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Reverse Transcription

NGS Library Prep kits

Molecular Research

Drug Discovery

Reverse Transcription

1200.00
Article number:
HF001-1 
HiscriptHighFidelityOneStepRT-PCRKit一步法高效RT-PCR试剂盒逆转录和PCR在同一管内进行检测灵敏度可达0.1pg总RNA可扩增超过10kb的长片段图1A.以Hela细胞总RNA为模板,用Hiscript® HighFidelityOneStepRT-PCRKit (HF001)扩增555bpGAPDH片段。检测灵敏度可达到0.1pg。B.以猪蓝耳病毒PRRSVRNA为模板,用Hiscript®HighFidelityOneStepRT-PCRKit(HF001)扩增660bp片段。检测灵敏度可达到0.2TCLD50。C.以1μgHela细胞总RNA为模板,用Hiscript®HighFidelityOneStepRT-PCRKit(HF001)扩增不同长度的片段。可成功扩增长达12.1kb的UTRN片段。本试剂盒专为以RNA为模板(如RNA病毒)的终点法PCR检测而设计。使用基因特异引物(GSP),逆转录和PCR反应在一管内完成,不需要额外的开管/移液操作,大大提高了检测通量,并降低了污染的风险。整合HiScript®IIReverseTranscriptase以及ChampagneTaqTMDNAPolymerase的优越性能,配合经过优化的缓冲体系,Hiscript®HighFidelityOneStepRT-PCRKit(HF001) 可扩增片段长达10kb以上。试剂盒以便捷的MasterMix形式提供。2×OneStepMix包含优化的缓冲体系和dNTPs;OneStepEnzymeMix包含比例优化的HiScript®IIReverseTranscriptase、RNaseinhibitor以及ChampagneTaqTM plusDNAPolymerase,操作简单快捷。组分HF001-0150rxn(50ul/rxn)RNasefreeddH2O1ml×22×OneStepMixa625μl×2OneStepEnzymeMixb125μl10×Loadingbuffer1.25mla.包含dNTP和缓冲体系b.包含HiScript® IIReverseTranscriptase、RNaseinhibitor以及ChampagneTaqTM plusDNAPolymerase-30~-15℃保存。
1200.00
Article number:
HF001-1 
Number of views:
4965
Keywords:
fidelity
one
step
hiscript
片段
rt-pcr
扩增
rna
kit
检测
HF001-01
HF001
hf001
242.00
Article number:
R323-01
HiScript® III RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript® II Q RT SuperMix for qPCR (+gDNA wiper), including a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the optimal buffer optimized for reverse transcription further improve the efficiency of cDNA synthesis and are suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the kit can completely remove the remaining genomic DNA in the RNA template, ensure that the subsequent quantitative results are more reliable, and simplify the design of qPCR primers without the need to design primers across introns; 5 × HiScript III qRT SuperMix contains All components required for reverse transcription reaction can be reacted quickly by adding template RNA and water, and at the same time, the gDNA wiper function is terminated to ensure the integrity of cDNA. The reverse transcription product is compatible with dye method and probe method qPCR, which can perform high-performance gene expression analysis.    Easy and quick operation: 5 × HiScript III qRT SuperMix contains all the components required for reverse transcription reaction, only need to add template RNA, the reverse transcription reaction can be completed within 20 minutes; Extensive template compatibility: Compatible with RNA templates of different species and poor integrity; Super impurity tolerance: It has super tolerance to common reverse transcription impurities such as ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid, etc.; Excellent reverse transcription efficiency: Compared with common commercial reverse transcription products, HiScript ® III RT SuperMix for qPCR (+gDNA wiper) reverses the smallest cDNA quantitative C T value and has excellent reverse transcription efficiency.   Store at -30~-15°C
242.00
Article number:
R323-01
Number of views:
13662
Keywords:
gdna
the
reverse
transcription
and
for
hiscript
of
supermix
iii
174.00
Article number:
R312-01/02
HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the most suitable for reverse transcription optimization. Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across introns. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR quantification.   Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product; Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs; gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns    Store at -30~-15°C (restore 10*RT Mix to room temperature and shake well for use)
174.00
Article number:
R312-01/02
Number of views:
8935
Keywords:
synthesis
the
reverse
primers
for
and
of
to
can
transcription
144.00
Article number:
R302-01
144.00
Article number:
R302-01
Number of views:
3986
Keywords:
hiscript
reverse
transcription
of
the
can
transcriptase
super
impurity
203.00
Article number:
P621-01
The Single Cell Squence Specific Amplification Kit is based on a one-step RT-PCR amplification method, which is used to realize the amplification of the transcriptome in single cells or trace amounts of total RNA, so that you can uncover the expression levels of different genes between individual cells. This kit realizes that RNA extraction and purification, reverse transcription and PCR reactions are completed in the same tube, no additional operations are required, and it has the advantages of saving time, reducing experimental errors, reducing pollution, and improving sensitivity. This kit is also suitable for one-step expansion of 2-1000 cells, and the number of cycles of one-step expansion should be reduced according to the number of cells.   Suitable for 1-10,000 cells; One-step RT-PCR pre-amplification; Up to 500 target genes can be amplified; The amplified product is suitable for analysis by any real-time fluorescent quantitative PCR instrument; High sensitivity, low cost    Store at -20℃
