-ended Index tag library with up to 384 different combinations. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent   Fast: The minimalist operation process reduces the time for building a database by 2 h Compatibility: Compatible with a variety of RNA enrichment modules, with any combination General: Flexible and free choice of chain-specific or non-chain-specific libraries   All components are stored at -20°C. Do not store this product in an environment where the temperature is higher than room temperature.
1219.00
Article number:
N323/N324
Number of views:
2126
Keywords:
vahts
the
to
for
of
illumina
library
with
rna
and

The products on this website may not be sold in all countries or regions. Please contact Vazyme or your local distributor for further information.

这是描述信息

Add:Nanjing State Economy & Technology Development Zone, Red Maple Technology Industrial Park, building C 1-2 Tel:400-600-9335
Copyright 2015 Vazyme Biotech Co.,Ltd All rights reserved 
苏ICP备12014779号  Power by: www.300.cn nanjing.300.cn