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Molecular Cloning/Mutagenesis

NGS Library Prep kits

Molecular Research

Drug Discovery

Molecular Cloning/Mutagenesis

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Article number:
RM201-01/02
0.00
Article number:
RM201-01/02
Number of views:
1000
Keywords:
143.00
Article number:
C311
5minTM Universal Ligation Mix is ​​a ready-to-use 2× premix that mixes enzyme and Buffer. It contains T4 DNA ligase, which catalyzes the formation of adjacent 5´-phosphate ends and 3´-hydroxyl ends on double-stranded DNA or RNA to form phosphate diphosphates. Ester bond. This kit is not only suitable for sticky end ligation, but also compatible with DNA ligation with blunt ends and single-base overhanging ends. The optimized connection enhancer and reaction buffer make the reaction more efficient and convenient. The reaction can be quickly connected at 25°C for 5 minutes. The ligation product can directly transform a variety of chemically competent cells.   Compatible: compatible with sticky ends, smooth ends, TA connection, Linker or Adapter connection; Fast: 25℃, 5 minutes can be connected quickly; High efficiency: High cloning efficiency.    Store at -30 ~ -15°C
143.00
Article number:
C311
Number of views:
3859
Keywords:
5min
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ends
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ligation
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dna
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reaction
139.00
Article number:
C601-01/02
This product is the second-generation TOPO cloning kit, which contains the second-generation Topoisomerase, a vector containing the suicide gene ccdB, and a blunt-end factor. Using the second-generation Topoisomerase, with the most suitable buffer, it has higher activity and further improves the cloning efficiency. The efficient connection is completed in 5 minutes at room temperature. Using a vector containing the suicide gene ccdB, when the insert is successfully connected to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thus achieving a "Zero" background. The addition of blunt-end factor makes this product compatible with TA cloning and blunt-end cloning.   Compatible: Compatible with TA cloning and blunt end cloning at the same time; Fast: add fragments and react for 5 minutes; High efficiency: high cloning efficiency and positive rate>95%.    Store at -30~-15°C
139.00
Article number:
C601-01/02
Number of views:
9188
Keywords:
C601-01/02
116.00
Article number:
C115-01/02
ClonExpress® Ultra One Step Cloning Kit is a new generation of recombinant cloning kit, which can quickly and efficiently clone 1-5 fragments to any position of any vector. The highly optimized 2×ClonExpress Mix, 50℃ reaction 5-15 Min completes the reorganization, and the operation is simple and quick.   Fast: 5-15min rapid reorganization; Simple: One-tube Mix, easy to operate; High efficiency: Highly efficient cloning of 50 bp~10 kb fragments, with a positive rate of >95%; Compatible: single fragment, multi-fragment, entry clone three in one; Zero background: provide zero background, ampicillin/canal double resistance carrier   Store at -20°C
116.00
Article number:
C115-01/02
Number of views:
25124
Keywords:
C115-01/02
75.40
Article number:
C113-01/02
ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Linearize the vector and place the insert 5'in the forward/reverse PCR primer Introduce the end sequence of the linearized vector into the end, so that the 5'and 3'ends of the PCR product have the same sequence as the two ends of the linearized vector (15 bp-20 bp). This PCR product and the linearized vector are in a certain ratio After mixing, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning. As a new generation recombinant cloning kit, ClonExpress® II has a unique non-ligase dependent system, which greatly reduces the background of vector self-ligation, and the positive rate can reach more than 95%. The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities, making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification, greatly simplifying The experimental steps.    Simple, fast, efficient, suitable for any carrier; No need to consider the restriction site carried by the insert; Efficient cloning of 50 bp-10 kb DNA fragments; Linearized cloning vector and PCR products can be cloned directly without purification; No ligase, no self-connection, positive rate >95%   Store at  -20°C
75.40
Article number:
C113-01/02
Number of views:
19967
Keywords:
C113-01/02
