LOGO
Search
Submit
Cancel
这是描述信息
/
/
/
Nucleic Acid Isolation

NGS Library Prep kits

Molecular Research

Drug Discovery

Nucleic Acid Isolation

77.00
Article number:
EN401-01/02
77.00
Article number:
EN401-01/02
Number of views:
1000
Keywords:
117.00
Article number:
DC112-01/02
This kit is mainly suitable for extracting genomic DNA from a variety of fresh or frozen anticoagulant blood, cells, animal tissues and bacterial samples. The kit uses silica gel membrane purification technology, no need to use toxic reagents such as phenol, chloroform, and time-consuming alcohol precipitation during the extraction process, and to maximize the removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as restriction digestion, PCR, and Southern hybridization.   High quality and good integrity: The extracted DNA has the characteristics of high yield, good purity, and complete fragments. Wide range of samples: suitable for direct extraction of DNA from a variety of fresh or frozen anticoagulated blood, cells, animal tissues and bacterial samples. Simple and fast: Animal tissue provides a 30min quick extraction plan.     Proteinase K is stored at 2-8°C, and other components are stored at room temperature (15-25°C).
117.00
Article number:
DC112-01/02
Number of views:
1458
Keywords:
bacteria
and
the
of
dna
extraction
animal
high
for
extracted
115.00
Article number:
R504-01
RNase and nucleic acid scavenger, a non-toxic spray reagent, used to remove RNase contamination on the surface of instruments, equipment, and experimental desktops. At the same time, it can establish an RNA-related experimental operating environment for nucleic acid templates without affecting the stability of RNA Sex.   High removal efficiency: can completely remove nucleic acid templates such as RNase and DNA/RNA in the experimental environment  Safe, non-toxic, easy to use: liquid, spray and use No damage to equipment: non-corrosive, can be sprayed directly on the surface of the instrument   Store at room temperature (15 ~ 25℃)
115.00
Article number:
R504-01
Number of views:
1286
Keywords:
rna
the
and
experimental
acid
rnase
can
of
nucleic
to
9999.00
Article number:
R701-01/02
RNA-easy Isolation Reagent is widely used to extract Total RNA and Small RNA from samples of various animal tissues, plant materials, cultured cells and microorganisms. Compared with the traditional Trizol extraction method, this product is simple to use, does not require the use of chloroform for stratification, and the whole process can be performed at room temperature; in addition, this product effectively inhibits the activity of RNase (RNase) while simultaneously removing protein and DNA. , Polysaccharides and other impurities precipitate to the bottom of the tube to achieve single-phase extraction and effectively ensure the integrity and purity of RNA. The entire extraction process can be completed within 50 minutes using this product. The extracted RNA can be directly used in various molecular biology such as cDNA cloning, qRT-PCR detection, mRNA purification, in vitro translation, Northern blotting hybridization, high-throughput sequencing, etc. experiment.   Broad platform compatibility: The addition of universal ROX to miRNA Universal SYBR® qPCR Master Mix enables mix to be compatible with qPCR instruments on different platforms without the need to adjust ROX concentration.   Store at 2 ~ 8℃.     
9999.00
Article number:
R701-01/02
Number of views:
4294
Keywords:
reagent
the
to
and
of
be
in
extraction
rna
this
9999.00
Article number:
P073-01/02/03
RoomTemp TM Sample Lysis Kit is a simple and fast blood lysis kit at room temperature. This kit contains two components, Lysis Buffer and Stabilizing Buffer. Lysis Buffer contains special components that quickly destroy cell membrane proteins and membrane structure, which can fully release the genomic DNA in the cell. Stabilizing Buffer adds protective proteins and stabilizing factors. Eliminate the inhibitory effect of inhibitors in the lysed sample on downstream qPCR and PCR reactions, so that the lysed DNA solution can be stored stably for a long time. Applicable blood sample types include fresh blood, frozen blood and conventional anticoagulant blood (EDTA, citrate, heparin sodium, etc.). After lysis at room temperature for 3 minutes, genomic DNA can be released from the whole blood sample. The lysed DNA solution can be directly used as a template for SNP detection by Taqman ® probe method, qPCR probe method quantification, PCR amplification, etc. Without complicated extraction operations, it can achieve the same effect as traditional genome extraction methods. In addition to blood, this kit is also compatible with pyrolysis FTA blood cards, buccal swabs, plant tissues and other samples.   Simple operation: 3 minutes at room temperature, no need for complex template extraction. High lysis efficiency: The lysis reagent has the same effect as traditional kits for extracting genome. Wide range of applications: Compatible with Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification and other sample compatibility: lysis of fresh blood, frozen blood, conventional anticoagulant blood, tissue homogenate, oral swabs, Various samples such as FTA card.   Store at 2~8℃.
