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Taq Pro Universal SYBR qPCR Master Mix Q712

Taq Pro Universal SYBR qPCR Master Mix Q712

SKU: #001 - In Stock
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Product Description

This product is a special premix for qPCR reaction using SYBR Green I chimeric fluorescence method. The core component, Taq Pro DNA Polymerase, is a new generation of hot-start polymerase modified by antibody method. It has many advantages such as strong specificity, high detection sensitivity and high amplification yield. With the combination of Buffer optimized for qPCR and specific enhancer, it is very suitable for qPCR reaction with high specificity and sensitivity. This kit contains Specific ROX Reference Dye, which is suitable for all qPCR instruments without adjusting the concentration of ROX on different instruments. This kit contains 2 × master mix that can be amplified by adding primers and template.

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Product Features

1. Ultra high plateau value
2. Perfect balance between sensitivity and specificity
3. All platform universality

Key Data

Ultra high plateau value

Hela cDNA was used as template to amplify six different genes by Q712 and SYBR qPCR reagents of other brands (from supplier A, B, C, D).

Perfect balance between sensitivity and specificity

Fig. A shows eight 10-fold gradient dilutions of pUC19 plasmid and Fig. B shows the standard curve obtained according to the CT values in Fig. A. In Fig C, cDNA of HeLa cells was used as a template, six different genes were amplified under the same reaction conditions using Q712 and SYBR qPCR reagents of other brands (from supplier A, B, C, D).

All platform universality

Q712 was used to amplify EGFR gene on different qPCR instruments (Type: ABI Stepone, ABI QuantStudio 3, and Roche LightCycler 480) with excellent quantitative results.

Components

Components Q712-02 (500 rxns/20 μl reaction) Q712-03 (2,500 rxns/20 μl reaction)
2 × Taq Pro Universal SYBR qPCR Master Mix* 4 × 1.25 ml 5 × Q712-02

* It contains dNTP, Mg2+, Taq Pro DNA Polymerase, SYBR Green I and Specific ROX Reference Dye, etc.

Storage

Store at -30 ~ -15℃  and protect from light  for up to 18 months. Master mix can be stored stably at 2 ~ 8℃ for 6 months and protected from light after thawing. Transport at ≤ 0℃.

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