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NGS Library Prep kits

Molecular Research

Drug Discovery

Epigenetics

9999.00
Article number:
S701-01/02
 Hyperactive pA-MNase for CUT&RUN is the fusion of Protein A and engineered ultra-highly active MNase to form a new fusion enzyme with both MNase exonuclease activity and Protein A activity, which is specifically suitable for protein-genome interaction research CUT&RUN technology. Compared with the traditional protein-genome interaction research method ChIP-Seq, CUT&RUN has significant advantages. This technology has a short experimental cycle, high signal-to-noise ratio, good repeatability, and low cell input. It is especially suitable for early embryo development, Research fields such as stem cells, tumors and epigenetics.   (1) Simple and fast workflow: DNA denaturation and bisulfite conversion are completed in one step, and the conversion reaction time is only 140 min (2) High yield: 10 pg-100 ng genomic DNA starting amount, recovery efficiency ≥ 80%, conversion efficiency of unmethylated cytosine ≥ 99% (3) Suitable for various downstream applications: Bisulfite-converted DNA can be used for stable PCR amplification for downstream analysis of NGS sequencing, etc. (4) Compatible with DNA templates from different sources: DNA extracted from cells or tissues of animals, plants, microorganisms; cfDNA; purified PCR products, etc.   Store at -30 ~ -15℃, and transport at -20 ~ 0℃.    
9999.00
Article number:
S701-01/02
Number of views:
1895
Keywords:
cut&run
and
for
dna
is
of
the
research
conversion
958.00
Article number:
S602-01/02
 Hyperactive pG-Tn5 Transposase for CUT&Tag is a fusion of Protein G and engineered ultra-highly active Tn5 transposase to form a new fusion enzyme with both transposase and Protein G activities. It is specially suitable for protein-genome interactions. Researched CUT&Tag technology. Compared with the traditional protein-genome interaction research method ChIP-Seq, CUT&Tag has significant advantages. This technology has a short experimental period, high signal-to-noise ratio, good repeatability, and low cell input. It is especially suitable for early embryo development, Research fields such as stem cells, tumors and epigenetics.   Simple operation: DNA fragmentation and adaptor ligation can be realized in one step, and the transformation from cell to next-generation sequencing library can be realized in only 9 hours Super high activity: both Protein A&Tn5 activity, extremely high DNA fragmentation activity Low initial cell volume: compatible with as few as 60 cells, even single cells High purity: high protein purity, low nucleic acid residue   The entire kit is stored at -85 ~ -65°C and shipped on dry ice; Hyperactive pG-Tn5 Transposase can be stored at -85 ~ -65°C after receipt, and the remaining components can be stored at -30 ~ -15°C; the resulting transposition The seeds are stored at -30 ~ -15℃, and they are valid for one year.    
958.00
Article number:
S602-01/02
Number of views:
3361
Keywords:
cut&tag
and
the
high
protein
is
at
be
stored
transposase
57.00
Article number:
EM202-01/02/03
This product contains EpiArtTM HS Taq DNA Polymerase, dNTP and optimized buffer system, which is suitable for the PCR reaction of bisulfite conversion processing DNA. Compared with ordinary DNA, the DNA damage after bisulfite conversion is serious, which will affect the PCR reaction performance. This product uses a hot-start EpiArtTM HS Taq DNA Polymerase modified by enzyme engineering and an optimized buffer system. Compared with ordinary PCR reagents, this product has better amplification efficiency, sensitivity and specificity for the bisulfite-converted DNA . The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification when used in PCR reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. EM202 contains blue dye, which can be directly used for electrophoresis after the reaction, which is convenient to use. This product can be used with EpiArtTM DNA Methylation Bisulfite Kit (Vazyme #EM101). The PCR product has A at the 3'end, which can be directly cloned into the T vector. It is suitable for ClonExpress® rapid cloning kit (Vazyme #C112/C113/C115) ) And 5 min TA/Blunt-Zero Cloning Kit (Vazyme #C601).   (1) Optimized buffer system, suitable for PCR reaction of bisulfite conversion processing DNA (2) Compared with ordinary PCR reagents, it has better amplification efficiency, sensitivity and specificity for DNA after bisulfite conversion (3) EM202 contains blue dye, which can be directly electrophoresed after the reaction, which is convenient to use   Store at -30 ~ -15℃, transport at -20 ~ 0℃    
