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NGS Library Prep kits

Molecular Research

Drug Discovery

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799.00
Article number:
NR605
VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina is specially designed for the preparation of transcriptome libraries for Illumina platform. The kit is universal and suitable for RNA library construction of RNA that have been obtained by Poly(A)-based mRNA enrichment or rRNA depletion. The kit contains two types of cDNA 2nd Strand synthesis buffer, which can be chosen for library construction for non-stranded or stranded RNA-Seq transcriptome analysis. This kit combines 2nd Strand cDNA synthesis, end-repair and dA-Tailing into one step, with no need of purification, which greatly simplifies the process of library construction and shortens the operation time. The optimized reaction system improves the library construction efficiency, is compatible with lower-input RNA, and has uniform coverage for different amounts of input-RNA. Libraries of specific sizes, which can be customized, can be obtained after size selection with magnetic beads. All the enzymes and buffer provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability.
799.00
Article number:
NR605
Number of views:
1000
Keywords:
kit
297.00
Article number:
180kDaPrestainedProteinMarkeriscomposedof10kindsprestainedprotein.Themolecularweightrangesfrom10kDato180kDa.Amongthem,70kDaisaredband,15kDa,25kDa,35kDa,40kDa,55kDa,100kDa,130kDa,and180kDaarebluebands,and10kDaisagreenband.Thisproductdynamicallyindicatestheproteinelectrophoresisstatusandthemolecularweightofthetargetproteincanbeclearlyjudged.Besides,itcanbetransferredtoPVDFmembrane(orNCmembrane)afterSDS-PAGEelectrophoresis,andclearcolorbandscanbeseentomonitorWesternBlotTransferefficiency.Therecommendedloadingvolumeforthisproductis5µl.
297.00
Article number:
Number of views:
1000
Keywords:
mp102
kda
the
is
and
protein
10
be
can
180
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Article number:
R513-01/02
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Article number:
R513-01/02
Number of views:
1000
Keywords:
0.00
Article number:
RM301-01/02
0.00
Article number:
RM301-01/02
Number of views:
1000
Keywords:
0.00
Article number:
VNP-32YL
0.00
Article number:
VNP-32YL
Number of views:
1000
Keywords:
0.00
Article number:
CD302-02
0.00
Article number:
CD302-02
Number of views:
1000
Keywords:
0.00
Article number:
C8909C
Anti-SARS-CoV-2 Neutralizing Antibody ELISA Kit Rapid & High-throughput Evaluation of Vaccination •High-throughput •Safe & Stable •Time saving Many studies have proved that SARS-CoV-2 enters target cells by using its RBD to bind with the cell surface receptor ACE2. The neutralizing antibody can bind with RBD of virus and prevent it from binding with ACE2, thus playing a protective role. Anti-SARS-CoV-2 Neutralizing Antibody ELISA Kit can help to check for the presence of protective neutralizing antibody. Order Information Cat. No Specimen Packing Size Shelf Life Storage C8909C Serum/Plasma 96 Tests/Kit 10 Months 2℃- 8℃          
0.00
Article number:
C8909C
Number of views:
1000
Keywords:
0.00
Article number:
RC312-01/02
0.00
Article number:
RC312-01/02
Number of views:
1000
Keywords:
0.00
Article number:
RM102-01/02
0.00
Article number:
RM102-01/02
Number of views:
1000
Keywords:
0.00
Article number:
C6002C;C6003C
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Article number:
C6002C;C6003C
Number of views:
1000
Keywords:
0.00
Article number:
C8602C
0.00
Article number:
C8602C
Number of views:
1000
Keywords:
506.50
Article number:
DD2103-01
506.50
Article number:
DD2103-01
Number of views:
1000
Keywords:
185.00
Article number:
Q222-CN-00
185.00
Article number:
Q222-CN-00
Number of views:
1000
Keywords:
0.00
Article number:
DD3102
0.00
Article number:
DD3102
Number of views:
1000
Keywords:
100.00
Article number:
VAHTS TM HiFi Amplification Mix is a new High-fidelity PCR amplification master mix, which is applicable to high-throughput sequencing library PCR amplification. The master mix is based on VAHTS TM HiFi DNA Polymerase, which is the new generation of DNA polymerase with high yield and fidelity engineered from Pfu DNA Polymerase. The sensitivity of this enzyme has been greatly improved, with extremely high amplification efficiency and extensive adaptability of the template. It greatly improves the amplification output and the platform period.
