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Drug Discovery

NGS Library Prep kits

Molecular Research

Drug Discovery

Drug Discovery

506.50
Article number:
DD2103-01
506.50
Article number:
DD2103-01
Number of views:
1000
Keywords:
430.50
Article number:
DD2102-01/02
Add&ReadTM Human Fc Kit can be used to detect the concentration of Human IgG or hFc-fusion protein (hFc-fusion protien) in the cell supernatant or after purification. There is an antibody in the kit that recognizes the Fc region of Human IgG and marks the fluorescent donor Eu (anti-human IgG-Eu); and Human IgG, labeled with fluorescent receptor A2 (Human IgG-A2). In the solution, anti-human IgG-Eu binds to Human IgG-A2, 320 nm light excites fluorescence donor Eu, Eu donor emits 620 nm light, this 620 nm light excites fluorescent acceptor A2, A2 acceptor It emits 665 nm light, which is the phenomenon of fluorescence resonance energy transfer (FRET). When the human IgG or hFc-fusion protien to be tested is added to the solution, it will compete to bind anti-human IgG-Eu and destroy the FRET phenomenon. The concentration of Human IgG or hFc-fusion protien to be tested is inversely proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG, hFc-fusion protein or bispecific antibodies.   Simple steps Has good compatibility Quantitatively accurate   Storage temperature: -30 ~ -15℃, can be transported at -20 ~ 0℃. Diluent and Detection buffer are frozen and transported, and can be stored at 2 ~ 8℃ after use; Anti-human IgG-Eu and Human IgG-A2: It is recommended to store in -20℃ or -70℃ in aliquots to avoid repeated freezing and thawing; IgG Standard: After adding ddH2O to dissolve, it is recommended to aliquot into 1.5 ml EP tubes and store at -20°C or -70°C to avoid repeated freezing and thawing.  
430.50
Article number:
DD2102-01/02
Number of views:
2032
Keywords:
kit
the
to
and
human
igg
or
is
be
nm
430.50
Article number:
DD2101-01/02
Add&ReadTM Human IgG Kit can be used to detect the concentration of Human IgG (hlgG) in the cell supernatant or after purification. There are two antibodies that recognize hIgG in the kit, namely: the antibody that recognizes the Fc region, anti-hFc (labeled fluorescent donor) Eu, anti-hFc-Eu); antibody anti-hFab (labeled fluorescent receptor A2, anti-hFab-A2) that recognizes the Fab region. When anti-hFc-Eu and anti-hFab-A2 are close (combination occurs), fluorescence resonance energy transfer (FRET) can occur. Use 320 nm light to excite the fluorescence donor Eu, Eu emits 620 nm light, this 620 nm light excites the fluorescent acceptor A2, and A2 emits 665 nm light. The concentration of Human IgG to be tested is directly proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG.   Simple steps Has good compatibility Quantitatively accurate   Storage temperature: -30 ~ -15℃, can be transported at -20 ~ 0℃. Diluent and Detection buffer are frozen and transported, and can be stored at 2 ~ 8℃ after use; Anti-hFc-Eu and Anti-hFab-A2: It is recommended to store in -20℃ or -70℃ in aliquots to avoid repeated freezing and thawing; IgG Standard: After adding ddH2O to dissolve, it is recommended to aliquot into 1.5 ml EP tubes and store at -20°C or -70°C to avoid repeated freezing and thawing.  
430.50
Article number:
DD2101-01/02
Number of views:
1646
Keywords:
kit
the
to
and
nm
be
can
igg
in
at
9999.00
Article number:
DD1204-01/02/03/04
Bright-LiteTM Luciferase Assay system is an ultra-sensitive, stable and homogeneous firefly luciferase reporter gene detection kit. This kit contains high-purity Luciferin and optimized reaction reagents, which makes the reaction more stable, more resistant to the environment, and less odor. Directly add the mixed Bright-LiteTM detection reagent to the cell culture to lyse the cells, release luciferase, and then generate a reaction as shown in the figure and send out a stable light signal.   Long-term storage: -30 ~ -15℃, transportation conditions: ≤0℃. Before mixing: Bright-Lite Luciferase Assay Buffer can be stored at room temperature (< 25℃) or 2 ~ 8℃ for a long time; Bright-Lite Luciferase Assay Substrate can be stored at 2 ~ 8℃ for 30 days. After mixing: Bright-LiteTM detection reagent can be stored at room temperature for 1 day (> 80% activity) or at 2 ~ 8 ℃ for 1 day (> 90% activity). It can be kept stable after 10 cycles of repeated freezing and thawing. Unused reagents can be stored at -20°C for 14 days (> 90% activity). If it is not used for a long time, it is recommended to store at -70°C.  