9999.00
Article number:
P073-01/02/03
Number of views:
2708
Keywords:
roomtemp
blood
the
lysis
and
for
can
dna
method
as
99.00
Article number:
DC111-01/02
This kit is mainly suitable for quickly and easily extracting genomic DNA from 0.1-1 ml fresh and frozen anticoagulated whole blood samples. The kit uses silica gel membrane purification technology, and no toxic reagents such as phenol and chloroform are required during the extraction process. There is no need for time-consuming alcohol precipitation, and the maximum removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as enzyme digestion, PCR, Southern blotting, etc.   High yield, excellent purity, good integrity; wide range of sample application; wide range of volume compatibility Except for Proteinase K, the other components of the kit should be stored at 15-25°C; Proteinase K can be stored at 15-25℃ for three months, long-term storage needs to be placed at 2-8℃.  
99.00
Article number:
DC111-01/02
Number of views:
2928
Keywords:
mini
and
the
for
be
of
at
kit
high
dna
127.00
Article number:
DC105
This product is suitable for separation and purification of genomic DNA from formalin-fixed and paraffin-embedded tissues, and overcomes the inhibitory effect caused by formalin cross-linking. The dewaxing solution used in this kit is safer than xylene; and high-quality DNA can be obtained through a centrifugal adsorption column that specifically binds DNA and a unique buffer system. The entire extraction process only takes 20 minutes (except the digestion time). The extracted genome has good integrity, high purity, stable and reliable quality, and can be used in a variety of downstream applications, such as PCR, qPCR, library construction and other experiments.   safe and non-toxic; fast; Efficient high production   The components of this product except Proteinase K can be stored at 15 ~ 25℃ for 12 months. If you need to store it for a longer time, store it at 2 ~ 8℃. If the solution precipitates under storage conditions of 2 ~ 8℃, the solution in the kit should be placed at room temperature for a period of time before use, and turned upside down until the precipitate is completely dissolved; if necessary, preheat it in a 37℃ water bath for 10 minutes to dissolve the precipitate.
127.00
Article number:
DC105
Number of views:
2408
Keywords:
dna
the
and
for
of
in
be
if
is
17.00
Article number:
DE103
Lysozyme (lysozyme) is an alkaline enzyme that can hydrolyze mucopolysaccharides in bacteria. It mainly destroys the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall. The cell wall insoluble mucopolysaccharide is decomposed into soluble glycopeptides, causing the cell wall to rupture and the bacteria to dissolve. Mainly used for the digestion of the cell wall of gram-positive bacteria during the preparation of DNA and RNA.   High yield, excellent purity, good integrity; wide range of sample application; wide range of volume compatibility   Except for Proteinase K, the other components of the kit should be stored at 15-25°C; Proteinase K can be stored at 15-25℃ for three months, long-term storage needs to be placed at 2-8℃.  
