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T7 High Yield Transcription Kit has optimized the transcription reaction system. T7 RNA Polymerase synthesizes RNA complementary to one strand of DNA from the downstream of the template DNA T7 promoter. It is simple and fast to obtain a large number of RNA molecules, which can be in the substrate during transcription. Add modified nucleotides to prepare biotin or dye-labeled RNA.
This kit can produce 150-200 μg of RNA with 0.5 μg of template input. The RNA synthesized by transcription can be used for research such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation. Aspects of downstream applications.
Optimized reaction system;
Each reaction produces up to 150-200 μg of RNA product
Store at -30 ~ -15℃，transport at ≤0℃.
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