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Phanta EVO HS Super-Fidelity DNA Polymerase

NGS Library Prep kits

Molecular Research

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Number of views:
2562

Phanta EVO HS Super-Fidelity DNA Polymerase

Phanta Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. With the genetic engineering of Pfu DNA Polymerase, the processivity of Phanta Super-Fidelity DNA Polymerase has been greatly improved. Even for very complex templates, the amplification reaction can be completed accurately and quickly. Phanta EVO HS Super-Fidelity DNA Polymerase is an upgraded version of Phanta HS Super-Fidelity DNA Polymerase. Compared with the previous generation product, Phanta EVO HS has added a unique extension factor and deeply optimized the reaction system to further improve its amplification stability, fidelity and ability to amplify long fragments. Using simple templates such as lambda DNA and plasmids, Phanta EVO HS can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta EVO HS can also effectively amplify fragments up to 20 kb. Its amplification mismatch rate is 1/100 of that of ordinary Taq polymerase and 1/12 of Pfu polymerase; and the amplification speed can reach 15 seconds/kb. In addition, Phanta EVO HS has good resistance to PCR inhibitors and can be used for bacteria, fungi, Direct PCR of plant tissue, animal tissue or whole blood samples. Strict fidelity performance and excellent amplification efficiency make Phanta EVO HS the first choice for high-fidelity PCR. Phanta EVO HS has added two monoclonal antibodies that can inhibit its 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling highly specific Hot Start PCR . The enzyme has 5'→3' polymerase activity and 3'→5' exonuclease activity, and the amplified product is blunt-ended, which is suitable for ClonExpress rapid cloning kit (C112/C113). 5 × EVO Buffer has good adaptability to PCR amplification of simple or complex templates, short fragments or long fragments. It already contains 10 mM Mg2+, you can use the MgCl2 or PCR Enhancer provided with the enzyme to optimize the reaction system.    Super fidelity: the fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. Every 300,000 bases amplified, with less than 5 mismatches; Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments; Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of cDNA can reach 10 kb, and the effective amplification length of genome can reach 20 kb; Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.   Store at -20°C
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Number of views:
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Product serial number
P504-d1/d3
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Phanta Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. With the genetic engineering of Pfu DNA Polymerase, the processivity of Phanta Super-Fidelity DNA Polymerase has been greatly improved. Even for very complex templates, the amplification reaction can be completed accurately and quickly.
Phanta EVO HS Super-Fidelity DNA Polymerase is an upgraded version of Phanta HS Super-Fidelity DNA Polymerase. Compared with the previous generation product, Phanta EVO HS has added a unique extension factor and deeply optimized the reaction system to further improve its amplification stability, fidelity and ability to amplify long fragments. Using simple templates such as lambda DNA and plasmids, Phanta EVO HS can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta EVO HS can also effectively amplify fragments up to 20 kb. Its amplification mismatch rate is 1/100 of that of ordinary Taq polymerase and 1/12 of Pfu polymerase; and the amplification speed can reach 15 seconds/kb. In addition, Phanta EVO HS has good resistance to PCR inhibitors and can be used for bacteria, fungi,
Direct PCR of plant tissue, animal tissue or whole blood samples. Strict fidelity performance and excellent amplification efficiency make Phanta EVO HS the first choice for high-fidelity PCR. Phanta EVO HS has added two monoclonal antibodies that can inhibit its 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling highly specific Hot Start PCR . The enzyme has 5'→3' polymerase activity and 3'→5' exonuclease activity, and the amplified product is blunt-ended, which is suitable for ClonExpress rapid cloning kit (C112/C113).
5 × EVO Buffer has good adaptability to PCR amplification of simple or complex templates, short fragments or long fragments. It already contains 10 mM Mg2+, you can use the MgCl2 or PCR Enhancer provided with the enzyme to optimize the reaction system. 

 

Super fidelity: the fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. Every 300,000 bases amplified, with less than 5 mismatches;
Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments;
Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of cDNA can reach 10 kb, and the effective amplification length of genome can reach 20 kb;
Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.

 

Store at -20°C

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