100.00
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Number of views:
1000
Keywords:
0.00
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0.00
Article number:
Number of views:
1000
Keywords:
0.00
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0.00
Article number:
Number of views:
1000
Keywords:
0.00
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0.00
Article number:
Number of views:
1000
Keywords:
0.00
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VAHTS TM HiFi Amplification Mix is a new High-fidelity PCR amplification master mix, which is applicable to high-throughput sequencing library PCR amplification. The master mix is based on VAHTS TM HiFi DNA Polymerase, which is the new generation of DNA polymerase with high yield and fidelity engineered from Pfu DNA Polymerase. The sensitivity of this enzyme has been greatly improved, with extremely high amplification efficiency and extensive adaptability of the template. It greatly improves the amplification output and the platform period.
0.00
Article number:
Number of views:
1000
Keywords:
165.00
Article number:
N101-01
In the presence of templates and primers, T4 DNA polymerase catalyzes the synthesis of DNA along the 5´→3´ direction. This enzyme also has 3´→5´ exonuclease activity, which is stronger than DNA polymerase I. Unlike DNA polymerase I, T4 DNA polymerase does not have 5´→3´ exonuclease activity.   Connect the sticky end or blunt end quickly in 5 minutes at room temperature; T/A cloning; dsDNA cut repair   Store at -20℃
165.00
Article number:
N101-01
Number of views:
1000
Keywords:
301.00
Article number:
P507-02
301.00
Article number:
P507-02
Number of views:
1000
Keywords:
71.00
Article number:
P507-01
71.00
Article number:
P507-01
Number of views:
1000
Keywords:
0.00
Article number:
N611-02
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Article number:
N611-02
Number of views:
1000
Keywords:
0.00
Article number:
N611-01
0.00
Article number:
N611-01
Number of views:
1000
Keywords:
0.00
Article number:
TD601
0.00
Article number:
TD601
Number of views:
1000
Keywords:
48.00
Article number:
N106-01
48.00
Article number:
N106-01
Number of views:
1000
Keywords:
870.00
Article number:
NA102-01
VAHTSTM AmpSeq Cancer HotSpot Panel contains 207 primer pairs that can be used to amplify hotspot mutation regions of human tumor-related genes. The products cover a total of 50 proto-oncogenes and suppressor genes. 2800 common mutation sites of oncogenes. When this product is used in conjunction with VAHTSTM AmpSeq Library Prep Kit V2 (Vazyme #NA201), 20 μl multiple amplification system needs to add 10 μl of this Panel (that is, the concentration of this Panel is 2×), which can perform highly uniform multiplex amplification on the target area. Amplifier builds library, and provides VAHTSTM AmpSeq Adapters 1-96 for Illumina (Vazyme #NA111) and VAHTSTM AmpSeq Adapters 1-96 for Ion Torrent (Vazyme #NA121) for different sequencing platforms. The premixed primer panel saves researchers the time to design and prepare primers, and is suitable for starting DNA templates as low as 1ng and a variety of complex templates, such as FFPE samples and cfDNA. The fragment size of the amplified product using this product is between 111 bp-187 bp.   Store at -30 ~ -15℃
870.00
Article number:
NA102-01
Number of views:
1000
Keywords:
vahts
the
and
of
is
vahtstm
panel
ampseq
this
for
1200.00
Article number:
HF001-1 
HiscriptHighFidelityOneStepRT-PCRKit一步法高效RT-PCR试剂盒逆转录和PCR在同一管内进行检测灵敏度可达0.1pg总RNA可扩增超过10kb的长片段图1A.以Hela细胞总RNA为模板,用Hiscript® HighFidelityOneStepRT-PCRKit (HF001)扩增555bpGAPDH片段。检测灵敏度可达到0.1pg。B.以猪蓝耳病毒PRRSVRNA为模板,用Hiscript®HighFidelityOneStepRT-PCRKit(HF001)扩增660bp片段。检测灵敏度可达到0.2TCLD50。C.以1μgHela细胞总RNA为模板,用Hiscript®HighFidelityOneStepRT-PCRKit(HF001)扩增不同长度的片段。可成功扩增长达12.1kb的UTRN片段。本试剂盒专为以RNA为模板(如RNA病毒)的终点法PCR检测而设计。使用基因特异引物(GSP),逆转录和PCR反应在一管内完成,不需要额外的开管/移液操作,大大提高了检测通量,并降低了污染的风险。整合HiScript®IIReverseTranscriptase以及ChampagneTaqTMDNAPolymerase的优越性能,配合经过优化的缓冲体系,Hiscript®HighFidelityOneStepRT-PCRKit(HF001) 可扩增片段长达10kb以上。试剂盒以便捷的MasterMix形式提供。2×OneStepMix包含优化的缓冲体系和dNTPs;OneStepEnzymeMix包含比例优化的HiScript®IIReverseTranscriptase、RNaseinhibitor以及ChampagneTaqTM plusDNAPolymerase,操作简单快捷。组分HF001-0150rxn(50ul/rxn)RNasefreeddH2O1ml×22×OneStepMixa625μl×2OneStepEnzymeMixb125μl10×Loadingbuffer1.25mla.包含dNTP和缓冲体系b.包含HiScript® IIReverseTranscriptase、RNaseinhibitor以及ChampagneTaqTM plusDNAPolymerase-30~-15℃保存。