9999.00
Article number:
DD1204-01/02/03/04
Number of views:
1217
Keywords:
bright-lite
the
and
luciferase
to
is
reaction
stable
it
at
9999.00
Article number:
DD1203-01/02/03/04
One-LiteTM Luciferase Assay system is a highly sensitive, stable and homogeneous firefly luciferase reporter gene detection kit. This kit contains high-purity Luciferin and optimized reaction reagents, which makes the reaction more stable, more resistant to the environment, and less odor. Directly add the mixed One-LiteTM detection reagent to the cell culture to lyse the cells and release the luciferase, then the reaction as shown in the figure can be generated and a stable light signal can be emitted.   Long-term storage: -30 ~ -15℃, transportation conditions: ≤0℃. Before mixing: One-Lite Luciferase Assay Buffer can be stored at room temperature for 90 days (> 90% activity) or long-term storage at 2 ~ 8℃; One-Lite Luciferase Assay Substrate can be stored at room temperature for 21 days or at 2 ~ 8 ℃ for 90 days (> 85% activity). After mixing: One-LiteTM detection reagent can be stored at room temperature for 1 day (> 80% activity) or at 2 ~ 8 ℃ for 5 days (> 85% activity). It can be kept stable even after 10 cycles of repeated freezing and thawing. Unused reagents can be stored at -20°C for 60 days. If not used for a long time, it is recommended to store at -70°C.  
9999.00
Article number:
DD1203-01/02/03/04
Number of views:
1158
Keywords:
one-lite
at
the
be
for
can
and
luciferase
activity
stored
9999.00
Article number:
DD1202-01/02/03/04
Bright-LiteTM Luciferase Assay system is an ultra-sensitive, stable and homogeneous firefly luciferase reporter gene detection kit. This kit contains high-purity Luciferin and optimized reaction reagents, which makes the reaction more stable, more resistant to the environment, and less odor. Directly add the mixed Bright-LiteTM detection reagent to the cell culture to lyse the cells, release luciferase, and then generate a reaction as shown in the figure and send out a stable light signal.   Long-term storage: -30 ~ -15℃, transportation conditions: ≤0℃. Before mixing: Bright-Lite Luciferase Assay Buffer can be stored at room temperature (< 25℃) or 2 ~ 8℃ for a long time; Bright-Lite Luciferase Assay Substrate can be stored at 2 ~ 8℃ for 30 days. After mixing: Bright-LiteTM detection reagent can be stored at room temperature for 1 day (> 80% activity) or at 2 ~ 8 ℃ for 1 day (> 90% activity). It can be kept stable after 10 cycles of repeated freezing and thawing. Unused reagents can be stored at -20°C for 14 days (> 90% activity). If it is not used for a long time, it is recommended to store at -70°C.  
9999.00
Article number:
DD1202-01/02/03/04
Number of views:
1116
Keywords:
system
the
and
luciferase
to
is
reaction
stable
it
at
9999.00
Article number:
DD1102-01/02/03
CellCounting-Lite® 3D is a cell viability detection reagent based on luciferase system. This reagent contains high-purity luciferin (Luciferin), thermostable luciferase (Luciferase), and optimized reaction reagents, which makes the lysis ability stronger and is more suitable for micro-tissue cell cluster samples under 3D cell culture conditions . Add this product to the cell culture to lyse the cell mass and release ATP, which can produce a reaction as shown in the figure and send out a stable light signal. The luminescence intensity is proportional to the amount of ATP, that is, the number of living cells within a certain range, so this product can quantitatively detect the number of living cells.   Operation is simpler: single component, single step Daily use is more convenient: no need to pack freeze-thaw Stronger cracking capacity: accurate quantification   Long-term storage: -30 ~ -15℃, transportation conditions: ≤0℃. After melting, CellCounting-Lite® 3D can be stored at room temperature for 7 days or at 2 ~ 8℃ for 60 days (>85% activity). This product can be kept stable after 10 cycles of repeated freezing and thawing. If the unused reagent is not used for a long time, it is recommended to store it at -20℃.  
9999.00
Article number:
DD1102-01/02/03
Number of views:
1196
Keywords:
3d
the
is
cell
to
and
this
for
can
of
9999.00
Article number:
DD1201-01/02/03
