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VAHTS Universal Plus DNA Library Prep Kit for Illumina® is specially designed for DNA library preparation for next generation sequencing (NGS) on Illumina® platforms. This kit combines DNA fragmentation, end repair and dA tailing into one step. The products can be directly used to adapter Ligation without purification, then for library enrichment and size selection. This kit is suitable for library preparation from 100 pg - 1 μg of input DNA. No mechanical fragmentation of the genome simplifies the process of library construction and shortens the operation time, suitable for the construction of PCR and PCR-Free libraries. This kit is perfectly compatible with DNA from different sources and different inputs, and the required fragment size library can be obtained by adjusting the fragmentation time according to the size of the target insert. All kit components are subjected to stringent functional quality control, ensuring the consistency and reproducibility of library preparation.
1. Excellent template type compatibility;
2. Broad input volume compatibility;
3. Excellent library conversion;
4. High quality sequencing data.
|Components||ND617- 01 (24 rxn)||ND617- 02 (96 rxn)|
FEA Enzyme Mix
Rapid Ligation Buffer 3
Rapid DNA Ligase
VAHTS HiFi Amplification Mix
PCR Primer Mix 3 for Illumina
Control DNA (100 ng/μl)
4 × 600 μl
4 × 600 μl
▲Control DNA is Salmon genomic DNA.
All components should be stored at - 30℃ ~ - 15℃, and transported at - 20℃ ~ 0℃.