17.00
Article number:
DE103
Number of views:
2196
Keywords:
lysozyme
the
of
cell
at
be
for
and
to
wall
171.00
Article number:
RC401
This kit can quickly extract the total RNA of multiple samples from plant tissues, especially plant tissues rich in polysaccharides and polyphenols (such as cotton leaves, pine needles, bananas, potato tubers, grapes, rose flowers, buckwheat, etc.). The kit is based on silica gel column purification technology. There is no need to use toxic phenol-chloroform extraction during the extraction process. The whole process only takes 30 minutes. The FastPure gDNA-Filter Column II in the kit can effectively filter impurities and specifically adsorb and remove genomic DNA. FastPure RNA Column Ⅳ can efficiently bind RNA, and is equipped with optimized Buffer solution, so that the total RNA obtained is of high purity, free of protein and other impurities, and can be used for RT-PCR, Real-time PCR, chip analysis, Northern Blot And other downstream experiments.   Safety; fast; Wide compatibility; High purity; Less genome residue   Store at room temperature (15-25℃)
171.00
Article number:
RC401
Number of views:
4178
Keywords:
polyphenolics
the
and
is
rna
can
column
kit
of
tissues
116.00
Article number:
DC202
This kit is suitable for extracting 150-300 ml of overnight cultured bacteria, using an improved SDS-alkaline lysis method to lyse the bacteria, the crude extract is selectively combined with a unique endotoxin scavenger and centrifuged to remove endotoxins, and then The silicon matrix membrane in the centrifugal adsorption column selectively binds the plasmid DNA in the solution under high salt and low pH conditions, and then removes impurities and other bacterial components through the rinsing solution, and finally elution with low salt and high pH The buffer eluted purified plasmid DNA from the silicon matrix membrane. The silicon matrix membrane in the centrifugal adsorption column all adopts imported special adsorption membrane, the adsorption amount difference between the column and the column is very small, and the repeatability is good. There is no need to use toxic phenol, chloroform and other reagents, nor ethanol precipitation. It can quickly extract 0.2-1.5 mg of pure high-copy plasmid DNA with an extraction rate of 80-90%. The unique process formula removes endotoxin, the content of endotoxin is very low (< 0.1 EU/μg DNA), and the cell transfection effect is excellent. It can also be directly used in various molecular biology experiments such as enzyme digestion, PCR, in vitro transcription, transformation, sequencing, and so on.   High Yield; Compatible with the extraction of plasmids of different lengths; The content of endotoxin is extremely low, and subsequent transfection level experiments can be done; Good stability   RNase A should be stored at -30 ~ -15℃; The endotoxin scavenger can be stored at 2-8℃ for one month, please store at -30 ~ -15℃ for long-term storage; The other components of the kit are stored at room temperature (15 ~ 25℃). 
116.00
Article number:
DC202
Number of views:
5085
Keywords:
endofree
the
and
is
of
in
endotoxin
dna
at
be
96.00
Article number:
DC103
This kit is suitable for extracting genomic DNA from various sources of bacteria (Gram-negative bacteria, Gram-positive bacteria). The kit uses silica gel membrane purification technology. There is no need to use toxic reagents such as phenol and chloroform during the extraction process, and no time-consuming alcohol precipitation, and to maximize the removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as restriction digestion, PCR, and Southern hybridization.   Simple and fast; Wide range of applications; High yield and good integrity; High purity   RNase A and Proteinase K dry powder should be stored at -30~-15℃; Store other components at room temperature (15-25℃)    
96.00
Article number:
DC103
Number of views:
2976
Keywords:
75.00
Article number:
DC104
This kit is suitable for extracting genomic DNA from fresh and dried common plant samples or polysaccharide polyphenol plant samples. The kit uses silica gel membrane purification technology and a new and unique solution system. There is no need to use toxic reagents such as phenol, chloroform, or time-consuming alcohol precipitation during the extraction process, which can maximize the removal of impurity proteins and other organic compounds in plant cells. The extracted genomic DNA has high purity and stable quality, and can be used in molecular biology experiments such as PCR, enzyme digestion and hybridization.   Simple and fast; Wide range of applications; High yield and good integrity; High purity   RNase A is stored at -30~-15℃; Store other components at room temperature (15-25℃)