Bio-LiteTM is a highly sensitive, stable and homogeneous firefly luciferase reporter gene detection kit. This kit contains high-purity luciferin (Luciferin) and optimized reaction reagents, which makes the reaction more stable, more resistant to the environment, and less odor. Directly add the mixed Bio-LiteTM detection reagent to the cell culture to lyse the cells and release the luciferase, which can produce the reaction as shown in the figure and send out a stable light signal.   Long-term storage: -30 ~ -15℃, can be transported at -20 ~ 0℃. Before mixing: Bio-Lite Luciferase Assay Buffer can be stored at room temperature for 90 days (> 90% activity) or long-term storage at 2 ~ 8℃; Bio-Lite Luciferase Assay Substrate can be stored at room temperature for 21 days or at 2 ~ 8°C for 90 days (> 85% activity). After mixing: Bio-Lite TM detection reagent can be stored at room temperature for 1 day (> 80% activity) or at 2 ~ 8 ℃ for 5 days (> 85% activity). It can be kept stable even after 10 cycles of repeated freezing and thawing. Unused reagents can be stored at -20°C for 60 days. If not used for a long time, it is recommended to store at -70°C.  
9999.00
Article number:
DD1201-01/02/03
Number of views:
1675
Keywords:
system
at
the
for
can
be
and
activity
stored
days
9999.00
Article number:
DD1101-01/02/03
CellCounting-LiteTM 2.0 is a cell viability detection reagent based on luciferase system. The reagent contains high purity luciferin and thermostable luciferase. Add this product to the cell culture to lyse the cells and release ATP, then the reaction as shown in the figure can be generated and a stable "glow" type signal will be sent out. The luminescence intensity is proportional to the amount of ATP, that is, the number of living cells within a certain range, so this product can quantitatively detect the number of living cells.   The operation is simpler: single component, single step.Daily use is more convenient: no need to pack freeze-thaw Can achieve seamless switching: the basic performance is consistent with P company products Long-term storage: -30 ~ -15℃, can be transported at -20 ~ 0℃. After melting, CellCounting-Lite TM 2.0 can be stored at room temperature for 7 days or at 2-8°C for 60 days (> 85% activity). This product can be kept stable for 5 cycles of repeated freezing and thawing. If the unused reagent is not used for a long time, it is recommended to store it at -20℃.  
9999.00
Article number:
DD1101-01/02/03
Number of views:
1656
Keywords:
2.0
the
is
can
be
to
at
for
and
of
9999.00
Article number:
A213-01/02
In normal cells, phosphatidylserine (PS) is only distributed inside the lipid bilayer of the cell membrane. In the early stage of apoptosis, the PS in the cell membrane turns from the inside to the outside, exposing the PS to the external environment of the cell. 。Annexin V is a 35-36 kDa Ca2+-dependent phospholipid binding protein with high affinity to PS, so it can bind to the cell membrane of cells in the early stage of apoptosis through the PS exposed outside the cell. Therefore, Annexin V is recognized as an early detection cell One of the sensitive indicators of apoptosis. The Annexin V is labeled with Phycoerythrin (PE). The labeled Annexin V retains a high affinity for PS and can be used as a probe to detect apoptosis using flow cytometry. Since PS valgus occurs in the early stage of apoptosis, Annexin V-PE staining can identify the occurrence of apoptosis in the early stage of apoptosis. 7-Aminoactinomycin (7-AAD) is a nucleic acid dye. 7-AAD cannot penetrate the complete cell membrane of normal cells or early apoptotic cells, but can penetrate membrane-damaged cells such as late apoptotic cells or necrotic cells The cell membrane and the DNA within it can be used to distinguish early apoptotic cells from late apoptotic cells or necrotic cells. Therefore, co-staining Annexin V-PE and 7-AAD can distinguish cells in different stages of apoptosis.   Strong specific staining PE dye is red fluorescence, and can be co-dye with green fluorescence   All components of this kit are stored at 2-8°C. Annexin V-PE and 7-AAD Staining Solution should be stored in the dark and should not be frozen.
9999.00
Article number:
A213-01/02
Number of views:
2927
Keywords:
7-aad
the
of
cells
in
ps
annexin
can
early
is

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这是描述信息

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