57.00
Article number:
EM202-01/02/03
Number of views:
1684
Keywords:
epiart
the
dna
which
and
pcr
for
is
bisulfite
product
57.00
Article number:
EM201-01/02/03
This product contains EpiArtTM HS Taq DNA Polymerase, dNTP and optimized buffer system, which is suitable for the PCR reaction of bisulfite conversion processing DNA. Compared with ordinary DNA, the DNA damage after bisulfite conversion is serious, which will affect the PCR reaction performance. This product uses a hot-start EpiArtTM HS Taq DNA Polymerase modified by enzyme engineering and an optimized buffer system. Compared with ordinary PCR reagents, this product has better amplification efficiency, sensitivity and specificity for the bisulfite-converted DNA . The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification when used in PCR reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. EM202 contains blue dye, which can be directly used for electrophoresis after the reaction, which is convenient to use. This product can be used with EpiArtTM DNA Methylation Bisulfite Kit (Vazyme #EM101). The PCR product has A at the 3'end, which can be directly cloned into the T vector. It is suitable for ClonExpress® rapid cloning kit (Vazyme #C112/C113/C115) ) And 5 min TA/Blunt-Zero Cloning Kit (Vazyme #C601).   (1) Optimized buffer system, suitable for PCR reaction of bisulfite conversion processing DNA (2) Compared with ordinary PCR reagents, it has better amplification efficiency, sensitivity and specificity for DNA after bisulfite conversion (3) EM202 contains blue dye, which can be directly electrophoresed after the reaction, which is convenient to use    Store at -30 ~ -15℃, transport at -20 ~ 0℃
57.00
Article number:
EM201-01/02/03
Number of views:
1510
Keywords:
epiart
the
dna
which
and
pcr
for
is
bisulfite
product
177.00
Article number:
EM101-01/02
DNA methylation is closely related to gene expression and gene function. It plays an important role in physiological and pathological processes such as genetic imprinting, embryonic development, chromosome gene silencing, and cell cycle regulation. Efficient and accurate detection of DNA methylation is important for biological Research in many aspects such as science, genetics, pathology, pharmacology, medical diagnosis, etc. has become more and more important. The most commonly used method to detect DNA methylation is the bisulfite conversion method. The principle of this technology is to use bisulfite Salt treatment of DNA converts unmethylated cytosine into uracil, while methylated cytosine remains unchanged during the conversion process. After conversion, the methylation of DNA can be detected by PCR amplification or DNA sequencing determination. This kit uses thermal denaturation to replace the previous chemical denaturation, combining DNA denaturation and bisulfite conversion into one step, and the conversion reaction time only takes 140 minutes; using membrane desulphonation technology to expand the DNA input range to 100 pg-2 μg; recovery efficiency ≥ 80%, conversion efficiency of unmethylated cytosine ≥ 99%. The transformed product is suitable for downstream applications such as PCR amplification and NGS sequencing.   (1) Optimized buffer system, suitable for PCR reaction of bisulfite conversion processing DNA (2) Compared with ordinary PCR reagents, it has better amplification efficiency, sensitivity and specificity for DNA after bisulfite conversion (3) EM202 contains blue dye, which can be directly electrophoresed after the reaction, which is convenient to use   Store at 15-20℃. Transport at room temperature. The dissolved CT Conversion Mix can be stored at room temperature (15 ~ 25℃) for 24 hours, and frozen (-30 ~ -15℃) for 1 month; Note that it needs to be stored away from light.  
177.00
Article number:
EM101-01/02
Number of views:
2489
Keywords:
epiart
the
dna
and
conversion
to
is
for
of
bisulfite
958.00
Article number:
S603-01/02
958.00
Article number:
S603-01/02
Number of views:
3361
Keywords:

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这是描述信息

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