75.00
Article number:
DC104
Number of views:
2988
Keywords:
mini
and
the
high
is
plant
dna
in
as
at
308.00
Article number:
RC201
MiPure Cell / Tissue miRNA Kit is suitable for extracting small RNA (miRNA, <200 nt) from less than 5 x 106 eukaryotic cells or less than 100 mg animal tissues. The resulting miRNA has high purity without large RNA or genome DNA contamination can be directly used for chip analysis, Northern hybridization, RT-PCR and miRNA library construction. This kit combines high-efficiency RNA Isolater extraction reagents and silica gel column purification technology to complete the extraction of miRNA within 1 hour; it can also be used to separate and extract large molecular RNA (>200 nt) or total RNA (including miRNA) to satisfy users Different needs.   The miRNA extraction efficiency is high, and the starting amount is wide; Larger RNA and genomic DNA have fewer residues; Easy to operate, strong compatibility   Box 1: Store at 4℃ and avoid light Box 2: Store at room temperature
308.00
Article number:
RC201
Number of views:
3706
Keywords:
RC201
162.00
Article number:
RC101
This kit can quickly extract total RNA from animal cells or tissues. The kit is based on silica gel column purification technology. There is no need to use toxic phenol/chloroform extraction during the extraction process. The whole process only takes 30 minutes. The gDNA-Filter Columns in the kit can effectively separate the supernatant and adsorb and remove gDNA; RNAPure Columns can efficiently bind RNA, with optimized Buffer solution, so that the total RNA obtained is of high purity, free of protein and other impurities, and can be used Used in various downstream experiments such as RT-PCR, Real-Time PCR, chip analysis, etc.   High purity; Few genome residues; Simple and fast Strong versatility   DNase I is stored at -20℃; Buffer RL1 can be stored at 4℃ for 1 month after adding β-mercaptoethanol; The remaining components can be stored under dry conditions at room temperature (15-25°C). 
162.00
Article number:
RC101
Number of views:
6504
Keywords:
RC101
678.00
Article number:
R603
VEXTM Exosome Isolation Reagent (from plasma) is a reagent specially used to separate and extract exosomes from plasma samples. A large number of exosomes can be separated from the sample by simple low-speed centrifugation. Compared with the traditional ultra-high-speed centrifugation, this product has less pressure on the exosomes in the sample and can maintain a more complete shape; at the same time, the extraction process requires a shorter time and requires a lower initial sample volume. , The extraction efficiency is higher. The exosomes obtained by using this product can be applied to a variety of downstream applications, such as protein research, RNA analysis, high-throughput sequencing, etc.   The operation is simple and fast; Compatible with low-volume samples; Stable and reliable output   VEX Exosome Isolation Reagent (from plasma) stored at 2-8℃; Proteinase K is stored at ﹣15—﹣30℃
678.00
Article number:
R603
Number of views:
2054
Keywords:
R603
678.00
Article number:
R602
VEXTM Exosome Isolation Reagent (from serum) is a reagent specially used to separate and extract exosomes from serum samples. A large number of exosomes can be separated from the sample by simple low-speed centrifugation. Compared with the traditional ultra-high-speed centrifugation, this product has less pressure on the exosomes in the sample and can maintain a more complete shape; at the same time, the extraction process requires a shorter time and requires a lower initial sample volume. , The extraction efficiency is higher. The exosomes obtained using this product can be applied to a variety of downstream applications, such as protein research, RNA analysis, high-throughput sequencing, etc.   The operation is simple and fast; Compatible with low-volume samples; Stable and reliable output   Store at 2-8℃
678.00
Article number:
R602
Number of views:
2001
Keywords:
R602
613.00
Article number:
R601