203.00
Article number:
P621-01
Number of views:
3330
Keywords:
single
the
of
and
is
one-step
amplification
to
for
kit
123.00
Article number:
MR101-01/02
miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) is a special kit suitable for stem-loop miRNA cDNA one-strand synthesis. It contains genomic DNA removal steps, which can quickly remove genomic DNA contamination at 42℃ for 2 min. The follow-up results are more reliable. The HiScript ® II Reverse Transcriptase on which the kit is based has extremely high thermal stability, coupled with an optimized buffer system, which is conducive to the generation of miRNA-specific reverse transcription products. For subsequent quantification of cDNA products, it is recommended to use our company's miRNA Universal SYBR ® qPCR Master Mix (Vazyme # MQ101) to obtain the best experimental results. At the same time, the design software for miRNA reverse transcription stem-loop primers and quantitative primers was launched, which is easy to operate and escort miRNA experiments.   Excellent linear relationship: It has a good linear relationship in a wide template interval, and can detect RNA templates as low as pg; Superior reaction system: Optimal Buffer composition and concentration, more suitable for reverse transcription of microRNA; Simple primer design: Provide matching primer design software to make primer design easier   Store at -20℃
123.00
Article number:
MR101-01/02
Number of views:
6378
Keywords:
MR101-01/02
MR101
119.00
Article number:
P612-01
HiScript® II One Step RT-PCR Kit (Dye Plus) is specially designed for end-point PCR detection using RNA as a template (such as RNA virus). PCR products can be directly loaded for electrophoresis, which greatly saves time and labor. Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which improves detection throughput and reduces the risk of contamination. Integrating the superior performance of HiScript® II Reverse Transcriptase and Champagne TaqTM plus DNA Polymerase, with an optimized buffer system, the detection sensitivity of HiScript® II One Step RT-PCR Kit (Dye Plus) can reach 1 pg total RNA and can be amplified Fragments up to 10 kb. The kit is provided in a convenient Master Mix format. 2 × One Step Mix (Dye Plus) contains optimized buffer system, dNTP and loading dye; One Step Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.   Reverse transcription and PCR are performed in the same tube; The detection sensitivity can reach 0.1 pg total RNA; Amplify long fragments up to 10 kb or more; PCR products can be directly loaded for electrophoresis   Store at -20℃
119.00
Article number:
P612-01
Number of views:
4855
Keywords:
P612-01
119.00
Article number:
P611-01
HiScript® II One Step RT-PCR Kit is specially designed for end-point PCR detection using RNA as template (such as RNA virus). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. Integrate HiScript® II Reverse Transcriptase and the superior performance of Champagne TaqTM plus DNA Polymerase designed for long fragment amplification, and cooperate with the optimized buffer system HiScript® II One Step RT-PCR Kit to amplify fragments up to 10 kb or more. The kit is provided in a convenient Master Mix format. 2 × One Step Mix contains optimized buffer system and dNTP; One Step Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.   Reverse transcription and PCR are performed in the same tube; The detection sensitivity can reach 0.1 pg total RNA; Amplify long fragments over 10 kb   Store at -20℃
119.00
Article number:
P611-01
Number of views:
4995
Keywords:
P611-01
180.00
Article number:
R233-01
This product is based on the cost-effective HiScript ® II Reverse Transcriptase and uses uniquely designed Oligo(dT) 23 VN primers to improve the specificity and sensitivity of the reaction and obtain better quality cDNA. Different types of reverse transcription primers can be used flexibly for different experimental designs to meet diverse experimental needs. Oligo (dT) 23 VN primer/Random Hexamers or gene-specific primers (GSP) can be selected according to the needs of subsequent reverse transcription experiments. At the same time, the reaction system is greatly simplified. 5 × qRT SuperMix contains all the reagents required for reverse transcription reaction except the primers and will not freeze at -20°C, making it easy to use. The 4 × gDNA wiper Mix in the kit can completely remove residual genomic DNA contamination, ensuring that the subsequent quantitative results are more reliable. The product obtained by reverse transcription can be used in SYBR ® Green qPCR analysis method and probe analysis method for high-performance gene expression analysis.   Based on the efficient HiScript ® II Reverse Transcriptase; Different types of reverse transcription primers can be used flexibly for different experimental designs; gDNA wiper Mix can quickly and completely remove genome contamination   Store at -20℃
