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VAHTS® RNA Multi-PCR Library Prep Kit is based on multiplex PCR and can be used for RNA sequencing library preparation. This kit contains reverse transcription module and multi-PCR based library preparation module. Two rounds of PCR amplification are adopted to complete target amplification and enrichment. It only takes 5 h from viral genomic sample acquisition to obtaining complete sequencing library, and the manual operation time is less than 1.5 h. This product is compatible with the starting template range of 16 CT - 38 CT, and the constructed library is suitable for sequencing analysis on the Illumina platform. The customized panel in this kit has a coverage of 99.7% of the whole genome. With multi-PCR amplification reagents, the uniformity can reach 90%, which can effectively detect the mutation and deletion sites. The optimized combination of the reagents enables easy, stable and reliable RNA library construction. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the optimal stability and repeatability of library construction.