VEXTM Exosome Isolation Reagent (from cell culture media) is a reagent specially used to separate and extract exosomes from cell culture supernatant samples. A large number of exosomes can be separated from the sample by simple low-speed centrifugation. Compared with the traditional ultra-high-speed centrifugation, this product has less pressure on the exosomes in the sample and can maintain a more complete shape; at the same time, the extraction process requires a shorter time and requires a lower initial sample volume. , The extraction efficiency is higher. The exosomes obtained by using this product can be adapted to a variety of downstream applications, such as RNA analysis, high-throughput sequencing, cell co-culture, etc.   The operation is simple and quick; Compatible with low-volume samples; Stable and reliable output   Store at 4°C
613.00
Article number:
R601
Number of views:
2339
Keywords:
87.00
Article number:
R502-01
RNA Keeper-ICE Tissue Transition Buffer is a new type of reagent that can transform frozen tissue into a state that can be easily processed by ordinary homogenization methods to extract high-quality RNA. It eliminates the need to grind the frozen tissue into powder before homogenization, and even further cut the tissue before homogenization. In the process of treating with RNA Keeper-ICE solution, when the tissue changes from a hard frozen state to a soft state, the solution penetrates into the tissue and inactivates the RNase. Once the tissue is treated with RNA Keeper-ICE solution, it can be operated at room temperature without worrying about RNA degradation. The sample can be safely weighed, further cut, or subjected to various experiments without affecting the quality of RNA. The sample can be directly added to the lysis solution to be broken, or stored at -20°C for a long time.   Superior reaction system: the most suitable Buffer composition and concentration, more suitable for microRNA reverse transcription and qPCR experiments;   Store at room temperature
87.00
Article number:
R502-01
Number of views:
2222
Keywords:
65.20
Article number:
R501-01
RNA Keeper Tissue Stabilizer is a non-toxic solution that can quickly penetrate into tissues, inactivate endogenous RNase, immediately stabilize and protect the integrity of RNA. Fresh tissue samples do not need liquid nitrogen quick freezing, as long as they are immersed in RNA Keeper Tissue Stabilizer, they can be stored at 37°C for 1 day, room temperature for 1 week, 4°C for 4 weeks, or long-term storage at -20°C/-80°C, and repeated Freezing and thawing will not significantly affect the integrity of RNA. The samples stored in the RNA Keeper Tissue Stabilizer can be directly used to extract RNA using RNA isolater total RNA extraction reagent (R401-01) or spin column method. RNA Keeper Tissue Stabilizer can be used for the preservation of brain, heart, liver, pancreas, kidney, spleen, testis, muscle and other tissues.   Ultra-high amplification specificity: AceTaq® DNA Polymerase based on chemical hot-start, completely blocked enzyme activity before 95°C, and equipped with a patented specific promotion factor Exactor, which makes amplification more specific;   Store at room temperature
65.20
Article number:
R501-01
Number of views:
2846
Keywords:
69.00
Article number:
R403-01
Bacteria RNA Extraction Kit is a kit optimized for RNA extraction from prokaryotes (including Gram-negative and Gram-positive bacteria). This kit includes Bacteria RNA Plus Reagent containing optimized buffer system, chelating agent and mild protein denaturant, and RNA isolater Total RNA Extraction Reagent that can effectively inhibit endogenous RNase and promote protein denaturation. The combination of the two can greatly increase the yield of prokaryotic RNA extraction while ensuring good product quality. When extracting bacterial RNA, only one step of high temperature pretreatment is added before routine operations, without mechanical disruption or enzymatic digestion steps, and high-quality and yielding bacterial total RNA can be obtained. This kit is also suitable for extraction of total RNA from yeast and other fungi.   Targeted optimization for the extraction of total RNA from prokaryotes (gram-positive bacteria and gram-negative bacteria) and fungi (non-spores); The pretreatment solution is used in conjunction with the guanidine isothiocyanate-based lysis solution to greatly increase the RNA yield and purity; Simple operation, time saving, no need for lysozyme or ultrasonic treatment   RNA Isolater Total RNA Extraction Reagent is stored at 4℃ and protected from light; Bacteria RNA Plus Reagent is stored at room temperature
69.00
Article number:
R403-01
Number of views:
2986
Keywords:
37.50
Article number:
R402-01
