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T7 High Yield RNA Transcription Kit TR101
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Product Introduction
T7 High Yield Transcription Kit has optimized the transcription reaction system. T7 RNA Polymerase synthesizes RNA complementary to one strand of DNA from the downstream of the template DNA T7 promoter. It is simple and fast to obtain a large number of RNA molecules, which can be in the substrate during transcription. Add modified nucleotides to prepare biotin or dye-labeled RNA.
This kit can produce 150-200 μg of RNA with 0.5 μg of template input. The RNA synthesized by transcription can be used for research such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation. Aspects of downstream applications.
Product Advantages
Optimized reaction system;
Each reaction produces up to 150-200 μg of RNA product
Components
Storage
Store at -30 ~ -15℃，transport at ≤0℃.
Citation
Lyu, Jialan, et al. "The conserved microRNA miR-210 regulates lipid metabolism and photoreceptor maintenance in the Drosophila retina." Cell Death & Differentiation 28.2 (2021): 764-779.Impact Factor:15.828
Sui, Baokun, et al. "A novel antiviral lncRNA, EDAL, shields a T309 O-GlcNAcylation site to promote EZH2 lysosomal degradation." Genome biology 21.1 (2020): 1-36.Impact Factor:13.583
Chen, Peng, et al. "A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing." Genome biology 21.1 (2020): 1-13.Impact Factor:13.583
Feng, Yue, et al. "METTL3/METTL14 Transactivation and m6A-Dependent TGF-β1 Translation in Activated Kupffer Cells." Cellular and Molecular Gastroenterology and Hepatology 12.3 (2021): 839-856.Impact Factor:9.225
Zhao, Jing, et al. "Proteomic analyses of whitefly-begomovirus interactions reveal the inhibitory role of tumorous imaginal discs in viral retention." Frontiers in immunology (2020): 1596.Impact Factor:7.561
Application Notes Abstract
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