180.00
Article number:
R233-01
Number of views:
5377
Keywords:
R233-01
169.00
Article number:
R232-01
HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Greatly improve thermal stability and cDNA synthesis efficiency. HiScript® II Q Select RT SuperMix for qPCR is suitable for two-step qRT-PCR detection. 5 × HiScript II Select qRT SuperMix contains Buffer, dNTP Mix, HiScript II Reverse Transcriptase and RNase inhibitor. Oligo (dT) can be selected as required 23VN, Random primers or gene-specific primers are used as reverse transcription primers. Oligo (dT)23VN has stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. 5 × HiScript® II Select qRT SuperMix will not freeze at -20℃, and the reaction can be performed quickly by adding template RNA and primers. The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for use in conjunction with high-performance gene expression analysis.    Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher; Different types of reverse transcription primers can be used flexibly for different experimental designs; Ready-to-use SuperMix premix is ​​easy to use and time-saving   Store at -20℃
169.00
Article number:
R232-01
Number of views:
4421
Keywords:
R232-01
290.00
Article number:
R223-01
HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Greatly improve thermal stability and cDNA synthesis efficiency. HiScript® II Q RT SuperMix for qPCR (+gDNA wiper) is suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the RNA template product can completely remove residual genomic DNA contamination, ensuring that the subsequent quantitative results are more reliable. 5 × HiScript qRT SuperMix II contains all the components required for the reverse transcription reaction. Add template RNA and water to quickly react and terminate the gDNA wiper function to ensure the integrity of the cDNA. This product is specially optimized for qPCR. The optimized ratio of Random primers/Oligo(dT)23VN primer mix enables cDNA synthesis to start from various regions of the RNA transcript and has the same reverse transcription efficiency, ensuring the maximum qPCR results Authenticity and repeatability. The reverse transcription product is compatible with SYBR Green and probe qPCR, and the corresponding reagents can be selected according to the purpose of the experiment to perform high-performance gene expression analysis.    Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher; Anchored Oligo (dT) 23 VN is uniquely designed to ensure reaction specificity and improve the efficiency and success rate of first-strand cDNA synthesis; The ready-to-use SuperMix premix is ​​convenient and time-saving to use; gDNA wiper Mix can quickly and completely remove genome contamination   Store at -20℃
290.00
Article number:
R223-01
Number of views:
12661
Keywords:
169.00
Article number:
R222-01
HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology based on M-MLV (RNase H-) Reverse Transcriptase, which greatly improves thermal stability and cDNA synthesis efficiency. HiScript® II Q RT SuperMix for qPCR is suitable for two-step qRT-PCR detection. 5 × HiScript II qRT SuperMix contains all the components required for reverse transcription reaction. The reaction can be performed quickly by adding template RNA and water. The volume of RNA template can be added up to the total volume It is very suitable for the reverse transcription reaction of low-concentration RNA template. 5 × HiScript® II qRT SuperMix will not freeze at -20°C and is easy to use. This product is specially optimized for qPCR. Oligo (dT)23VN has stronger anchoring ability to Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. The optimized ratio of Random primers/Oligo (dT)23VN primer mix enables cDNA synthesis to start from each region of the RNA transcript and has the same reverse transcription efficiency, ensuring the authenticity and repeatability of qPCR results to the greatest extent. The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for use in conjunction with high-performance gene expression analysis.    Based on the efficient HiScript ® II Reverse Transcriptase, the reverse transcription efficiency is higher; Anchored Oligo (dT)23 VN is uniquely designed to ensure reaction specificity and improve the efficiency and success rate of first-strand cDNA synthesis; The ready-to-use SuperMix is ​​easy to use and time-saving   Store at -20℃
169.00
Article number:
R222-01
Number of views:
5380
Keywords:
9999.00
Article number:
R122-01