1200.00
Article number:
HF001-1 
Number of views:
4965
Keywords:
fidelity
one
step
hiscript
片段
rt-pcr
扩增
rna
kit
检测
HF001-01
HF001
hf001
9999.00
Article number:
DC112-01/02
This kit is mainly suitable for extracting genomic DNA from a variety of fresh or frozen anticoagulant blood, cells, animal tissues and bacterial samples. The kit uses silica gel membrane purification technology, no need to use toxic reagents such as phenol, chloroform, and time-consuming alcohol precipitation during the extraction process, and to maximize the removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as restriction digestion, PCR, and Southern hybridization.   High quality and good integrity: The extracted DNA has the characteristics of high yield, good purity, and complete fragments. Wide range of samples: suitable for direct extraction of DNA from a variety of fresh or frozen anticoagulated blood, cells, animal tissues and bacterial samples. Simple and fast: Animal tissue provides a 30min quick extraction plan.     Proteinase K is stored at 2-8°C, and other components are stored at room temperature (15-25°C).
9999.00
Article number:
DC112-01/02
Number of views:
1458
Keywords:
bacteria
and
the
of
dna
extraction
animal
high
for
extracted
115.00
Article number:
R504-01
RNase and nucleic acid scavenger, a non-toxic spray reagent, used to remove RNase contamination on the surface of instruments, equipment, and experimental desktops. At the same time, it can establish an RNA-related experimental operating environment for nucleic acid templates without affecting the stability of RNA Sex.   High removal efficiency: can completely remove nucleic acid templates such as RNase and DNA/RNA in the experimental environment  Safe, non-toxic, easy to use: liquid, spray and use No damage to equipment: non-corrosive, can be sprayed directly on the surface of the instrument   Store at room temperature (15 ~ 25℃)
115.00
Article number:
R504-01
Number of views:
1286
Keywords:
rna
the
and
experimental
acid
rnase
can
of
nucleic
to
430.50
Article number:
DD2102-01/02
Add&ReadTM Human Fc Kit can be used to detect the concentration of Human IgG or hFc-fusion protein (hFc-fusion protien) in the cell supernatant or after purification. There is an antibody in the kit that recognizes the Fc region of Human IgG and marks the fluorescent donor Eu (anti-human IgG-Eu); and Human IgG, labeled with fluorescent receptor A2 (Human IgG-A2). In the solution, anti-human IgG-Eu binds to Human IgG-A2, 320 nm light excites fluorescence donor Eu, Eu donor emits 620 nm light, this 620 nm light excites fluorescent acceptor A2, A2 acceptor It emits 665 nm light, which is the phenomenon of fluorescence resonance energy transfer (FRET). When the human IgG or hFc-fusion protien to be tested is added to the solution, it will compete to bind anti-human IgG-Eu and destroy the FRET phenomenon. The concentration of Human IgG or hFc-fusion protien to be tested is inversely proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG, hFc-fusion protein or bispecific antibodies.   Simple steps Has good compatibility Quantitatively accurate   Storage temperature: -30 ~ -15℃, can be transported at -20 ~ 0℃. Diluent and Detection buffer are frozen and transported, and can be stored at 2 ~ 8℃ after use; Anti-human IgG-Eu and Human IgG-A2: It is recommended to store in -20℃ or -70℃ in aliquots to avoid repeated freezing and thawing; IgG Standard: After adding ddH2O to dissolve, it is recommended to aliquot into 1.5 ml EP tubes and store at -20°C or -70°C to avoid repeated freezing and thawing.  