Bacteria RNA Plus Reagent is an auxiliary reagent optimized for RNA extraction from prokaryotes (including Gram-negative and Gram-positive bacteria). It can be used with Vazyme RNA Isolater Total RNA Extraction Reagent (R401-01) to greatly improve the RNA extraction of prokaryotes. Yield, while ensuring good product quality. Bacteria RNA Plus Reagent contains an optimized buffer system, chelating agents and mild protein denaturants. When extracting bacterial RNA, a high-temperature pretreatment is added before routine operations. Together with Vazyme RNA Isolater Total RNA Extraction Reagent, it can effectively inhibit endogenous RNase promotes protein denaturation, thereby increasing the yield and quality of bacterial RNA. This reagent is also suitable for extracting RNA from yeast and other fungi.   Greatly increase the amount of total RNA extracted from bacteria (gram-negative bacteria and gram-positive bacteria). To ensure good quality of RNA products, it is easier to meet the needs of subsequent experiments. The operation is simple, time-saving, no need for lysozyme or ultrasound and other breaking treatments, eliminating the RNA degradation that may be introduced by the operation. No bacterial genomic DNA contamination.   Store at room temperature
37.50
Article number:
R402-01
Number of views:
2123
Keywords:
42.00
Article number:
R401-01
RNA isolater Total RNA Extraction Reagent, based on guanidine isothiocyanate and phenol, has a strong lysis ability, which can lyse cells and tissue samples in a short time to ensure the integrity of RNA. This product is widely used for culturing cells, animal tissues, microorganisms, and plant tissues with less secondary metabolism, such as seedlings and young leaves. The sample can be fully lysed in RNA isolater. After centrifugation with chloroform, the solution will form a supernatant layer, an intermediate layer and an organic layer (bright red lower layer). RNA is distributed in the upper aqueous phase. After the supernatant layer is collected, the Total RNA can be recovered by propanol precipitation.   Based on guanidine isothiocyanate and phenol, strong cracking ability; The optimized lysate formula has a wide range of sample adaptability.    Store at 4℃ and avoid light
42.00
Article number:
R401-01
Number of views:
7097
Keywords:
36.00
Article number:
DC301
This kit uses an optimized buffer system and silica gel column purification technology, which can recover 70 bp-20 kb DNA fragments from various concentrations of TAE or TBE agarose gels, and transfer them to the DNA adsorption column under high salt conditions. Direct centrifugation can specifically adsorb DNA and remove other impurities. In addition, this kit can directly purify DNA fragments from PCR products, enzymatic reaction systems or crude DNA products obtained by various other methods, while removing impurities such as proteins, other organic compounds, inorganic salt ions, and oligonucleotide primers. . It can ensure that the purification work is completed in 10-15 min. The purified DNA can be directly used for molecular biology research such as ligation, transformation, restriction enzyme digestion, in vitro transcription, PCR, sequencing, and microinjection.   Simple and fast; One box for dual use; High recovery efficiency; Wide range of recovered DNA fragments   Store at room temperature (15-25℃)
36.00
Article number:
DC301
Number of views:
7359
Keywords:
DC301
96.00
Article number:
DC102
This product is suitable for the extraction of genomic DNA from 1-20 mg animal tissues and cultured cell samples less than 5 × 106. The kit is based on silica gel column purification technology. There is no need to use phenol/chloroform organic solvent extraction and alcohol precipitation during the extraction process. It only takes 30-60 minutes to obtain high-purity and high-yield genomic DNA, which can be directly used in the enzyme digestion reaction. , PCR, qPCR, library construction and Southern hybridization experiments.   Simple and fast; Wide adaptability; High purity; High security   Proteinase K dry powder and RNase Solution should be stored at -20°C, and the dissolved Protein K solution should be aliquoted and stored at -20°C; Other components (store at 15-25°C). 
96.00
Article number:
DC102
Number of views:
4169
Keywords:

The products on this website may not be sold in all countries or regions. Please contact Vazyme or your local distributor for further information.

这是描述信息

Address: Floor 1-3, Building C2, Red Maple Technology Industrial Park, Kechuang Road Economy & Technology Development Zone, Nanjing, China.

Copyright 2015 Vazyme Biotech Co.,Ltd All rights reserved  苏ICP备12014779号-1  Power by: www.300.cn nanjing.300.cn