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NGS Library Prep kits

Molecular Research

Drug Discovery

Cell Biology

98.00
Article number:
E211-01
Bradford method (Coomassie Brilliant Blue Method) is one of the most sensitive methods for determining protein concentration. When Bradford staining solution (Coomassie Brilliant Blue G250) and protein are combined under acidic conditions, the color of the solution changes from brown-black to blue , The maximum absorbance wavelength is changed from 456 nm to 595 nm, and the absorbance value has a good linear relationship with the protein content within a certain concentration range. By measuring the absorbance value and comparing the absorbance value of the standard protein, the protein concentration is calculated to achieve The protein concentration determination is fast, stable and highly sensitive. This reagent has been upgraded through research and development, and has a high tolerance to detergents commonly used in protein extraction. Compared with the conventional Bradford protein concentration determination kit, this kit is compatible with a series of common detergents, and overcomes the shortcomings of Bradford method that is sensitive to detergents. Compared with the BCA method, it is compatible with high-concentration reducing agents. And the detection speed is extremely fast, and it is faster when detecting the protein concentration of detergent samples.    The detection of protein has high sensitivity and easy operation; Good linear relationship; It has strong tolerance to detergents and is compatible with very high concentrations of detergents; Good stability, BSA standard protein is more pure   Bradford Reagent 2℃ ~8℃ storage, BSA Standard -30℃ ~-15℃ storage
98.00
Article number:
E211-01
Number of views:
2111
Keywords:
Detergent Compatible Bradford Protein Quantification Kit
E211-01
40.00
Article number:
E112-01/02
The BCA Protein Quantification Kit is currently one of the most sensitive protein determination methods. Under alkaline conditions, the protein reduces Cu2+ to Cu+, and Cu+ interacts with the unique BCA Reagent A (containing BCA). The reaction produces a sensitive color reaction and forms a purple complex. The water-soluble complex shows strong absorbance at A562 nm, and the absorbance and protein concentration have a good linear relationship in a wide range. The absorbance can be calculated Protein concentration. Therefore, use a microplate reader to measure its absorbance at A562 nm and compare it with the standard curve to calculate the concentration of the protein to be tested. Commonly used detergents such as SDS, Triton X-100, Tween-20 do not affect the test results, but are affected by chelating agents (EDTA, EGTA), reducing agents (DTT, mercaptoethanol) and lipids. In the experiment, if the background value of the sample dilution or lysate itself is found to be high, the Bradford protein concentration determination kit can be used.    Simple operation; high sensitivity; wide compatibility; standard curve with high linear relationship   BCA Reagent A/B 2-8°C storage, BSA Standard -30-15°C storage
40.00
Article number:
E112-01/02
Number of views:
3937
Keywords:
29.00
Article number:
E411-03/04/05
29.00
Article number:
E411-03/04/05
Number of views:
2535
Keywords:
E411-03/04/05
198.00
Article number:
DL101-01
The Dual Luciferase Reporter Assay Kit is used to detect the fluorescence intensity of the Luciferin substrate after the reporter plasmid is transfected into the cells, so as to reflect the expression of luciferase and achieve the purpose of detecting gene regulation. First, use luciferin as a substrate to detect Firefly luciferase, and then use coelenterazine as a substrate to detect Renilla luciferase (Renilla luciferase) to achieve dual luciferase reporter genes Detection. The dual luciferase detection kit detects the activity of firefly luciferase to evaluate the effect of regulatory elements induced by stimuli; Renilla luciferase is used as an internal reference for correcting transfection efficiency to eliminate differences in cell number and transfection efficiency between wells To ensure reliable results.   The fluorescent signal is strong; Wide detection range; high sensitivity    Store at -20°C; the dissolved and aliquoted Luciferase Substrate can be stored at -80°C for a long time, or at -20°C for short-term storage for no more than 1 month
198.00
Article number:
DL101-01
Number of views:
4841
Keywords:
DualLuciferaseReporterAssayKit
DL101-01
105.00
Article number:
D103-01/02/03
Myco-OffTM Mycoplasma Cleaner is the latest generation of mycoplasma removal reagent, mainly used to remove mycoplasma in cells, serum and culture media. This product is different from commonly used antibiotics. Its removal principle is to destroy the membrane structure of mycoplasma, causing the mycoplasma to rupture and die; it can effectively remove antibiotic-resistant mycoplasma without causing drug resistance; for common Gram-negative and Positive bacteria also have a certain inhibitory effect. After using this product for 3-7 days, the intracellular and extracellular mycoplasma can be completely removed, and there is no secondary pollution of mycoplasma within 4 months. Myco-OffTM Mycoplasma Cleaner can remove most types of mycoplasma without being toxic to the cell itself; it is suitable for commonly used cell lines such as mouse embryonic stem cells or iPS cells, human embryonic stem cells or iPS cells, HEK293, HeLa, HepG2, HCT116 , COS-7, Vero, Huh-7, MDCK, PANC-1, SW620 and U2OS etc.   Can directly kill mycoplasma instead of inhibiting its growth; it only takes 3-7 days to get a good mycoplasma clearance effect; low cytotoxicity, suitable for a variety of common cell lines and primary cells   Store at -20℃
105.00
Article number:
D103-01/02/03
Number of views:
3263
Keywords:
Myco-OffTM Mycoplasma Cleaner
D103-01/02/03
58.00
Article number:
D101-01/02