430.50
Article number:
DD2102-01/02
Number of views:
2032
Keywords:
kit
the
to
and
human
igg
or
is
be
nm
430.50
Article number:
DD2101-01/02
Add&ReadTM Human IgG Kit can be used to detect the concentration of Human IgG (hlgG) in the cell supernatant or after purification. There are two antibodies that recognize hIgG in the kit, namely: the antibody that recognizes the Fc region, anti-hFc (labeled fluorescent donor) Eu, anti-hFc-Eu); antibody anti-hFab (labeled fluorescent receptor A2, anti-hFab-A2) that recognizes the Fab region. When anti-hFc-Eu and anti-hFab-A2 are close (combination occurs), fluorescence resonance energy transfer (FRET) can occur. Use 320 nm light to excite the fluorescence donor Eu, Eu emits 620 nm light, this 620 nm light excites the fluorescent acceptor A2, and A2 emits 665 nm light. The concentration of Human IgG to be tested is directly proportional to the FRET signal value (the ratio of light intensity at 665 nm/620 nm). This kit can be used to detect cell supernatant and purified human IgG.   Simple steps Has good compatibility Quantitatively accurate   Storage temperature: -30 ~ -15℃, can be transported at -20 ~ 0℃. Diluent and Detection buffer are frozen and transported, and can be stored at 2 ~ 8℃ after use; Anti-hFc-Eu and Anti-hFab-A2: It is recommended to store in -20℃ or -70℃ in aliquots to avoid repeated freezing and thawing; IgG Standard: After adding ddH2O to dissolve, it is recommended to aliquot into 1.5 ml EP tubes and store at -20°C or -70°C to avoid repeated freezing and thawing.  
430.50
Article number:
DD2101-01/02
Number of views:
1646
Keywords:
kit
the
to
and
nm
be
can
igg
in
at
9999.00
Article number:
DD1204-01/02/03/04
Bright-LiteTM Luciferase Assay system is an ultra-sensitive, stable and homogeneous firefly luciferase reporter gene detection kit. This kit contains high-purity Luciferin and optimized reaction reagents, which makes the reaction more stable, more resistant to the environment, and less odor. Directly add the mixed Bright-LiteTM detection reagent to the cell culture to lyse the cells, release luciferase, and then generate a reaction as shown in the figure and send out a stable light signal.   Long-term storage: -30 ~ -15℃, transportation conditions: ≤0℃. Before mixing: Bright-Lite Luciferase Assay Buffer can be stored at room temperature (< 25℃) or 2 ~ 8℃ for a long time; Bright-Lite Luciferase Assay Substrate can be stored at 2 ~ 8℃ for 30 days. After mixing: Bright-LiteTM detection reagent can be stored at room temperature for 1 day (> 80% activity) or at 2 ~ 8 ℃ for 1 day (> 90% activity). It can be kept stable after 10 cycles of repeated freezing and thawing. Unused reagents can be stored at -20°C for 14 days (> 90% activity). If it is not used for a long time, it is recommended to store at -70°C.  
9999.00
Article number:
DD1204-01/02/03/04
Number of views:
1217
Keywords:
bright-lite
the
and
luciferase
to
is
reaction
stable
it
at
9999.00
Article number:
DD1203-01/02/03/04
One-LiteTM Luciferase Assay system is a highly sensitive, stable and homogeneous firefly luciferase reporter gene detection kit. This kit contains high-purity Luciferin and optimized reaction reagents, which makes the reaction more stable, more resistant to the environment, and less odor. Directly add the mixed One-LiteTM detection reagent to the cell culture to lyse the cells and release the luciferase, then the reaction as shown in the figure can be generated and a stable light signal can be emitted.   Long-term storage: -30 ~ -15℃, transportation conditions: ≤0℃. Before mixing: One-Lite Luciferase Assay Buffer can be stored at room temperature for 90 days (> 90% activity) or long-term storage at 2 ~ 8℃; One-Lite Luciferase Assay Substrate can be stored at room temperature for 21 days or at 2 ~ 8 ℃ for 90 days (> 85% activity). After mixing: One-LiteTM detection reagent can be stored at room temperature for 1 day (> 80% activity) or at 2 ~ 8 ℃ for 5 days (> 85% activity). It can be kept stable even after 10 cycles of repeated freezing and thawing. Unused reagents can be stored at -20°C for 60 days. If not used for a long time, it is recommended to store at -70°C.  
9999.00
Article number:
DD1203-01/02/03/04
Number of views:
1158
Keywords:
one-lite
at
the
be
for
can
and
luciferase
activity
stored
9999.00
Article number:
DD1202-01/02/03/04
Bright-LiteTM Luciferase Assay system is an ultra-sensitive, stable and homogeneous firefly luciferase reporter gene detection kit. This kit contains high-purity Luciferin and optimized reaction reagents, which makes the reaction more stable, more resistant to the environment, and less odor. Directly add the mixed Bright-LiteTM detection reagent to the cell culture to lyse the cells, release luciferase, and then generate a reaction as shown in the figure and send out a stable light signal.   Long-term storage: -30 ~ -15℃, transportation conditions: ≤0℃. Before mixing: Bright-Lite Luciferase Assay Buffer can be stored at room temperature (< 25℃) or 2 ~ 8℃ for a long time; Bright-Lite Luciferase Assay Substrate can be stored at 2 ~ 8℃ for 30 days. After mixing: Bright-LiteTM detection reagent can be stored at room temperature for 1 day (> 80% activity) or at 2 ~ 8 ℃ for 1 day (> 90% activity). It can be kept stable after 10 cycles of repeated freezing and thawing. Unused reagents can be stored at -20°C for 14 days (> 90% activity). If it is not used for a long time, it is recommended to store at -70°C.  
