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Phanta EVO Super-Fidelity DNA Polymerase

NGS Library Prep kits

Molecular Research

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Number of views:
3608

Phanta EVO Super-Fidelity DNA Polymerase

Phanta Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. With the genetic engineering of Pfu DNA Polymerase, the processivity of Phanta Super-Fidelity DNA Polymerase has been greatly improved, even for very complex templates, the amplification reaction can be completed accurately and quickly. Phanta EVO Super-Fidelity DNA Polymerase It is an upgraded version of Phanta Super-Fidelity DNA Polymerase. Compared with the previous generation product, Phanta EVO has added a unique extension factor and deeply optimized the reaction system to make its amplification stability, fidelity and long-term The amplification ability of fragments has been further improved. Using simple templates such as lambda DNA and plasmids, Phanta EVO can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta EVO can also effectively amplify fragments up to 20 kb. Fragment. Its amplification mismatch rate is 1/100 of that of ordinary Taq polymerase and 1/12 of Pfu polymerase; and the amplification speed can reach 15 seconds/kb. In addition, Phanta®EVO is good for PCR inhibitors Resistance ability, can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Rigorous fidelity performance and excellent amplification efficiency make Phanta EVO a high-fidelity PCR Enzymes are the first choice. The enzyme has 5'→3' polymerase activity and 3'→5' exonuclease activity, and the amplified product is blunt-ended, suitable for ClonExpress rapid cloning kit (C112/C113). 5 × EVO Buffer has good adaptability for simple or complex template, short fragment or long fragment PCR amplification. It already contains 10 mM Mg2+, you can use the MgCl2 or PCR Enhancer provided with the enzyme to optimize the reaction system.    Effectively amplify 20 kb genome fragments and 40 kb plasmid fragments; The fidelity is 100 times that of Taq DNA Polymerase and 12 times that of Pfu DNA Polymerase; The amplification speed is 4-150 times that of conventional polymerase; The optimized buffer system is widely applicable to various types of templates, which is convenient for obtaining ideal amplification results quickly   Store at -20°C
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Number of views:
3608
Product serial number
P503-d1/d3
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Phanta Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. With the genetic engineering of Pfu DNA Polymerase, the processivity of Phanta Super-Fidelity DNA Polymerase has been greatly improved, even for very complex templates, the amplification reaction can be completed accurately and quickly. Phanta EVO Super-Fidelity DNA Polymerase It is an upgraded version of Phanta Super-Fidelity DNA Polymerase. Compared with the previous generation product, Phanta EVO has added a unique extension factor and deeply optimized the reaction system to make its amplification stability, fidelity and long-term The amplification ability of fragments has been further improved. Using simple templates such as lambda DNA and plasmids, Phanta EVO can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta EVO can also effectively amplify fragments up to 20 kb. Fragment. Its amplification mismatch rate is 1/100 of that of ordinary Taq polymerase and 1/12 of Pfu polymerase; and the amplification speed can reach 15 seconds/kb. In addition, Phanta®EVO is good for PCR inhibitors Resistance ability, can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Rigorous fidelity performance and excellent amplification efficiency make Phanta EVO a high-fidelity PCR
Enzymes are the first choice. The enzyme has 5'→3' polymerase activity and 3'→5' exonuclease activity, and the amplified product is blunt-ended, suitable for ClonExpress rapid cloning kit (C112/C113).
5 × EVO Buffer has good adaptability for simple or complex template, short fragment or long fragment PCR amplification. It already contains 10 mM Mg2+, you can use the MgCl2 or PCR Enhancer provided with the enzyme to optimize the reaction system. 

 

Effectively amplify 20 kb genome fragments and 40 kb plasmid fragments;
The fidelity is 100 times that of Taq DNA Polymerase and 12 times that of Pfu DNA Polymerase;
The amplification speed is 4-150 times that of conventional polymerase;
The optimized buffer system is widely applicable to various types of templates, which is convenient for obtaining ideal amplification results quickly

 

Store at -20°C

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