Myco-BlueTM Mycoplasma Detector is a rapid detection reagent for mycoplasma contamination in cell cultures. Its core is Vazyme's unique isothermal amplification technology. Myco-BlueTM Mycoplasma Detector is very easy to use, just add 1 μl of cell culture supernatant to the reaction system and incubate at 60°C for 1 h. If the cell culture is contaminated by mycoplasma, the conservative sequence of mycoplasma DNA will be specifically amplified by isothermal DNA polymerase, which will change the reaction solution from purple to sky blue. The result can be judged by visual observation. There is no need for a PCR machine/qPCR machine, no electrophoresis, and it can be done easily in the cell room. It is very suitable for daily mycoplasma detection in biopharmaceutical companies, vaccine manufacturers, monoclonal antibody manufacturers, cell therapy/embryo laboratories and scientific research laboratories.   Take 1 μl of culture supernatant for direct detection without sample pretreatment; the product does not need electrophoresis to avoid false positives; the color is clearly distinguished, and there are no weak positives/false negatives; the accuracy is comparable to qPCR method, better than PCR method   Store at -20℃
58.00
Article number:
D101-01/02
Number of views:
5379
Keywords:
99.00
Article number:
A311-01/02
CCK-8 Cell Counting Kit, referred to as CCK-8 kit, is dependent on WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-Disulfobenzene)-2H-tetrazole monosodium salt) is a fast, highly sensitive, non-radioactive colorimetric detection kit widely used in cell proliferation and cytotoxicity detection. WST-8 is electronically coupled The carrier 1-Methoxy PMS can be reduced by some dehydrogenases in the mitochondria to form a highly water-soluble orange-yellow formazan product (Formazan). The color of the formazan produced is directly proportional to cell proliferation and inversely proportional to cytotoxicity. . Use a microplate reader to measure the absorbance at 450 nm wavelength, which can indirectly reflect the number of living cells. The CCK-8 Solution of this kit is a ready-to-use reagent, which can be directly added to the cell sample and can be tested after incubating for a certain period of time. There is no need to pre-prepare various components.    Simple operation; high sensitivity; low cytotoxicity   Store at 4℃ and avoid light
99.00
Article number:
A311-01/02
Number of views:
4411
Keywords:
145.00
Article number:
A211-01/02
145.00
Article number:
A211-01/02
Number of views:
6540
Keywords:
Annexin V-FITC/PI Apoptosis Detection Kit
A211-01/02
258.00
Article number:
A113-01/02/03
This kit uses the TUNEL (TdT mediated dUTP Nick End Labeling) method to catalyze the incorporation of terminal deoxyribonucleotide transferase (TdT) at the 3'-hydroxyl (3'-OH) end of the broken DNA of apoptotic cells Tetramethylrhodamine-deoxyuridine triphosphate (TMR red-dUTP). The kit optimizes the labeling reaction, using the best ratio of TMR red-dUTP and unlabeled dNTP to carry out the 3'-OH terminal nucleotide Incorporation, so that the end of the same broken DNA fragment can form a longer labeled tail." This "labeled tail" reduces the steric hindrance of the labeling group on the adjacent incorporated dNTPs and increases the fluorescence on each broken fragment The number of groups reduces the aggregation and quenching that may be caused by adjacent fluorescent groups, thereby improving detection sensitivity and reducing non-specific reactions. The BrightRed Labeling Mix in the kit contains TMR red-dUTP and the patented Bright factor. This unique The small molecule compound of TMR red can be non-covalently combined with TMR red to enhance its stability and amplify its signal, thereby making the marker brighter and having stronger anti-quenching ability.   The patented Bright factor makes the fluorescence brighter and the anti-quenching ability stronger; Label red fluorescence, which is convenient for co-labeling with other green fluorescence such as GFP; Highly active recombinant TdT enzyme ensures the efficiency of fluorescence incorporation; Suitable for cell slides, paraffin sections and frozen sections    Store at -20℃; Store BrightRed Labeling Mix at -20℃ in the dark
258.00
Article number:
A113-01/02/03
Number of views:
3475
Keywords:
TUNEL BrightRed Apoptosis Detection Kit
258.00
Article number:
A112-01/02/03
TUNEL BrightGreen Apoptosis Detection Kit is an upgraded version of TUNEL FITC Apoptosis Detection Kit (A111). The BrightGreen Labeling Mix in the kit contains FITC-12-dUTP and the patented Bright Factor. This unique small molecule compound can be non-covalently combined with FITC to enhance its stability and amplify its signal, thereby making the label brighter and having stronger anti-quenching ability. BrightGreen can keep strong fluorescence intensity under continuous irradiation of strong laser for 6 min.   The patented Bright factor makes the fluorescence brighter and the anti-quenching ability stronger; Highly active recombinant TdT enzyme ensures the efficiency of fluorescence incorporation; Suitable for cell slides, paraffin sections and frozen sections    Store at -20℃; Store BrightGreen Labeling Mix at -20℃ in the dark
258.00
Article number:
A112-01/02/03
Number of views:
3457
Keywords:
TUNEL BrightGreen Apoptosis Detection Kit
A112-01/02/03
229.00
Article number:
A111-01/02/03
VAHTSTM AmpSeq Adapters for Illumina is a dedicated kit for the construction of AmpSeq technology for Illumina high-throughput sequencing platform libraries. Used in conjunction with the AmpSeq library construction kit, it can construct a multi-sample targeted sequencing DNA library suitable for the Illumina platform. Kit NA111-01 contains 12 different index connectors from Adapter 1 to Adapter 12; Kit NA111-02 contains 12 different index connectors from Adapter 13 to Adapter 24; Kit NA111-03 contains 24 types from Adapter 25 to Adapter 48 Different index adapters; kit NA111-04 contains 24 different index adapters from Adapter 49 to Adapter 72; kit NA111-05 contains 24 different index adapters from Adapter 73 to Adapter 96. All adapters provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.   Store at -30 ~ -15℃
229.00
Article number:
A111-01/02/03
Number of views:
3291
Keywords:
TUNEL FITC Apoptosis Detection Kit

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