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The Taq Pro Universal SYBR qPCR Master Mix is a special master mix for qPCR by SYBR Green I chimeric fluorescence method. The Taq Pro DNA Polymerase used in this product is a new generation hot start polymerase that is upgraded and modified using an antibody method, with the advantages of high specificity, high detection sensitivity and high amplification yield. Combined with the optimal buffer optimized for qPCR and the patented specific promoting factors, it greatly improves the qPCR amplification yield, with a ultra-high plateau value, and is suitable for qPCR amplification with high specificity and sensitivity. Taq Pro Universal SYBR qPCR Master Mix contains a unique ROX Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments.
1. Ultra high plateau value
2. Perfect balance between sensitivity and specificity
3. All platform universality
Ultra high plateau value
Hela cDNA was used as template to amplify six different genes by Q712 and SYBR qPCR reagents of other brands (from supplier A, B, C, D).
Perfect balance between sensitivity and specificity
Fig. A shows eight 10-fold gradient dilutions of pUC19 plasmid and Fig. B shows the standard curve obtained according to the CT values in Fig. A. In Fig C, cDNA of HeLa cells was used as a template, six different genes were amplified under the same reaction conditions using Q712 and SYBR qPCR reagents of other brands (from supplier A, B, C, D).
All platform universality
Q712 was used to amplify EGFR gene on different qPCR instruments (Type: ABI Stepone, ABI QuantStudio 3, and Roche LightCycler 480) with excellent quantitative results.
|Components||Q712-02 (500 rxns/20 μl reaction)||Q712-03 (2,500 rxns/20 μl reaction)|
|2 × Taq Pro Universal SYBR qPCR Master Mix*||4 × 1.25 ml||5 × Q712-02|
* It contains dNTP, Mg2+, Taq Pro DNA Polymerase, SYBR Green I and Specific ROX Reference Dye, etc.
Store at -30 ~ -15℃ and protect from light. Master mix can be stored stably at 2 ~ 8℃ for 6 months and protected from light after thawing. Transport at ≤ 0℃.
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