9999.00
Article number:
DD1202-01/02/03/04
Number of views:
1116
Keywords:
system
the
and
luciferase
to
is
reaction
stable
it
at
9999.00
Article number:
DD1102-01/02/03
CellCounting-Lite® 3D is a cell viability detection reagent based on luciferase system. This reagent contains high-purity luciferin (Luciferin), thermostable luciferase (Luciferase), and optimized reaction reagents, which makes the lysis ability stronger and is more suitable for micro-tissue cell cluster samples under 3D cell culture conditions . Add this product to the cell culture to lyse the cell mass and release ATP, which can produce a reaction as shown in the figure and send out a stable light signal. The luminescence intensity is proportional to the amount of ATP, that is, the number of living cells within a certain range, so this product can quantitatively detect the number of living cells.   Operation is simpler: single component, single step Daily use is more convenient: no need to pack freeze-thaw Stronger cracking capacity: accurate quantification   Long-term storage: -30 ~ -15℃, transportation conditions: ≤0℃. After melting, CellCounting-Lite® 3D can be stored at room temperature for 7 days or at 2 ~ 8℃ for 60 days (>85% activity). This product can be kept stable after 10 cycles of repeated freezing and thawing. If the unused reagent is not used for a long time, it is recommended to store it at -20℃.  
9999.00
Article number:
DD1102-01/02/03
Number of views:
1196
Keywords:
3d
the
is
cell
to
and
this
for
can
of
9999.00
Article number:
QV110 -01/02
Animal Detection U+ Probe Master Mix is ​​a special premix suitable for the detection of African swine fever virus (ASFV) by the probe method. It only needs to add additional primers, probes and templates, which is easy to use. This premix uses an upgraded hot-start Taq enzyme and a carefully optimized Buffer to improve the detection sensitivity of low-concentration templates. This product introduces the dUTP/UDG anti-pollution system, which can function at room temperature to remove the pollution in the system and ensure the accuracy of the results. At the same time, it is compatible with fast programs, reducing detection time.   Excellent amplification sensitivity: The upgraded hot-start Taq DNA polymerase with a carefully optimized buffer system improves the detection sensitivity of low-concentration templates; dUTP/UDG anti-pollution system: Introduce dUTP/Heat-labile UDG anti-pollution system to efficiently degrade aerosol pollution of products, reduce false positives, and ensure that the results are true and reliable; Support fast program: Compatible with fast program, the result detection can be completed within 40 minutes; Excellent storage stability: The reagent has stable performance when stored at 37°C for 9 days, repeated freezing and thawing 30 times, and transported for 4 days.    Store at -30~-15°C
9999.00
Article number:
QV110 -01/02
Number of views:
1583
Keywords:
detection
the
and
system
to
of
at
is
sensitivity
794.00
Article number:
NDM607-01/02
VAHTS® Universal DNA Library Prep Kit for MGI is a library construction kit optimized for the MGI high-throughput sequencing platform. This kit can convert 100 pg-4 μg Input DNA into a library dedicated to MGI high-throughput sequencing platform. As a new upgraded version, VAHTS® Universal DNA Library Prep Kit for MGI greatly improves the library conversion rate and amplified library output through the overall improvement of the end repair module, the connection module and the library amplification module. It is widely applicable to a variety of Construction of PCR library of samples. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.   Efficient repair of DNA damage: effective repair of base damage, nicks, gaps and 3’ end closures High library construction efficiency: both library conversion rate and amplification output are improved Wide template compatibility: compatible with samples such as gDNA, FFPE DNA, cfDNA, Amplicons, etc. Perfect compatibility with different quality samples: DNA repair enzymes have excellent performance on FFPE DNA with different DIN values Short time-consuming library construction: the shortest single library construction only takes 90 minutes      Store at -30 ~ -15℃. Can be transported at -20 ~ 0℃
794.00
Article number:
NDM607-01/02
Number of views:
1668
Keywords:
vahts
library
the
dna
of
and
construction
kit
mgi
repair

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这是描述信息

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