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In the presence of templates and primers, T4 DNA polymerase catalyzes the synthesis of DNA along the 5´→3´ direction. This enzyme also has 3´→5´ exonuclease activity, which is stronger than DNA polymerase I. Unlike DNA polymerase I, T4 DNA polymerase does not have 5´→3´ exonuclease activity.
Connect the sticky end or blunt end quickly in 5 minutes at room temperature;
dsDNA cut repair
Store at -30 ~ -15℃ for up to 24 months, transportation at ≤ 0℃.