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Phanta Super-Fidelity DNA Polymerase

NGS Library Prep kits

Molecular Research

Drug Discovery

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Number of views:
7425

Phanta Super-Fidelity DNA Polymerase

Phanta®Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. After genetic engineering modification of Pfu DNA Polymerase, the processivity of Phanta®Super-Fidelity DNA Polymerase has been greatly improved. Even for very complex templates, the reaction can be completed accurately and quickly. Its mismatch rate is 1/52 of that of ordinary Taq enzyme and 1/6 of that of Pfu enzyme, and its amplification speed can reach 15 seconds/kb. 5 × Phanta SF reaction buffer has good adaptability for simple or complex template, short fragment or long fragment PCR amplification. The buffer already contains 10 mM Mg2+ (1 × final concentration is 2 mM). The reaction system can be optimized using the DMSO or MgSO4 provided with the enzyme. The attached PCR Enhancer can help the amplification of high GC content fragments.   The fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase; The amplification speed is 4-150 times that of conventional polymerase; Wide template adaptability; The optimized buffer system is widely applicable to various types of templates, which is convenient for obtaining ideal amplification results quickly   Store at -20°C
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Number of views:
7425
Product serial number
P501-d1/d2/d3
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Phanta®Super-Fidelity DNA Polymerase is a new generation of ultra-fidelity DNA polymerase modified from Pfu DNA Polymerase. It has extremely high amplification efficiency and wide template adaptability, and can be used in almost all PCR reactions. After genetic engineering modification of Pfu DNA Polymerase, the processivity of Phanta®Super-Fidelity DNA Polymerase has been greatly improved. Even for very complex templates, the reaction can be completed accurately and quickly. Its mismatch rate is 1/52 of that of ordinary Taq enzyme and 1/6 of that of Pfu enzyme, and its amplification speed can reach 15 seconds/kb. 5 × Phanta SF reaction buffer has good adaptability for simple or complex template, short fragment or long fragment PCR amplification. The buffer already contains 10 mM Mg2+ (1 × final concentration is 2 mM). The reaction system can be optimized using the DMSO or MgSO4 provided with the enzyme. The attached PCR Enhancer can help the amplification of high GC content fragments.

 

The fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase;
The amplification speed is 4-150 times that of conventional polymerase;
Wide template adaptability;
The optimized buffer system is widely applicable to various types of templates, which is convenient for obtaining ideal amplification results quickly

 

Store at -20°C

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