87.00
Article number:
C112-01/02
ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Linearize the vector and place the insert 5'in the forward/reverse PCR primer Introduce the end sequence of the linearized vector into the end, so that the 5'and 3'ends of the PCR product have the same sequence as the two ends of the linearized vector (15 bp-20 bp). This PCR product and the linearized vector are in a certain ratio After mixing, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning. As a new generation recombinant cloning kit, ClonExpress® II has a unique non-ligase dependent system, which greatly reduces the background of vector self-ligation, and the positive rate can reach more than 95%. The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities, making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification, greatly simplifying The experimental steps.    Simple, fast, efficient, suitable for any carrier; No need to consider the restriction site carried by the insert; Efficient cloning of 50 bp-10 kb DNA fragments; Linearized cloning vector and PCR products can be cloned directly without purification; No ligase, no self-connection, positive rate >95%   Store at  -20°C
87.00
Article number:
C112-01/02
Number of views:
41347
Keywords:
C112-01/02
186.00
Article number:
C215-01/02
Mut Express® MultiS Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, three to five discrete sites on the target plasmid can be introduced at one time. The kit consists of two The ultra-high fidelity of Phanta® Max significantly reduces the possibility of introducing new mutations during the amplification process. Its excellent long-fragment amplification capability is widely applicable to the amplification of any plasmid with a length less than 20 kb. ClonExpress® rapid cloning System utilizes efficient homologous recombination The reaction replaces the traditional annealing reaction. Therefore, use Mut Express® MultiS site-directed mutagenesis kit for DNA site-directed In the case of mutation, the primer design is more flexible, and the amplification reaction is no longer performed in a linear manner, and the template usage is extremely low, which is beneficial to the original Complete degradation of the basic template. The kit is equipped with Exnase® MultiS, a recombinase optimized for multi-base site-directed mutagenesis, And if the amplified product is specific, the DpnI digested product can be used directly in the recombination reaction without DNA purification. Highly optimized reaction buffer Mut Express® MuiltiS Fast Mutagenesis Kit V2 has become the preferred kit for DNA multipoint mutations.    Phanta® Max Super-Fidelity DNA Polymerase provides high-fidelity PCR with the lowest mutation rate; Phanta® Max Super-Fidelity DNA Polymerase has excellent long fragment amplification ability, which is widely applicable to the amplification of any plasmid within 20 kb; The amplification is carried out in an exponential manner, and the template usage is extremely low, which is conducive to the complete degradation of the original methylated template; ClonExpress® rapid cloning system efficiently circularizes PCR products; DpnI eliminates original template contamination Site-directed mutagenesis of three to five discrete sites (more than 50 bp apart) on the target plasmid can be achieved in one step   -Store at 20℃, the shelf life is 1 year
186.00
Article number:
C215-01/02
Number of views:
7161
Keywords:
C215-01/02
81.00
Article number:
C214-01/02
Mut Express® II Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, the target plasmid amplification product is digested with DpnI, ClonExpress® recombination and circularized, and then directly transformed to complete the site-directed mutation. The kit consists of Phanta® Max Super-Fidelity DNA Polymerase amplification module and ClonExpress® rapid cloning module. The ultra-high fidelity of Phanta® MaxSuper-Fidelity DNA Polymerase significantly reduces the possibility of introducing new mutations during the amplification process. The ClonExpress® rapid cloning system uses efficient homologous recombination to replace the traditional annealing loop reaction. Highly optimized reaction buffers, fast operating procedures and extremely high success rate make Mut Express® II Fast Mutagenesis Kit V2 the first choice for DNA site-directed mutagenesis.   Phanta® Max Super-Fidelity DNA Polymerase high-fidelity enzyme provides PCR reactions with ultra-high fidelity; The amplification is carried out in an exponential manner, and the template usage is extremely low, which is conducive to the complete degradation of the original methylated template; The amplified product can be directly used in the recombination reaction after being digested by DpnI; Site-directed mutagenesis can be performed on a single site or two discrete sites (more than 50 bp apart) on the target plasmid at the same time   Store at -20°C