HiScript® Reverse Transcriptase is a new reverse transcriptase derived from M-MLV (RNase H-) Reverse Transcriptase through multiple mutations, which improves thermal stability and cDNA synthesis efficiency. HiScript® Q RT SuperMix for qPCR is suitable for both Step qRT-PCR detection, 5 × qRT SuperMix contains all the components required for reverse transcription reaction, and the reaction can be carried out quickly by adding template RNA and water. The volume of RNA template can be added up to 80% of the total volume, which is very suitable Reverse transcription reaction for low-concentration RNA template. 5×Mix will not freeze at -20°C and is easy to use. This product is specially optimized for qPCR. The optimized ratio of Random primers/Oligo dT primer mix enables cDNA synthesis to start from each region of the RNA transcript and has the same reverse transcription efficiency, ensuring the authenticity of the qPCR results to the greatest extent. Repeatability. The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for use in conjunction with high-performance gene expression analysis.    Ready-to-use SuperMix premix is ​​convenient and time-saving   Store at -20℃
9999.00
Article number:
R122-01
Number of views:
3974
Keywords:
R122-01
20.00
Article number:
R301-01/02/03
Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript® II Reverse Transcriptase, HiScript® Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).   The enhanced antioxidant capacity will not form the disulfide bonds that human-derived proteins would produce under oxidative conditions; High-purity E. coli soluble expression, no RNase residue, compatible with RT-PCR/qPCR system   Store at -20℃
20.00
Article number:
R301-01/02/03
Number of views:
3169
Keywords:
R301-01/02/03
900.00
Article number:
R212-01/02
HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper) is a special kit for cDNA one-strand synthesis that contains a genomic DNA removal step. The HiScript® II Reverse Transcriptase based on the Kit has a significant improvement compared with the previous generation HiScript® Reverse Transcriptase. Thermal stability, template affinity and inhibitor tolerance. The gDNA wiper used in the Kit can quickly remove genomic DNA contamination at 42°C for 2 minutes, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across exons. 10 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo dT23VN included in the kit has a stronger anchoring ability for Poly A+ RNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo dT23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can synthesize full-length cDNA for cloning (up to 20 kb), but also synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR   Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 10 pg-5 μg total RNA, can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.; gDNA wiper Mix can quickly and completely remove genome contamination   Store at -20℃
900.00
Article number:
R212-01/02
Number of views:
6435
Keywords:
R212-01/02
105.00
Article number:
R211-01/02
HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation of HiScript® Reverse Transcriptase, HiScript® II has further greatly improved thermal stability and is very suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript® II adds multiple point mutations, which further enhances template affinity and progress, and has higher tolerance to common reverse transcription inhibitors, which greatly improves the ability to synthesize full-length cDNA. HiScript® II 1st Strand cDNA Synthesis Kit is a first-strand cDNA synthesis kit based on HiScript® II Reverse Transcriptase. It contains all the components needed to synthesize high-quality first-strand cDNA. The product is suitable for subsequent PCR, qPCR and other experiments. 2 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo (dT)23VN included in the kit has a stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo (dT)23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can not only synthesize full-length cDNA (up to 20 kb) for cloning, but also efficiently synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR.   Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 1 pg-5 μg total RNA, and can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.   Store at -20℃
105.00
Article number:
R211-01/02
Number of views:
5528
Keywords:
R211-01/02
31.88
Article number:
R201-01/02
HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation HiScript® Reverse Transcriptase, HiScript® II Transcriptase has further greatly improved thermal stability, with a half-life of more than 4 hours at 50°C, and can be reacted for a long time at 55°C to maintain stability. It is very suitable for complex secondary Reverse transcription of structural RNA template. In addition, HiScript® II Transcriptase adds a number of point mutations, which further enhances template affinity and progression, greatly improving the synthesis ability of full-length cDNA, and obtaining cDNA up to 20 kb. It has a higher tolerance to common reverse transcription inhibitors and is very suitable for the reverse transcription reaction of plant tissue RNA rich in polysaccharides and polyphenols.   The half-life at 50℃ exceeds 240 min, and the half-life at 55℃ can reach 60 min; CDNA up to 10 kb can be synthesized in 5 minutes, which is suitable for rapid reverse transcription reaction; Higher impurity tolerance and higher detection sensitivity, reverse transcriptase is the first choice for molecular diagnosis   Store at -20℃
31.88
Article number:
R201-01/02
Number of views:
4621
Keywords:
R201-01/02
9999.00
Article number:
R111-01/02
HiScript® Reverse Transcriptase is a brand-new reverse transcriptase obtained through multiple mutations on the basis of M-MLV (RNase H-) Reverse Transcriptase. HiScript® Reverse Transcriptase has the highest activity at 50°C and can withstand reaction temperatures as high as 55°C. It can be used for reverse transcription of RNA templates with secondary structure. By enhancing the affinity with the template, more full-length cDNA can be obtained, and the total RNA can be detected as low as 1 pg. It is especially suitable for reverse transcription of a small number of templates and low-copy genes. In addition, the mismatch rate of HiScript® Reverse Transcriptase is also lower than that of M-MLV (RNase H-) Reverse Transcriptase, which improves the fidelity of cloning. The HiScript® First-strand cDNA Synthesis Kit is based on HiScript® Reverse Transcriptase and contains all the ingredients needed to synthesize high-quality first-strand cDNA, suitable for subsequent PCR, qPCR and PCR cloning. 2 × RT Mix contains optimized buffer system and dNTP; Enzyme Mix contains HiScript®Reverse Transcriptase and RNase inhibitor. Oligo (dT)18, Random hexamers, or gene-specific primers can be selected as reverse transcription primers according to needs.    HiScript ® Reverse Transcriptase provides reliable reverse transcription performance; A wide range of template amount, which can be successfully reverse transcribed from 10 pg-5 μg total RNA; Efficient synthesis ability: can synthesize up to 10 kb cDNA; Different primers can be selected for different downstream applications. The synthesized one-strand cDNA can be widely used in molecular cloning, hybridization, PCR amplification, and qPCR reactions.    Store at -20℃
9999.00
Article number:
R111-01/02
Number of views:
3922
Keywords:
R111-01/02
9999.00
Article number:
R101-01/02
HiScript® Reverse Transcriptase is a new reverse transcriptase based on M-MLV (RNase H-) Reverse Transcriptase through multiple mutations. HiScript® Reverse Transcriptase has the highest activity at 50°C and can withstand reaction temperatures up to 55°C, and can be used for reverse transcription of RNA templates with secondary structure. By enhancing the affinity with the template, more full-length cDNA can be obtained, and the total RNA can be detected as low as 1 pg. It is especially suitable for reverse transcription of a small number of templates and low-copy genes. In addition, the mismatch rate of HiScript® Reverse Transcriptase is also lower than that of M-MLV (RNase H-) Reverse Transcriptase, which improves the fidelity of cloning.    Tolerate a reaction temperature of 50℃, suitable for RNA templates with secondary structure; The template has strong affinity, and more full-length cDNA can be obtained; Highly sensitive, can detect as little as 10 pg of total RNA   Store at -20℃
9999.00
Article number:
R101-01/02
Number of views:
3179
Keywords:
R101-01/02
47.00
Article number:
R021-01
Wild-type M-MLV (Moloney Murine Leukemia Virus) has the following activities: RNA-dependent DNA polymerase activity; DNA-dependent DNA polymerase activity; RNase H activity. Because RNase H activity can catalyze the degradation of RNA in the DNA/RNA hybrid strand, the template RNA may be degraded in the cDNA one-strand synthesis reaction. M-MLV (H-) Reverse Transcriptase is an M-MLV mutant with loss of RNase H activity obtained by site-directed mutagenesis. Compared with the common mutants obtained by deleting the RNase H domain, this product retains the complete protein structure, so it has the same polymerase activity as the wild type, and can be used for longer cDNA synthesis and full-length cDNA library Construction, etc. It also has strand displacement activity and can be used in experiments such as 5'RACE.   Point mutations eliminate RNase H activity to obtain long cDNA products; Stable and reliable reverse transcription performance for RNA templates above 100 ng; Synthetic fragment ≤5 kb; Can be used for experiments such as 5’-RACE reaction and cDNA library construction   Store at -20℃
47.00
Article number:
R021-01
Number of views:
3282
Keywords:
R021-01

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