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NGS Library Prep kits

Molecular Research

Drug Discovery

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Champagne Taq DNA Polymerase

AceTaq® DNA Polymerase is a chemically modified Taq DNA Polymerase whose activity is completely blocked at room temperature to prevent non-specific amplification and primer dimers during sample preparation and reaction temperature rise. The activity is released only after heating at 95°C. Compared with antibody-based hot-start Taq enzymes, AceTaq® DNA Polymerase has a more complete and stricter activity closure; compared with existing chemically modified hot-start Taq enzymes, the activation time of AceTaq® DNA Polymerase only takes 5 minutes. Compatible with existing PCR programs. AceTaq® DNA Polymerase combined with an optimized buffer system can minimize non-specific amplification and primer dimers, bring the highest sensitivity, and is very suitable for amplifying low-copy genes from complex templates (genome, cDNA). The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® Rapid Cloning Kit (C112-C113).   With chemical modification, the enzyme activity is completely blocked below 80℃; Heat at 95℃ for 5 minutes to release the activity; Suitable for amplifying low-copy genes from complex templates (genome, cDNA); Provide ready-to-use premix to minimize experimental steps   Store at -20°C
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$
96.0
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96.0
Number of views:
4040
Product serial number
P122-d1/d2/d3
Category
Hot Start
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Champagne Taq Antibody is a monoclonal antibody which can inhibit Taq DNA polymerase activity when bound to it. Due to its unique thermo stability and high affinity to Taq DNA polymerase, Champagne Taq Antibody blocks Taq DNA polymerase activity at temperatures up to 65℃ but releases fully active polymerase after heat treatment at 95℃ for 30 seconds. 
Champagne Taq DNA Polymerase is recombinant Taq DNA polymerase contained Champagne Taq antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 95℃ , thereby providing an automatic “hot start” for Taq DNA polymerase in PCR, which provide increased sensitivity,specificity, and yield, while allowing assembly of reactions at room temperature. The use of this antibody helps reduce PCR optimization requirements, reaction set-up time and effort, handling of reaction components, and contamination risk. No modifications to PCR reactions or protocols are necessary. 
Champagne Taq DNA Polymerase has a 5’→3’ DNA polymerase activity and a 5’→3’ exonuclease activity, and lacks a 3’→5’ proofreading function. The obtained PCR products are compatible with ClonExpress II One Step Cloning Kit (C112-C113), and can be directly used for cloning into T-Vectors as most PCR products amplified with Taq DNA polymerase have one A at the 3'-terminus.

With chemical modification, the enzyme activity is completely blocked below 80℃;
Heat at 95℃ for 5 minutes to release the activity;
Suitable for amplifying low-copy genes from complex templates (genome, cDNA);
Provide ready-to-use premix to minimize experimental steps
 

Store at -20°C

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