81.00
Article number:
C214-01/02
Number of views:
10089
Keywords:
C214-01/02
9999.00
Article number:
C505-02/03
Fast-T1 is currently the fastest growing competent cell. Outer membrane receptor mutation (tonA) confers resistance to lytic phage T1 and T5 in competent cells; deletion of endonuclease (endA) increases the yield and quality of plasmid DNA; recombinase deficiency (recA) reduces inserts The probability of homologous recombination ensures the stability of inserted DNA; lacZΔM15 makes Fast-T1 competent cells available for blue-white screening. The company’s Fast-T1 competent cells are prepared by a special process, and the pUC19 plasmid is used to detect the transformation efficiency ≥108 cfu/μg DNA.   Fast growth: clones can be seen after 8-9 hours of culture on ampicillin-resistant plates; single clones cultured overnight in 2 ml of LB liquid medium can be cultured for 4-5 hours to extract a small amount of plasmid DNA; Compatible resistance: T1 and T5 phage resistance; Simple operation: for ampicillin-resistant plasmids and ligation products, rapid transformation can be completed in 5 minutes   Store at -70°C (do not store in -20°C or liquid nitrogen!)
9999.00
Article number:
C505-02/03
Number of views:
3843
Keywords:
C505-02/03
9999.00
Article number:
C504-02/03
This product is a chemically transformed super competent cell prepared using E. coli BL21 (DE3) strain, with a transformation efficiency of over 107 cfu/μg. Suitable for expression vectors of T7 promoter system (such as pET series), genotype F-ompThsdS(rB-mB-) galdcm (DE3).   The genetic background is clear; BL21 (DE3) strain, a high-efficiency protein expression host with T7 RNA polymerase as the expression system; Suitable for non-toxic protein recombinant expression   Store at -80°C (do not store in -20°C or liquid nitrogen!)
9999.00
Article number:
C504-02/03
Number of views:
3484
Keywords:
C504-02/03
9999.00
Article number:
C503-02/03
This product is prepared from chemically competent cells for cloning using a modified Inoue method, which enhances the transformation efficiency of large fragments of DNA and greatly increases the growth rate of host bacterial cells. The transformation efficiency exceeds 108 cfu/μg, which is widely used in plasmid transformation, gene cloning, blue-white spot screening and other purposes. Improved Escherichia coli enhances the transformation efficiency of large fragments of DNA and greatly increases the growth rate of host bacteria cells. Genotype Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lacTetr Hte [F′ proAB lacI qZΔM15 Tn10(Tet r) Amy Camr]   XL10 strain, resistant to tetracycline (Tetr) and chloramphenicol (Camr), a new cloned strain; Suitable for efficient conversion of PCR products, cDNA and other sources of unmethylated DNA; EndA1, RecA1 mutations ensure the stability of foreign DNA in the host bacteria and facilitate the extraction of high-quality plasmid DNA; Hte genotype, enhances the transformation efficiency of large fragments of DNA, and greatly increases the growth rate of host bacterial cells; No foreign plasmid DNA residue, transformation efficiency ≥108 cfu/μg pUC19   Store at -80°C (do not store in -20°C or liquid nitrogen!)
9999.00
Article number:
C503-02/03
Number of views:
2640
Keywords:
C503-02/03
9999.00
Article number:
C502-02/03
9999.00
Article number:
C502-02/03
Number of views:
6212
Keywords:
C502-02/03
17.40
Article number:
C301-01
T4 DNA Ligase can catalyze the formation of phosphodiester bonds between the 5'phosphate end and 3'hydroxyl end of the adjacent nucleic acid at the blunt or sticky end of dsDNA. It can also catalyze the connection between RNA and ssDNA or RNA strands in the double strand, but it cannot catalyze the entire single Chain nucleotide connection. Suitable for nucleic acid operations such as labeling RNA 3'-end, circularizing RNA and DNA oligonucleotides and cloning cDNA.   Purity is greater than 99%, no exogenous nuclease activity remains; The classic buffer composition allows the ligation reaction to proceed efficiently   Store at- 20°C
17.40
Article number:
C301-01
Number of views:
4596
Keywords:
C301-01
C301

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