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VAHTS Universal DNA Library Prep Kit for Illumina V4 is a library preparation kit targeted for Illumina high-throughput sequencing platform optimization. 100 pg-1 μg of fragmented double-stranded DNA can be converted into a dedicated library for Illumina high-throughput sequencing platform. As a new generation of upgraded version, the kit can improve the conversion rate of low-quality template libraries and reduce the repetition rate of libraries by optimizing and improving the terminal repair module, connecting module and library amplification module. This kit is widely suitable for PCR or PCR-free library preparation of a variety of samples, and is compatible with the targeted capture process. All reagents provided in the kit have undergone strict quality control and functional validation to ensure the stability and repeatability of library preparation to the greatest extent.