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NGS Library Prep kits

Molecular Research

Drug Discovery

Immunoassay

506.50
Article number:
DD2103-01
506.50
Article number:
DD2103-01
Number of views:
1000
Keywords:
430.50
Article number:
DD2102-01/02
Add&ReadTM Human Fc Kit can be used to detect the concentration of Human IgG or hFc-fusion protein (hFc-fusion protien) in the cell supernatant or after purification. There is an antibody in the kit that recognizes the Fc region of Human IgG and marks the fluorescent donor Eu (anti-human IgG-Eu); and Human IgG, labeled with fluorescent receptor A2 (Human IgG-A2). In the solution, anti-human IgG-Eu binds to Human IgG-A2, 320 nm light excites fluorescence donor Eu, Eu donor emits 620 nm light, this 620 nm light excites fluorescent acceptor A2, A2 acceptor It emits 665 nm light, which is the phenomenon of fluorescence resonance energy transfer (FRET). When the human IgG or hFc-fusion protien to be tested is added to the solution, it will compete to bind anti-human IgG-Eu and destroy the FRET phenomenon. The concentration of Human IgG or hFc-fusion protien to be tested is inversely proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG, hFc-fusion protein or bispecific antibodies.   Simple steps Has good compatibility Quantitatively accurate   Storage temperature: -30 ~ -15℃, can be transported at -20 ~ 0℃. Diluent and Detection buffer are frozen and transported, and can be stored at 2 ~ 8℃ after use; Anti-human IgG-Eu and Human IgG-A2: It is recommended to store in -20℃ or -70℃ in aliquots to avoid repeated freezing and thawing; IgG Standard: After adding ddH2O to dissolve, it is recommended to aliquot into 1.5 ml EP tubes and store at -20°C or -70°C to avoid repeated freezing and thawing.  
430.50
Article number:
DD2102-01/02
Number of views:
2032
Keywords:
kit
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human
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be
nm
430.50
Article number:
DD2101-01/02
Add&ReadTM Human IgG Kit can be used to detect the concentration of Human IgG (hlgG) in the cell supernatant or after purification. There are two antibodies that recognize hIgG in the kit, namely: the antibody that recognizes the Fc region, anti-hFc (labeled fluorescent donor) Eu, anti-hFc-Eu); antibody anti-hFab (labeled fluorescent receptor A2, anti-hFab-A2) that recognizes the Fab region. When anti-hFc-Eu and anti-hFab-A2 are close (combination occurs), fluorescence resonance energy transfer (FRET) can occur. Use 320 nm light to excite the fluorescence donor Eu, Eu emits 620 nm light, this 620 nm light excites the fluorescent acceptor A2, and A2 emits 665 nm light. The concentration of Human IgG to be tested is directly proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG.   Simple steps Has good compatibility Quantitatively accurate   Storage temperature: -30 ~ -15℃, can be transported at -20 ~ 0℃. Diluent and Detection buffer are frozen and transported, and can be stored at 2 ~ 8℃ after use; Anti-hFc-Eu and Anti-hFab-A2: It is recommended to store in -20℃ or -70℃ in aliquots to avoid repeated freezing and thawing; IgG Standard: After adding ddH2O to dissolve, it is recommended to aliquot into 1.5 ml EP tubes and store at -20°C or -70°C to avoid repeated freezing and thawing.  
430.50
Article number:
DD2101-01/02
Number of views:
1646
Keywords:
kit
the
to
and
nm
be
can
igg
in
at

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