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T4 DNA Ligase catalyzes the formation of phosphodiester bonds between adjacent 5´-phosphate ends and 3´-hydroxyl ends on double-stranded DNA or RNA. The enzyme not only catalyzes the connection between blunt ends or sticky ends, but also repairs single-strand cuts in double-stranded DNA, RNA, or DNA/RNA hybrids.
3' end with dA tail;
Prepare probes with random primers;
Random primer labeling