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Phanta HS Super-Fidelity DNA Polymerase

NGS Library Prep kits

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Number of views:
3519

Phanta HS Super-Fidelity DNA Polymerase

Phanta® Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. After genetic engineering of Pfu DNA Polymerase, the processivity of Phanta® Super-Fidelity DNA Polymerase has been greatly improved, even for very complex templates, it can complete the reaction accurately and quickly. The mismatch rate is 1/52 that of ordinary Taq enzyme and 1/6 that of Pfu enzyme; and the amplification speed can reach 15 seconds/kb. High fidelity and excellent amplification efficiency make Phanta® Super-Fidelity DNA Polymerase the enzyme of choice for high-fidelity PCR. On the original basis, Phanta® HS Super-Fidelity DNA Polymerase is added with two monoclonal antibodies that can inhibit 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which can perform highly specific Thermal start (Hot Start) PCR. Phanta® HS Super-Fidelity DNA Polymerase has 5’→3’ polymerase activity and 3’→5’ exonuclease activity. The amplified product is blunt-ended, suitable for ClonExpress® rapid cloning kit (C112/C113). 5 × Phanta SF reaction buffer has good adaptability for simple or complex template, short fragment or long fragment PCR amplification. The buffer already contains 10 mM Mg2+ (1 × final concentration is 2 mM). The reaction system can be optimized using the DMSO or MgSO4 provided with the enzyme. The attached PCR Enhancer can help the amplification of high GC content fragments.   Added monoclonal antibody, can perform highly specific hot-start PCR; The fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase; The amplification speed is 4-150 times that of conventional polymerase; The optimized buffer system is widely applicable to various templates; Provide PCR Enhancer to help amplify templates with high GC content   Store at -20°C
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Number of views:
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Product serial number
P502-d1/d3
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Phanta® Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. After genetic engineering of Pfu DNA Polymerase, the processivity of Phanta® Super-Fidelity DNA Polymerase has been greatly improved, even for very complex templates, it can complete the reaction accurately and quickly. The mismatch rate is 1/52 that of ordinary Taq enzyme and 1/6 that of Pfu enzyme; and the amplification speed can reach 15 seconds/kb. High fidelity and excellent amplification efficiency make Phanta® Super-Fidelity DNA Polymerase the enzyme of choice for high-fidelity PCR. On the original basis, Phanta® HS Super-Fidelity DNA Polymerase is added with two monoclonal antibodies that can inhibit 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which can perform highly specific Thermal start (Hot Start) PCR. Phanta® HS Super-Fidelity DNA Polymerase has 5’→3’ polymerase activity and 3’→5’ exonuclease activity. The amplified product is blunt-ended, suitable for ClonExpress® rapid cloning kit (C112/C113). 5 × Phanta SF reaction buffer has good adaptability for simple or complex template, short fragment or long fragment PCR amplification. The buffer already contains 10 mM Mg2+ (1 × final concentration is 2 mM). The reaction system can be optimized using the DMSO or MgSO4 provided with the enzyme. The attached PCR Enhancer can help the amplification of high GC content fragments.

 

Added monoclonal antibody, can perform highly specific hot-start PCR;
The fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase;
The amplification speed is 4-150 times that of conventional polymerase;
The optimized buffer system is widely applicable to various templates;
Provide PCR Enhancer to help amplify templates with high GC content

 

Store at -20°C

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