Click and submit your question and we will get back to you within one business day
Single Cell Full Length mRNA Amplification Kit can achieve enough amount of full-length cDNA through using 1 – 500 of cells or 10 pg – 10 ng of total RNA as templates. In this kit, Oligo (dT) VN Primer is used as reverse transcription primer and template-switching activity of Sc Reverse Transcriptase is utilized to add adapter sequences at the 3′ of cDNA. Subsequently, PCR amplification is performed through the adapter sequences to obtain full-length cDNA amplification products, which effectively avoids 3′ preference and rRNA contamination in the cDNA synthesis process. The obtained full-length cDNA amplification products can be used for genetic regulation information analysis such as differential gene expression, alternative splicing, and fusion genes. Single Cell Full Length mRNA Amplification Kit is compatible with sample volumes within 2.5 μl. Generally, 2 – 20 ng of cDNA amplification products can be obtained in one reaction.